In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction

Mehra-Chaudhary, Ritcha; Dai, Yumin; Sobrado, Pablo; Tanner, John J.

doi:10.1021/acs.biochem.6b00035

Title: In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction

Authors:

Mehra-Chaudhary, Ritcha ^[1]; Dai, Yumin ^[2]; Sobrado, Pablo ^[2]; Tanner, John J. ^[3]

Structural Biology Core, University of Missouri—Columbia, Columbia, Missouri 65211, United States
Department of Biochemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, Virginia 24061, United States
Departments of Biochemistry and Chemistry, University of Missouri—Columbia, Columbia, Missouri 65211, United States

Publication Date:: Tue Feb 02 00:00:00 EST 2016

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1242841

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Biochemistry

Additional Journal Information:: Journal Name: Biochemistry Journal Volume: 55 Journal Issue: 6; Journal ID: ISSN 0006-2960

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Citation Formats


                    Mehra-Chaudhary, Ritcha, Dai, Yumin, Sobrado, Pablo, and Tanner, John J. In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction.  United States: N. p., 2016. 
Web.  doi:10.1021/acs.biochem.6b00035.

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                    Mehra-Chaudhary, Ritcha, Dai, Yumin, Sobrado, Pablo, & Tanner, John J. In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction.  United States.  https://doi.org/10.1021/acs.biochem.6b00035

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                    Mehra-Chaudhary, Ritcha, Dai, Yumin, Sobrado, Pablo, and Tanner, John J. Tue .  
"In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction".  United States.  https://doi.org/10.1021/acs.biochem.6b00035.

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@article{osti_1242841,

  title        = {In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction},

  author       = {Mehra-Chaudhary, Ritcha and Dai, Yumin and Sobrado, Pablo and Tanner, John J.},

  abstractNote = {},

  doi          = {10.1021/acs.biochem.6b00035},

  journal      = {Biochemistry},

  number       = 6,

  volume       = 55,

  place        = {United States},

  year         = {Tue Feb 02 00:00:00 EST 2016},

  month        = {Tue Feb 02 00:00:00 EST 2016}

}

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Cited by: 18 works

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Works referenced in this record:

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Identification of the NAD(P)H Binding Site of Eukaryotic UDP-Galactopyranose Mutase
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X-ray Crystallography Reveals a Reduced Substrate Complex of UDP-Galactopyranose Mutase Poised for Covalent Catalysis by Flavin,
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Crystal Structures and Small-angle X-ray Scattering Analysis of UDP-galactopyranose Mutase from the Pathogenic Fungus Aspergillus fumigatus
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Dhatwalia, Richa; Singh, Harkewal; Oppenheimer, Michelle
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Characterization of the Caenorhabditis elegans UDP-galactopyranose mutase homolog glf-1 reveals an essential role for galactofuranose metabolism in nematode surface coat synthesis
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Oppenheimer, Michelle; Valenciano, Ana Lisa; Kizjakina, Karina
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Targeted Gene Deletion of Leishmania major UDP-galactopyranose Mutase Leads to Attenuated Virulence
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QM/MM Molecular Dynamics Study of the Galactopyranose → Galactofuranose Reaction Catalysed by Trypanosoma cruzi UDP-Galactopyranose Mutase
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Nucleophilic Participation of Reduced Flavin Coenzyme in Mechanism of UDP-galactopyranose Mutase*
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Structure, mechanism, and dynamics of UDP-galactopyranose mutase
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Crystal Structures of Trypanosoma cruzi UDP-Galactopyranose Mutase Implicate Flexibility of the Histidine Loop in Enzyme Activation
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Dhatwalia, Richa; Singh, Harkewal; Oppenheimer, Michelle
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X-ray Crystallography Reveals a Reduced Substrate Complex of UDP-Galactopyranose Mutase Poised for Covalent Catalysis by Flavin

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Title: In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction

Citation Formats

Cell Wall Core Galactofuran Synthesis Is Essential for Growth of Mycobacteria journal, July 2001

Contribution of Galactofuranose to the Virulence of the Opportunistic Pathogen Aspergillus fumigatus journal, June 2008

UDP-Galactopyranose Mutase in Nematodes journal, June 2013

Identification of the NAD(P)H Binding Site of Eukaryotic UDP-Galactopyranose Mutase journal, October 2012

Genes required for mycobacterial growth defined by high density mutagenesis: Genes required for mycobacterial growth journal, March 2003

Eukaryotic UDP-Galactopyranose Mutase ( GLF Gene) in Microbial and Metazoal Pathogens journal, June 2005

A unique catalytic mechanism for UDP-galactopyranose mutase journal, May 2004

X-ray Crystallography Reveals a Reduced Substrate Complex of UDP-Galactopyranose Mutase Poised for Covalent Catalysis by Flavin, journal, October 2009

Galactofuranose in eukaryotes: aspects of biosynthesis and functional impact journal, September 2011

Crystal Structures and Small-angle X-ray Scattering Analysis of UDP-galactopyranose Mutase from the Pathogenic Fungus Aspergillus fumigatus journal, January 2012

Characterization of the Caenorhabditis elegans UDP-galactopyranose mutase homolog glf-1 reveals an essential role for galactofuranose metabolism in nematode surface coat synthesis journal, November 2009

Chemical Mechanism of UDP-Galactopyranose Mutase from Trypanosoma cruzi: A Potential Drug Target against Chagas' Disease journal, March 2012

Targeted Gene Deletion of Leishmania major UDP-galactopyranose Mutase Leads to Attenuated Virulence journal, April 2007

QM/MM Molecular Dynamics Study of the Galactopyranose → Galactofuranose Reaction Catalysed by Trypanosoma cruzi UDP-Galactopyranose Mutase journal, October 2014

Nucleophilic Participation of Reduced Flavin Coenzyme in Mechanism of UDP-galactopyranose Mutase* journal, February 2012

Trypanosoma cruzi Uses a 45-kDa Mucin for Adhesion to Mammalian Cells journal, January 2002

Tautomerization in the UDP-Galactopyranose Mutase Mechanism: A DFT-Cluster and QM/MM Investigation journal, November 2012

Structure, mechanism, and dynamics of UDP-galactopyranose mutase journal, February 2014

Crystal Structures of Trypanosoma cruzi UDP-Galactopyranose Mutase Implicate Flexibility of the Histidine Loop in Enzyme Activation journal, June 2012

Cell Wall Core Galactofuran Synthesis Is Essential for Growth of Mycobacteria
journal, July 2001

Contribution of Galactofuranose to the Virulence of the Opportunistic Pathogen Aspergillus fumigatus
journal, June 2008

UDP-Galactopyranose Mutase in Nematodes
journal, June 2013

Identification of the NAD(P)H Binding Site of Eukaryotic UDP-Galactopyranose Mutase
journal, October 2012

Genes required for mycobacterial growth defined by high density mutagenesis: Genes required for mycobacterial growth
journal, March 2003

Eukaryotic UDP-Galactopyranose Mutase ( GLF Gene) in Microbial and Metazoal Pathogens
journal, June 2005

A unique catalytic mechanism for UDP-galactopyranose mutase
journal, May 2004

X-ray Crystallography Reveals a Reduced Substrate Complex of UDP-Galactopyranose Mutase Poised for Covalent Catalysis by Flavin,
journal, October 2009

Galactofuranose in eukaryotes: aspects of biosynthesis and functional impact
journal, September 2011

Crystal Structures and Small-angle X-ray Scattering Analysis of UDP-galactopyranose Mutase from the Pathogenic Fungus Aspergillus fumigatus
journal, January 2012

Characterization of the Caenorhabditis elegans UDP-galactopyranose mutase homolog glf-1 reveals an essential role for galactofuranose metabolism in nematode surface coat synthesis
journal, November 2009

Chemical Mechanism of UDP-Galactopyranose Mutase from Trypanosoma cruzi: A Potential Drug Target against Chagas' Disease
journal, March 2012

Targeted Gene Deletion of Leishmania major UDP-galactopyranose Mutase Leads to Attenuated Virulence
journal, April 2007

QM/MM Molecular Dynamics Study of the Galactopyranose → Galactofuranose Reaction Catalysed by Trypanosoma cruzi UDP-Galactopyranose Mutase
journal, October 2014

Nucleophilic Participation of Reduced Flavin Coenzyme in Mechanism of UDP-galactopyranose Mutase*
journal, February 2012

Trypanosoma cruzi Uses a 45-kDa Mucin for Adhesion to Mammalian Cells
journal, January 2002

Tautomerization in the UDP-Galactopyranose Mutase Mechanism: A DFT-Cluster and QM/MM Investigation
journal, November 2012

Structure, mechanism, and dynamics of UDP-galactopyranose mutase
journal, February 2014

Crystal Structures of Trypanosoma cruzi UDP-Galactopyranose Mutase Implicate Flexibility of the Histidine Loop in Enzyme Activation
journal, June 2012