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Title: High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density

Journal Article · · Journal of Structural Biology

Not Available

Sponsoring Organization:
USDOE Office of Science (SC), Office of Workforce Development for Teachers & Scientists (WDTS); USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-98CH10886; SC0012704
OSTI ID:
1250356
Journal Information:
Journal of Structural Biology, Journal Name: Journal of Structural Biology Journal Issue: 1 Vol. 191; ISSN 1047-8477
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

References (42)

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Fragment library design: efficiently hunting drugs in chemical space journal October 2010
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Binding of hydroxamic acid inhibitors to crystalline thermolysin suggests a pentacoordinate zinc intermediate in catalysis journal November 1981
Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures journal May 2011
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Design and Characterization of Libraries of Molecular Fragments for Use in NMR Screening against Protein Targets journal November 2004
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Missing Fragments: Detecting Cooperative Binding in Fragment-Based Drug Design journal February 2012
High-throughput crystallography for lead discovery in drug design journal January 2002
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HKL2MAP : a graphical user interface for macromolecular phasing with SHELX programs journal September 2004
A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose journal September 2009
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Overview of the CCP 4 suite and current developments journal March 2011
Development of high-performance X-ray transparent crystallization plates for in situ protein crystal screening and analysis journal June 2011
In-plate protein crystallization, in situ ligand soaking and X-ray diffraction journal August 2011
Optimal fine ϕ-slicing for single-photon-counting pixel detectors journal December 2011
In situ macromolecular crystallography using microbeams journal April 2012
Nanolitre-scale crystallization using acoustic liquid-transfer technology journal July 2012
CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films journal September 2012
On the reproducibility of protein crystal structures: five atomic resolution structures of trypsin journal July 2013
Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines journal August 2013
Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes journal April 2014
Exploiting fast detectors to enter a new dimension in room-temperature crystallography journal April 2014
Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening journal January 2015
Solvent minimization induces preferential orientation and crystal clustering in serial micro-crystallography on micro-meshes, in situ plates and on a movable crystal conveyor belt journal October 2014
A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids journal November 2014
Bioactive Contaminants Leach from Disposable Laboratory Plasticware journal November 2008
Fragment-Based Screening by Protein Crystallography: Successes and Pitfalls journal October 2012

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