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Title: Abiotic protein fragmentation by manganese oxide: Implications for a mechanism to supply soil biota with oligopeptides

Proteins facilitate a wide range of chemical transformations important in soil as well as being a major reservoir of soil nitrogen themselves. The interactions and reactions of proteins with soils and minerals are of key importance to our understanding of their functional persistence in the environment. We combined NMR and EPR spectroscopies to distinguish the reaction of a model protein with a redox active mineral surface (Birnessite, MnO 2) from its response to a redox neutral phyllosilicate (Kaolinite). Our data demonstrate that birnessite fragments the model protein while kaolinite has little impact on the protein structure. NMR and EPR spectroscopies are shown to be valuable tools to observe these reactions and capture the extent of protein transformation together with the extent of mineral response. These data suggest that mineral surfaces can have both promoting and retarding roles in terrestrial nitrogen cycling, with redox active minerals acting as accelerators by catalyzing the breakdown of proteins and proteinaceous materials while phyllosilicates are more likely to act as preservative media.
 [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [3]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Oregon State Univ., Corvallis, OR (United States)
  3. Oregon State Univ., Corvallis, OR (United States); Institut fur Bodenlandschaftforschung, Leibniz Zentrum fur Agrarlandschaftsforschung (ZALF), Muncheberg (Germany)
Publication Date:
Report Number(s):
Journal ID: ISSN 0013-936X; 48183; 48364; KP1704020
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 50; Journal Issue: 7; Journal ID: ISSN 0013-936X
American Chemical Society (ACS)
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; Environmental Molecular Sciences Laboratory
OSTI Identifier: