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Title: Analysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood

Journal Article · · PLoS Genetics
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  1. Univ. of Tokyo (Japan)
  2. Univ. of Toronto, ON (Canada)
  3. Univ. of Zaragoza (Spain)
  4. Spanish National Research Council (CSIC), Madrid (Spain)
  5. Univ. of Minnesota, St. Paul, MN (United States)
  6. Pontifical Catholic University of Chile, Santiago (Chile)
  7. Austrian Inst. of Technology GmbH, Tulin (Austria)
  8. Vienna Univ. of Technology, Vienna (Austria)
  9. USDA, Madison, WI (United States)
  10. Univ. of Wisconsin Biotechnology Center, Madison, WI (United States)
  11. Univ. of Cincinnati, OH (United States)
  12. Univ. of Helsinki (Finland)
  13. Friedrich-Schiller-Univ., Jena (Germany)
  14. IRNAS, Seville (Spain)
  15. U.S. Joint Genome Inst., Walnut Creek, CA (United States)
  16. Aix-Marseille Univ., and CNRS/IN2P3, Marseille (France)
  17. Georg-August Univ., Gottingen (Germany)
  18. Novozymes, Inc., Davis, CA (United States)
  19. Univ. of Georgia, Athens, GA (United States)
  20. Univ. of North Carolina, Chapel Hill, NC (United States)
+ Show Author Affiliations

Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset of these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high content of extractives, which retards the establishment of other fungal species. The mechanism(s) by which P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report the annotated P. gigantea genome and compare profiles of its transcriptome and secretome when cultured on freshcut versus solvent-extracted loblolly pine wood. The P. gigantea genome contains a conventional repertoire of hydrolase genes involved in cellulose/hemicellulose degradation, whose patterns of expression were relatively unperturbed by the absence of extractives. The expression of genes typically ascribed to lignin degradation was also largely unaffected. In contrast, genes likely involved in the transformation and detoxification of wood extractives were highly induced in its presence. Their products included an ABC transporter, lipases, cytochrome P450s, glutathione S-transferase and aldehyde dehydrogenase. Other regulated genes of unknown function and several constitutively expressed genes are also likely involved in P. gigantea’s extractives metabolism. These results contribute to our fundamental understanding of pioneer colonization of conifer wood and provide insight into the diverse chemistries employed by fungi in carbon cycling processes.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Genomics Division
Sponsoring Organization:
USDOE Office of Science (SC). Joint Genome Institute; USDA
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1242697
Report Number(s):
LBNL--177889; ir:177889
Journal Information:
PLoS Genetics, Journal Name: PLoS Genetics Journal Issue: 12 Vol. 10; ISSN 1553-7404
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (2)

Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution journal October 2017
A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus journal February 2016

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