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Title: Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin

In this study, we report the atomic-scale analysis of biological interfaces using atom probe tomography. Embedding the protein ferritin in an organic polymer resin lacking nitrogen provided chemical contrast to visualize atomic distributions and distinguish organic-organic and organic-inorganic interfaces. The sample preparation method can be directly extended to further enhance the study of biological, organic and inorganic nanomaterials relevant to health, energy or the environment.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Qatar Environment and Energy Research Institute, Doha (Qatar)
Publication Date:
Report Number(s):
PNNL-SA-112512
Journal ID: ISSN 2045-2322; 48671
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; Environmental Molecular Sciences Laboratory; biomaterials - proteins; mass spectrometry; membrane structure and assembly; microscopy
OSTI Identifier:
1243203

Perea, Daniel E., Liu, Jia, Bartrand, Jonah A. G., Dicken, Quinten G., Thevuthasan, Suntharampillai Theva, Browning, Nigel D., and Evans, James E.. Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin. United States: N. p., Web. doi:10.1038/srep22321.
Perea, Daniel E., Liu, Jia, Bartrand, Jonah A. G., Dicken, Quinten G., Thevuthasan, Suntharampillai Theva, Browning, Nigel D., & Evans, James E.. Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin. United States. doi:10.1038/srep22321.
Perea, Daniel E., Liu, Jia, Bartrand, Jonah A. G., Dicken, Quinten G., Thevuthasan, Suntharampillai Theva, Browning, Nigel D., and Evans, James E.. 2016. "Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin". United States. doi:10.1038/srep22321. https://www.osti.gov/servlets/purl/1243203.
@article{osti_1243203,
title = {Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin},
author = {Perea, Daniel E. and Liu, Jia and Bartrand, Jonah A. G. and Dicken, Quinten G. and Thevuthasan, Suntharampillai Theva and Browning, Nigel D. and Evans, James E.},
abstractNote = {In this study, we report the atomic-scale analysis of biological interfaces using atom probe tomography. Embedding the protein ferritin in an organic polymer resin lacking nitrogen provided chemical contrast to visualize atomic distributions and distinguish organic-organic and organic-inorganic interfaces. The sample preparation method can be directly extended to further enhance the study of biological, organic and inorganic nanomaterials relevant to health, energy or the environment.},
doi = {10.1038/srep22321},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {2016},
month = {2}
}