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Title: The Fate of Marine Bacterial Exopolysaccharide in Natural Marine Microbial Communities

Abstract

Most marine bacteria produce exopolysaccharides (EPS), and bacterial EPS represent an important source of dissolved organic carbon in marine ecosystems. It was proposed that bacterial EPS rich in uronic acid is resistant to mineralization by microbes and thus has a long residence time in global oceans. To confirm this hypothesis, bacterial EPS rich in galacturonic acid was isolated from Alteromonas sp. JL2810. The EPS was used to amend natural seawater to investigate the bioavailability of this EPS by native populations, in the presence and absence of ammonium and phosphate amendment. The data indicated that the bacterial EPS could not be completely consumed during the cultivation period and that the bioavailability of EPS was not only determined by its intrinsic properties, but was also determined by other factors such as the availability of inorganic nutrients. During the experiment, the humic-like component of fluorescent dissolved organic matter (FDOM) was freshly produced. Bacterial community structure analysis indicated that the class Flavobacteria of the phylum Bacteroidetes was the major contributor for the utilization of EPS. This report is the first to indicate that Flavobacteria are a major contributor to bacterial EPS degradation. Finally, the fraction of EPS that could not be completely utilized andmore » the FDOM (e.g., humic acid-like substances) produced de novo may be refractory and may contribute to the carbon storage in the oceans.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Xiamen Univ., Xiamen, Fujian (China). State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres
Publication Date:
Research Org.:
Xiamen Univ., Xiamen, Fujian (China). State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1241138
Grant/Contract Number:  
FG02-93ER20097
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 10; Journal Issue: 11; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhang, Zilian, Chen, Yi, Wang, Rui, Cai, Ruanhong, Fu, Yingnan, Jiao, Nianzhi, and Quigg, Antonietta. The Fate of Marine Bacterial Exopolysaccharide in Natural Marine Microbial Communities. United States: N. p., 2015. Web. doi:10.1371/journal.pone.0142690.
Zhang, Zilian, Chen, Yi, Wang, Rui, Cai, Ruanhong, Fu, Yingnan, Jiao, Nianzhi, & Quigg, Antonietta. The Fate of Marine Bacterial Exopolysaccharide in Natural Marine Microbial Communities. United States. doi:10.1371/journal.pone.0142690.
Zhang, Zilian, Chen, Yi, Wang, Rui, Cai, Ruanhong, Fu, Yingnan, Jiao, Nianzhi, and Quigg, Antonietta. Mon . "The Fate of Marine Bacterial Exopolysaccharide in Natural Marine Microbial Communities". United States. doi:10.1371/journal.pone.0142690. https://www.osti.gov/servlets/purl/1241138.
@article{osti_1241138,
title = {The Fate of Marine Bacterial Exopolysaccharide in Natural Marine Microbial Communities},
author = {Zhang, Zilian and Chen, Yi and Wang, Rui and Cai, Ruanhong and Fu, Yingnan and Jiao, Nianzhi and Quigg, Antonietta},
abstractNote = {Most marine bacteria produce exopolysaccharides (EPS), and bacterial EPS represent an important source of dissolved organic carbon in marine ecosystems. It was proposed that bacterial EPS rich in uronic acid is resistant to mineralization by microbes and thus has a long residence time in global oceans. To confirm this hypothesis, bacterial EPS rich in galacturonic acid was isolated from Alteromonas sp. JL2810. The EPS was used to amend natural seawater to investigate the bioavailability of this EPS by native populations, in the presence and absence of ammonium and phosphate amendment. The data indicated that the bacterial EPS could not be completely consumed during the cultivation period and that the bioavailability of EPS was not only determined by its intrinsic properties, but was also determined by other factors such as the availability of inorganic nutrients. During the experiment, the humic-like component of fluorescent dissolved organic matter (FDOM) was freshly produced. Bacterial community structure analysis indicated that the class Flavobacteria of the phylum Bacteroidetes was the major contributor for the utilization of EPS. This report is the first to indicate that Flavobacteria are a major contributor to bacterial EPS degradation. Finally, the fraction of EPS that could not be completely utilized and the FDOM (e.g., humic acid-like substances) produced de novo may be refractory and may contribute to the carbon storage in the oceans.},
doi = {10.1371/journal.pone.0142690},
journal = {PLoS ONE},
number = 11,
volume = 10,
place = {United States},
year = {2015},
month = {11}
}

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