Quantitative metrics for assessment of chemical image quality and spatial resolution

doi:10.1002/rcm.7519

Title: Quantitative metrics for assessment of chemical image quality and spatial resolution

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Rationale: Currently objective/quantitative descriptions of the quality and spatial resolution of mass spectrometry derived chemical images are not standardized. Development of these standardized metrics is required to objectively describe chemical imaging capabilities of existing and/or new mass spectrometry imaging technologies. Such metrics would allow unbiased judgment of intra-laboratory advancement and/or inter-laboratory comparison for these technologies if used together with standardized surfaces. Methods: We developed two image metrics, viz., chemical image contrast (ChemIC) based on signal-to-noise related statistical measures on chemical image pixels and corrected resolving power factor (cRPF) constructed from statistical analysis of mass-to-charge chronograms across features of interest in an image. These metrics, quantifying chemical image quality and spatial resolution, respectively, were used to evaluate chemical images of a model photoresist patterned surface collected using a laser ablation/liquid vortex capture mass spectrometry imaging system under different instrument operational parameters. Results: The calculated ChemIC and cRPF metrics determined in an unbiased fashion the relative ranking of chemical image quality obtained with the laser ablation/liquid vortex capture mass spectrometry imaging system. These rankings were used to show that both chemical image contrast and spatial resolution deteriorated with increasing surface scan speed, increased lane spacing and decreasing size of surface features. Conclusions:more »

Authors:

Kertesz, Vilmos ^[1] ; Cahill, John F. ^[1] ; Van Berkel, Gary J. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division

Publication Date:: 2016-02-28

Grant/Contract Number:: AC05-00OR22725

Type:: Accepted Manuscript

Journal Name:: Rapid Communications in Mass Spectrometry

Additional Journal Information:: Journal Volume: 30; Journal Issue: 7; Journal ID: ISSN 0951-4198

Publisher:: Wiley

Research Org:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org:: USDOE Office of Science (SC)

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

OSTI Identifier:: 1240566


                    Kertesz, Vilmos, Cahill, John F., and Van Berkel, Gary J.. Quantitative metrics for assessment of chemical image quality and spatial resolution.  United States: N. p.,  
        Web.  doi:10.1002/rcm.7519.

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                    Kertesz, Vilmos, Cahill, John F., & Van Berkel, Gary J.. Quantitative metrics for assessment of chemical image quality and spatial resolution.  United States.  doi:10.1002/rcm.7519.

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                    Kertesz, Vilmos, Cahill, John F., and Van Berkel, Gary J.. 2016.  
        "Quantitative metrics for assessment of chemical image quality and spatial resolution".  United States.  
        doi:10.1002/rcm.7519.  https://www.osti.gov/servlets/purl/1240566.

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@article{osti_1240566,

  title        = {Quantitative metrics for assessment of chemical image quality and spatial resolution},

  author       = {Kertesz, Vilmos and Cahill, John F. and Van Berkel, Gary J.},

  abstractNote = {Rationale: Currently objective/quantitative descriptions of the quality and spatial resolution of mass spectrometry derived chemical images are not standardized. Development of these standardized metrics is required to objectively describe chemical imaging capabilities of existing and/or new mass spectrometry imaging technologies. Such metrics would allow unbiased judgment of intra-laboratory advancement and/or inter-laboratory comparison for these technologies if used together with standardized surfaces. Methods: We developed two image metrics, viz., chemical image contrast (ChemIC) based on signal-to-noise related statistical measures on chemical image pixels and corrected resolving power factor (cRPF) constructed from statistical analysis of mass-to-charge chronograms across features of interest in an image. These metrics, quantifying chemical image quality and spatial resolution, respectively, were used to evaluate chemical images of a model photoresist patterned surface collected using a laser ablation/liquid vortex capture mass spectrometry imaging system under different instrument operational parameters. Results: The calculated ChemIC and cRPF metrics determined in an unbiased fashion the relative ranking of chemical image quality obtained with the laser ablation/liquid vortex capture mass spectrometry imaging system. These rankings were used to show that both chemical image contrast and spatial resolution deteriorated with increasing surface scan speed, increased lane spacing and decreasing size of surface features. Conclusions: ChemIC and cRPF, respectively, were developed and successfully applied for the objective description of chemical image quality and spatial resolution of chemical images collected from model surfaces using a laser ablation/liquid vortex capture mass spectrometry imaging system.},

  doi          = {10.1002/rcm.7519},

  journal      = {Rapid Communications in Mass Spectrometry},

  number       = 7,

  volume       = 30,

  place        = {United States},

  year         = {2016},

  month        = {2}

}

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10.1002/rcm.7519
https://doi.org/10.1002/rcm.7519

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