Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite
Abstract
Alkaline flocculation holds great potential as a low-cost harvesting method for marine microalgae biomass production. Alkaline flocculation is induced by an increase in pH and is related to precipitation of calcium and magnesium salts. In this study, we used the diatom Phaeodactylum tricornutum as model organism to study alkaline flocculation of marine microalgae cultured in seawater medium. Flocculation started when pH was increased to 10 and flocculation efficiency reached 90% when pH was 10.5, which was consistent with precipitation modeling for brucite or Mg(OH)2. Compared to freshwater species, more magnesium is needed to achieve flocculation (>7.5 mM). Zeta potential measurements suggest that brucite precipitation caused flocculation by charge neutralization. When calcium concentration was 12.5 mM, flocculation was also observed at a pH of 10. Furthermore, zeta potential remained negative up to pH 11.5, suggesting that precipitated calcite caused flocculation by a sweeping coagulation mechanism.
- Authors:
-
- KU Leuven Kulak, Kortrijk (Belgium)
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- KU Leuven, Leuven (Belgium)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
- OSTI Identifier:
- 1236222
- Alternate Identifier(s):
- OSTI ID: 1250331
- Report Number(s):
- SAND-2015-6106J
Journal ID: ISSN 0960-8524; 615250
- Grant/Contract Number:
- AC04-94AL85000; 9.1.1.3_DE-EE0005995
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioresource Technology
- Additional Journal Information:
- Journal Volume: 196; Journal Issue: C; Journal ID: ISSN 0960-8524
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; coagulation; marine algae; enmeshment; biomass; autoflocculation
Citation Formats
Vandamme, Dries, Pohl, Philip I., Beuckels, Annelies, Foubert, Imogen, Brady, Patrick Vane, Muylaert, Koenraad, and Hewson, John C. Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite. United States: N. p., 2015.
Web. doi:10.1016/j.biortech.2015.08.042.
Vandamme, Dries, Pohl, Philip I., Beuckels, Annelies, Foubert, Imogen, Brady, Patrick Vane, Muylaert, Koenraad, & Hewson, John C. Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite. United States. https://doi.org/10.1016/j.biortech.2015.08.042
Vandamme, Dries, Pohl, Philip I., Beuckels, Annelies, Foubert, Imogen, Brady, Patrick Vane, Muylaert, Koenraad, and Hewson, John C. Thu .
"Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite". United States. https://doi.org/10.1016/j.biortech.2015.08.042. https://www.osti.gov/servlets/purl/1236222.
@article{osti_1236222,
title = {Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite},
author = {Vandamme, Dries and Pohl, Philip I. and Beuckels, Annelies and Foubert, Imogen and Brady, Patrick Vane and Muylaert, Koenraad and Hewson, John C.},
abstractNote = {Alkaline flocculation holds great potential as a low-cost harvesting method for marine microalgae biomass production. Alkaline flocculation is induced by an increase in pH and is related to precipitation of calcium and magnesium salts. In this study, we used the diatom Phaeodactylum tricornutum as model organism to study alkaline flocculation of marine microalgae cultured in seawater medium. Flocculation started when pH was increased to 10 and flocculation efficiency reached 90% when pH was 10.5, which was consistent with precipitation modeling for brucite or Mg(OH)2. Compared to freshwater species, more magnesium is needed to achieve flocculation (>7.5 mM). Zeta potential measurements suggest that brucite precipitation caused flocculation by charge neutralization. When calcium concentration was 12.5 mM, flocculation was also observed at a pH of 10. Furthermore, zeta potential remained negative up to pH 11.5, suggesting that precipitated calcite caused flocculation by a sweeping coagulation mechanism.},
doi = {10.1016/j.biortech.2015.08.042},
journal = {Bioresource Technology},
number = C,
volume = 196,
place = {United States},
year = {Thu Aug 20 00:00:00 EDT 2015},
month = {Thu Aug 20 00:00:00 EDT 2015}
}
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Works referencing / citing this record:
Harvesting of Microalgae for Biomass Production
book, January 2019
- Roselet, Fabio; Vandamme, Dries; Muylaert, Koenraad
- Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment