Abiotic protein fragmentation by manganese oxide: Implications for a mechanism to supply soil biota with oligopeptides
Abstract
The ability of plants and microorganisms to take up organic nitrogen in the form of free amino acids and oligopeptides has received increasing attention over the last two decades, yet the mechanisms for the formation of such compounds in soil environments remain poorly understood. We used Nuclear Magnetic Resonance (NMR) and Electron Paramagnetic Resonance (EPR) spectroscopies to distinguish the reaction of a model protein with a pedogenic oxide (Birnessite, MnO2) from its response to a phyllosilicate (Kaolinite). Our data demonstrate that birnessite fragments the model protein while kaolinite does not, resulting in soluble peptides that would be available to soil biota and confirming the existence of an abiotic pathway for the formation of organic nitrogen compounds for direct uptake by plants and microorganisms. The absence of reduced Mn(II) in the solution suggests that birnessite acts as a catalyst rather than an oxidant in this reaction. NMR and EPR spectroscopies are shown to be valuable tools to observe these reactions and capture the extent of protein transformation together with the extent of mineral response.
- Authors:
-
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Oregon State Univ., Corvallis, OR (United States)
- Oregon State Univ., Corvallis, OR (United States); Institut fur Bodenlandschaftforschung, Leibniz Zentrum fur Agrarlandschaftsforschung (ZALF), Muncheberg (Germany)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1254585
- Alternate Identifier(s):
- OSTI ID: 1580807
- Report Number(s):
- PNNL-SA-108407
Journal ID: ISSN 0013-936X; 48183; 48364; KP1704020
- Grant/Contract Number:
- AC05-76RL01830; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 50; Journal Issue: 7; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Environmental Molecular Sciences Laboratory
Citation Formats
Reardon, Patrick N., Chacon, Stephany S., Walter, Eric D., Bowden, Mark E., Washton, Nancy M., and Kleber, Markus W. Abiotic protein fragmentation by manganese oxide: Implications for a mechanism to supply soil biota with oligopeptides. United States: N. p., 2016.
Web. doi:10.1021/acs.est.5b04622.
Reardon, Patrick N., Chacon, Stephany S., Walter, Eric D., Bowden, Mark E., Washton, Nancy M., & Kleber, Markus W. Abiotic protein fragmentation by manganese oxide: Implications for a mechanism to supply soil biota with oligopeptides. United States. https://doi.org/10.1021/acs.est.5b04622
Reardon, Patrick N., Chacon, Stephany S., Walter, Eric D., Bowden, Mark E., Washton, Nancy M., and Kleber, Markus W. Mon .
"Abiotic protein fragmentation by manganese oxide: Implications for a mechanism to supply soil biota with oligopeptides". United States. https://doi.org/10.1021/acs.est.5b04622. https://www.osti.gov/servlets/purl/1254585.
@article{osti_1254585,
title = {Abiotic protein fragmentation by manganese oxide: Implications for a mechanism to supply soil biota with oligopeptides},
author = {Reardon, Patrick N. and Chacon, Stephany S. and Walter, Eric D. and Bowden, Mark E. and Washton, Nancy M. and Kleber, Markus W.},
abstractNote = {The ability of plants and microorganisms to take up organic nitrogen in the form of free amino acids and oligopeptides has received increasing attention over the last two decades, yet the mechanisms for the formation of such compounds in soil environments remain poorly understood. We used Nuclear Magnetic Resonance (NMR) and Electron Paramagnetic Resonance (EPR) spectroscopies to distinguish the reaction of a model protein with a pedogenic oxide (Birnessite, MnO2) from its response to a phyllosilicate (Kaolinite). Our data demonstrate that birnessite fragments the model protein while kaolinite does not, resulting in soluble peptides that would be available to soil biota and confirming the existence of an abiotic pathway for the formation of organic nitrogen compounds for direct uptake by plants and microorganisms. The absence of reduced Mn(II) in the solution suggests that birnessite acts as a catalyst rather than an oxidant in this reaction. NMR and EPR spectroscopies are shown to be valuable tools to observe these reactions and capture the extent of protein transformation together with the extent of mineral response.},
doi = {10.1021/acs.est.5b04622},
journal = {Environmental Science and Technology},
number = 7,
volume = 50,
place = {United States},
year = {Mon Mar 14 00:00:00 EDT 2016},
month = {Mon Mar 14 00:00:00 EDT 2016}
}
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Works referencing / citing this record:
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