REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to establish the size of the chloroplast compartment
Abstract
Eukaryotic cells require mechanisms to establish the proportion of cellular volume devoted to particular organelles. These mechanisms are poorly understood. From a screen for plastid-to-nucleus signaling mutants in Arabidopsis thaliana, we cloned a mutant allele of a gene that encodes a protein of unknown function that is homologous to two other Arabidopsis genes of unknown function and Arabidopsis. In contrast to FRIENDLY, these three homologs of FRIENDLY are found only in photosynthetic organisms. Based on these data, we proposed that FRIENDLY expanded into a small gene family to help regulate the energy metabolism of cells that contain both mitochondria and chloroplasts. Indeed, we found that knocking out these genes caused a number of chloroplast phenotypes, including a reduction in the proportion of cellular volume devoted to chloroplasts to 50% of wild type. Thus, we refer to these genes as REDUCED CHLOROPLAST COVERAGE (REC). The size of the chloroplast compartment was reduced most in rec1 mutants. The REC1 protein accumulated in the cytosol and the nucleus. REC1 was excluded from the nucleus when plants were treated with amitrole, which inhibits cell expansion and chloroplast function. Finally, we conclude that REC1 is an extraplastidic protein that helps to establish the size ofmore »
- Authors:
-
- Department of Plant Biology, Michigan State University, East Lansing, MI 48824,, Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824,, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824
- Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824,
- Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824,, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824
- Department of Plant Biology, Michigan State University, East Lansing, MI 48824,, Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824,
- Department of Plant Biology, Michigan State University, East Lansing, MI 48824,
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1237516
- Alternate Identifier(s):
- OSTI ID: 1348392
- Grant/Contract Number:
- FG02-91ER20021; FG02-06ER15808; NNX12AN71G; MCB-1121943; MCB1243792; IOB 0517841; IOS 1021755
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 113 Journal Issue: 8; Journal ID: ISSN 0027-8424
- Publisher:
- Proceedings of the National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Arabidopsis; chlorophyll; chloroplast; chloroplast coverage; plastid signaling
Citation Formats
Larkin, Robert M., Stefano, Giovanni, Ruckle, Michael E., Stavoe, Andrea K., Sinkler, Christopher A., Brandizzi, Federica, Malmstrom, Carolyn M., and Osteryoung, Katherine W. REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to establish the size of the chloroplast compartment. United States: N. p., 2016.
Web. doi:10.1073/pnas.1515741113.
Larkin, Robert M., Stefano, Giovanni, Ruckle, Michael E., Stavoe, Andrea K., Sinkler, Christopher A., Brandizzi, Federica, Malmstrom, Carolyn M., & Osteryoung, Katherine W. REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to establish the size of the chloroplast compartment. United States. https://doi.org/10.1073/pnas.1515741113
Larkin, Robert M., Stefano, Giovanni, Ruckle, Michael E., Stavoe, Andrea K., Sinkler, Christopher A., Brandizzi, Federica, Malmstrom, Carolyn M., and Osteryoung, Katherine W. Tue .
"REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to establish the size of the chloroplast compartment". United States. https://doi.org/10.1073/pnas.1515741113.
@article{osti_1237516,
title = {REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to establish the size of the chloroplast compartment},
author = {Larkin, Robert M. and Stefano, Giovanni and Ruckle, Michael E. and Stavoe, Andrea K. and Sinkler, Christopher A. and Brandizzi, Federica and Malmstrom, Carolyn M. and Osteryoung, Katherine W.},
abstractNote = {Eukaryotic cells require mechanisms to establish the proportion of cellular volume devoted to particular organelles. These mechanisms are poorly understood. From a screen for plastid-to-nucleus signaling mutants in Arabidopsis thaliana, we cloned a mutant allele of a gene that encodes a protein of unknown function that is homologous to two other Arabidopsis genes of unknown function and Arabidopsis. In contrast to FRIENDLY, these three homologs of FRIENDLY are found only in photosynthetic organisms. Based on these data, we proposed that FRIENDLY expanded into a small gene family to help regulate the energy metabolism of cells that contain both mitochondria and chloroplasts. Indeed, we found that knocking out these genes caused a number of chloroplast phenotypes, including a reduction in the proportion of cellular volume devoted to chloroplasts to 50% of wild type. Thus, we refer to these genes as REDUCED CHLOROPLAST COVERAGE (REC). The size of the chloroplast compartment was reduced most in rec1 mutants. The REC1 protein accumulated in the cytosol and the nucleus. REC1 was excluded from the nucleus when plants were treated with amitrole, which inhibits cell expansion and chloroplast function. Finally, we conclude that REC1 is an extraplastidic protein that helps to establish the size of the chloroplast compartment, and that signals derived from cell expansion or chloroplasts may regulate REC1.},
doi = {10.1073/pnas.1515741113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 8,
volume = 113,
place = {United States},
year = {Tue Feb 09 00:00:00 EST 2016},
month = {Tue Feb 09 00:00:00 EST 2016}
}
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https://doi.org/10.1073/pnas.1515741113
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