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Title: Structure, mineralogy, and microbial diversity of geothermal spring microbialites associated with a deep oil drilling in Romania

Abstract

Modern mineral deposits play an important role in evolutionary studies by providing clues to the formation of ancient lithified microbial communities. Here we report the presence of microbialite-forming microbial mats in different microenvironments at 32°C, 49°C, and 65°C around the geothermal spring from an abandoned oil drill in Ciocaia, Romania. The mineralogy and the macro- and microstructure of the microbialites were investigated, together with their microbial diversity based on a 16S rRNA gene amplicon sequencing approach. The calcium carbonate is deposited mainly in the form of calcite. At 32°C and 49°C, the microbialites show a laminated structure with visible microbial mat-carbonate crystal interactions. At 65°C, the mineral deposit is clotted, without obvious organic residues. Partial 16S rRNA gene amplicon sequencing showed that the relative abundance of the phylum Archaea was low at 32°C (<0.5%) but increased significantly at 65°C (36%). The bacterial diversity was either similar to other microbialites described in literature (the 32°C sample) or displayed a specific combination of phyla and classes (the 49°C and 65°C samples). Bacterial taxa were distributed among 39 phyla, out of which 14 had inferred abundances >1%. The dominant bacterial groups at 32°C were Cyanobacteria, Gammaproteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, Thermi, Actinobacteria, Planctomycetes, andmore » Defferibacteres. At 49°C, there was a striking dominance of the Gammaproteobacteria, followed by Firmicutes, Bacteroidetes, and Armantimonadetes. The 65°C sample was dominated by Betaproteobacteria, Firmicutes, [OP1], Defferibacteres, Thermi, Thermotogae, [EM3], and Nitrospirae. Lastly, several groups from Proteobacteria and Firmicutes, together with Halobacteria and Melainabacteria were described for the first time in calcium carbonate deposits. Overall, the spring from Ciocaia emerges as a valuable site to probe microbes-minerals interrelationships along thermal and geochemical gradients.« less

Authors:
 [1];  [1];  [2];  [3];  [1];  [4];  [4];  [4];  [5];  [6]
  1. National Inst. of Research and Development for Biological Sciences, Cluj-Napoca (Romania). Inst. of Biological Research; Babe,s-Bolyai Univ., Cluj-Napoca (Romania)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Colorado State Univ., Fort Collins, CO (United States). Fish, Wildlife, and Conservation Biology
  3. Babe,s-Bolyai Univ., Cluj-Napoca (Romania); Kazan Federal Univ., Tatarstan (Russia)
  4. Babe,s-Bolyai Univ., Cluj-Napoca (Romania)
  5. Biological Research Center, Jibou (Romania)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1260667
Grant/Contract Number:  
PD104/2012; PN09-360201
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; hotsprings; carbonate; biomineralization; amplicon sequencing; microbial diversity; oil drill

Citation Formats

Coman, Cristian, Chiriac, Cecilia M., Robeson, Michael S., Ionescu, Corina, Dragos, Nicolae, Barbu-Tudoran, Lucian, Andrei, Adrian-Åžtefan, Banciu, Horia L., Sicora, Cosmin, and Podar, Mircea. Structure, mineralogy, and microbial diversity of geothermal spring microbialites associated with a deep oil drilling in Romania. United States: N. p., 2015. Web. doi:10.3389/fmicb.2015.00253.
Coman, Cristian, Chiriac, Cecilia M., Robeson, Michael S., Ionescu, Corina, Dragos, Nicolae, Barbu-Tudoran, Lucian, Andrei, Adrian-Åžtefan, Banciu, Horia L., Sicora, Cosmin, & Podar, Mircea. Structure, mineralogy, and microbial diversity of geothermal spring microbialites associated with a deep oil drilling in Romania. United States. doi:10.3389/fmicb.2015.00253.
Coman, Cristian, Chiriac, Cecilia M., Robeson, Michael S., Ionescu, Corina, Dragos, Nicolae, Barbu-Tudoran, Lucian, Andrei, Adrian-Åžtefan, Banciu, Horia L., Sicora, Cosmin, and Podar, Mircea. Mon . "Structure, mineralogy, and microbial diversity of geothermal spring microbialites associated with a deep oil drilling in Romania". United States. doi:10.3389/fmicb.2015.00253. https://www.osti.gov/servlets/purl/1260667.
@article{osti_1260667,
title = {Structure, mineralogy, and microbial diversity of geothermal spring microbialites associated with a deep oil drilling in Romania},
author = {Coman, Cristian and Chiriac, Cecilia M. and Robeson, Michael S. and Ionescu, Corina and Dragos, Nicolae and Barbu-Tudoran, Lucian and Andrei, Adrian-Åžtefan and Banciu, Horia L. and Sicora, Cosmin and Podar, Mircea},
abstractNote = {Modern mineral deposits play an important role in evolutionary studies by providing clues to the formation of ancient lithified microbial communities. Here we report the presence of microbialite-forming microbial mats in different microenvironments at 32°C, 49°C, and 65°C around the geothermal spring from an abandoned oil drill in Ciocaia, Romania. The mineralogy and the macro- and microstructure of the microbialites were investigated, together with their microbial diversity based on a 16S rRNA gene amplicon sequencing approach. The calcium carbonate is deposited mainly in the form of calcite. At 32°C and 49°C, the microbialites show a laminated structure with visible microbial mat-carbonate crystal interactions. At 65°C, the mineral deposit is clotted, without obvious organic residues. Partial 16S rRNA gene amplicon sequencing showed that the relative abundance of the phylum Archaea was low at 32°C (<0.5%) but increased significantly at 65°C (36%). The bacterial diversity was either similar to other microbialites described in literature (the 32°C sample) or displayed a specific combination of phyla and classes (the 49°C and 65°C samples). Bacterial taxa were distributed among 39 phyla, out of which 14 had inferred abundances >1%. The dominant bacterial groups at 32°C were Cyanobacteria, Gammaproteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, Thermi, Actinobacteria, Planctomycetes, and Defferibacteres. At 49°C, there was a striking dominance of the Gammaproteobacteria, followed by Firmicutes, Bacteroidetes, and Armantimonadetes. The 65°C sample was dominated by Betaproteobacteria, Firmicutes, [OP1], Defferibacteres, Thermi, Thermotogae, [EM3], and Nitrospirae. Lastly, several groups from Proteobacteria and Firmicutes, together with Halobacteria and Melainabacteria were described for the first time in calcium carbonate deposits. Overall, the spring from Ciocaia emerges as a valuable site to probe microbes-minerals interrelationships along thermal and geochemical gradients.},
doi = {10.3389/fmicb.2015.00253},
journal = {Frontiers in Microbiology},
number = ,
volume = 6,
place = {United States},
year = {2015},
month = {3}
}

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