Tight regulation of plant immune responses by combining promoter and suicide exon elements

Gonzalez, Tania L.; Liang, Yan; Nguyen, Bao N.; Staskawicz, Brian J.; Loqué, Dominique; Hammond, Ming C.

doi:10.1093/nar/gkv655

Title: Tight regulation of plant immune responses by combining promoter and suicide exon elements

Journal Article · Thu Jul 02 04:00:00 UTC 2015 · Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gkv655 · OSTI ID:1257376

Gonzalez, Tania L. ^[1]; Liang, Yan ^[2]; Nguyen, Bao N. ^[1]; Staskawicz, Brian J. ^[1]; Loqué, Dominique ^[2]; Hammond, Ming C. ^[3]

Univ. of California, Berkeley, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Effector-triggered immunity (ETI) is activated when plant disease resistance (R) proteins recognize the presence of pathogen effector proteins delivered into host cells. The ETI response generally encompasses a defensive ‘hypersensitive response’ (HR) that involves programmed cell death at the site of pathogen recognition. While many R protein and effector protein pairs are known to trigger HR, other components of the ETI signaling pathway remain elusive. Effector genes regulated by inducible promoters cause background HR due to leaky protein expression, preventing the generation of relevant transgenic plant lines. By employing the HyP5SM suicide exon, we have developed a strategy to tightly regulate effector proteins such that HR is chemically inducible and non-leaky. This alternative splicing-based gene regulation system was shown to successfully control Bs2/AvrBs2-dependent and RPP1/ATR1Δ51-dependent HR in Nicotiana benthamiana and Nicotiana tabacum, respectively. It was also used to generate viable and healthy transgenic Arabidopsis thaliana plants that inducibly initiate HR. In conclusion, beyond enabling studies on the ETI pathway, our regulatory strategy is generally applicable to reduce or eliminate undesired background expression of transgenes.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1257376

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 14 Vol. 43; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Fungal Effector Proteins Stergiopoulos, Ioannis; de Wit, Pierre J. G. M. Annual Review of Phytopathology, Vol. 47, Issue 1 https://doi.org/10.1146/annurev.phyto.112408.132637	journal	September 2009
Network Properties of Robust Immunity in Plants Tsuda, Kenichi; Sato, Masanao; Stoddard, Thomas PLoS Genetics, Vol. 5, Issue 12 https://doi.org/10.1371/journal.pgen.1000772	journal	December 2009
Transgenic Resistance Confers Effective Field Level Control of Bacterial Spot Disease in Tomato Horvath, Diana M.; Stall, Robert E.; Jones, Jeffrey B. PLoS ONE, Vol. 7, Issue 8 https://doi.org/10.1371/journal.pone.0042036	journal	August 2012
The Arabidopsis miR472-RDR6 Silencing Pathway Modulates PAMP- and Effector-Triggered Immunity through the Post-transcriptional Control of Disease Resistance Genes Boccara, Martine; Sarazin, Alexis; Thiébeauld, Odon PLoS Pathogens, Vol. 10, Issue 1 https://doi.org/10.1371/journal.ppat.1003883	journal	January 2014
NB-LRR proteins: pairs, pieces, perception, partners, and pathways L., Dangl, Jeffery; K., Eitas, Timothy The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/12e8-wx51	text	January 2010
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Characterization of eds1, a Mutation in Arabidopsis Suppressing Resistance to Peronospora parasitica Specified by Several Different RPP Genes Parker, Jane E.; Holub, Eric B.; Frost, Louise N. The Plant Cell, Vol. 8, Issue 11 https://doi.org/10.2307/3870410	journal	November 1996
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The Arabidopsis miR472-RDR6 Silencing Pathway Modulates PAMP- and Effector-Triggered Immunity through the Post-transcriptional Control of Disease Resistance Genes Boccara, Martine; Sarazin, Alexis; Thiébeauld, Odon ETH Zurich https://doi.org/10.3929/ethz-b-000082845	text	January 2014

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