skip to main content

DOE PAGESDOE PAGES

Title: Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica

Yarrowia lipolytica is an oleaginous ascomycete yeast that stores lipids in response to limitation of nitrogen. Furthermore, while the enzymatic pathways responsible for neutral lipid accumulation in Y. lipolytica are well characterized, regulation of these pathways has received little attention. We therefore sought to characterize the response to nitrogen limitation at system-wide levels, including the proteome, phosphoproteome and metabolome, to better understand how this organism regulates and controls lipid metabolism and to identify targets that may be manipulated to improve lipid yield.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Report Number(s):
PNNL-SA-112784
Journal ID: ISSN 1471-2164; PII: 2471
Grant/Contract Number:
AC05-76RL01830; SC0008744
Type:
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 17; Journal Issue: 1; Journal ID: ISSN 1471-2164
Publisher:
Springer
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Yarrowia lipolytica; lipid; proteome; metabolome; phosphorylation; phosphoproteome; nitrogen regulation; beta-oxidation; ribosome biogenesis; translation
OSTI Identifier:
1249359

Pomraning, Kyle R., Kim, Young -Mo, Nicora, Carrie D., Chu, Rosalie K., Bredeweg, Erin L., Purvine, Samuel O., Hu, Dehong, Metz, Thomas O., and Baker, Scott E.. Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica. United States: N. p., Web. doi:10.1186/s12864-016-2471-2.
Pomraning, Kyle R., Kim, Young -Mo, Nicora, Carrie D., Chu, Rosalie K., Bredeweg, Erin L., Purvine, Samuel O., Hu, Dehong, Metz, Thomas O., & Baker, Scott E.. Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica. United States. doi:10.1186/s12864-016-2471-2.
Pomraning, Kyle R., Kim, Young -Mo, Nicora, Carrie D., Chu, Rosalie K., Bredeweg, Erin L., Purvine, Samuel O., Hu, Dehong, Metz, Thomas O., and Baker, Scott E.. 2016. "Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica". United States. doi:10.1186/s12864-016-2471-2. https://www.osti.gov/servlets/purl/1249359.
@article{osti_1249359,
title = {Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica},
author = {Pomraning, Kyle R. and Kim, Young -Mo and Nicora, Carrie D. and Chu, Rosalie K. and Bredeweg, Erin L. and Purvine, Samuel O. and Hu, Dehong and Metz, Thomas O. and Baker, Scott E.},
abstractNote = {Yarrowia lipolytica is an oleaginous ascomycete yeast that stores lipids in response to limitation of nitrogen. Furthermore, while the enzymatic pathways responsible for neutral lipid accumulation in Y. lipolytica are well characterized, regulation of these pathways has received little attention. We therefore sought to characterize the response to nitrogen limitation at system-wide levels, including the proteome, phosphoproteome and metabolome, to better understand how this organism regulates and controls lipid metabolism and to identify targets that may be manipulated to improve lipid yield.},
doi = {10.1186/s12864-016-2471-2},
journal = {BMC Genomics},
number = 1,
volume = 17,
place = {United States},
year = {2016},
month = {2}
}

Works referenced in this record:

Lipid particle composition of the yeast Yarrowia lipolytica depends on the carbon source
journal, March 2006
  • Athenstaedt, Karin; Jolivet, Pascale; Boulard, CĂ©line
  • PROTEOMICS, Vol. 6, Issue 5, p. 1450-1459
  • DOI: 10.1002/pmic.200500339

Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry
journal, November 2006
  • Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou
  • Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801
  • DOI: 10.1021/ac061133r

Physiology and genetics of the dimorphic fungus Yarrowia lipolytica
journal, April 1997

Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production
journal, January 2013

Transcriptomic Analyses during the Transition from Biomass Production to Lipid Accumulation in the Oleaginous Yeast Yarrowia lipolytica
journal, November 2011

Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures
journal, March 2002
  • Papanikolaou, S.; Chevalot, I.; Komaitis, M.
  • Applied Microbiology and Biotechnology, Vol. 58, Issue 3, p. 308-312
  • DOI: 10.1007/s00253-001-0897-0

Control of Lipid Accumulation in the Yeast Yarrowia lipolytica
journal, October 2008
  • Beopoulos, A.; Mrozova, Z.; Thevenieau, F.
  • Applied and Environmental Microbiology, Vol. 74, Issue 24, p. 7779-7789
  • DOI: 10.1128/AEM.01412-08