A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival
Abstract
Yersinia enterocolitica is typically considered an extracellular pathogen; however, during the course of an infection, a significant number of bacteria are stably maintained within host cell vacuoles. Little is known about this population and the role it plays during an infection. To address this question and to elucidate the spatially and temporally dynamic gene expression patterns of Y. enterocoliticabiovar 1B through the course of an in vitro infection, transcriptome sequencing and differential gene expression analysis of bacteria infecting murine macrophage cells were performed under four distinct conditions. Bacteria were first grown in a nutrient-rich medium at 26°C to establish a baseline of gene expression that is unrelated to infection. The transcriptomes of these bacteria were then compared to bacteria grown in a conditioned cell culture medium at 37°C to identify genes that were differentially expressed in response to the increased temperature and medium but not in response to host cells. Infections were then performed, and the transcriptomes of bacteria found on the extracellular surface and intracellular compartments were analyzed individually. The upregulated genes revealed potential roles for a variety of systems in promoting intracellular virulence, including the Ysa type III secretion system, the Yts2 type II secretion system, and themore »
- Authors:
-
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Univ. of California, Davis, CA (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1235333
- Report Number(s):
- SAND-2014-3732J
Journal ID: ISSN 0019-9567; 516866
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Infection and Immunity
- Additional Journal Information:
- Journal Volume: 83; Journal Issue: 7; Journal ID: ISSN 0019-9567
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bent, Zachary W., Poorey, Kunal, Brazel, David M., LaBauve, Annette E., Sinha, Anupama, Curtis, Deanna Joy, House, Samantha E., Tew, Karen E., Hamblin, Rachelle Y., Williams, Kelly Porter, Branda, Steven S., Young, Glenn M., and Meagher, Robert J. A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival. United States: N. p., 2015.
Web. doi:10.1128/IAI.02922-14.
Bent, Zachary W., Poorey, Kunal, Brazel, David M., LaBauve, Annette E., Sinha, Anupama, Curtis, Deanna Joy, House, Samantha E., Tew, Karen E., Hamblin, Rachelle Y., Williams, Kelly Porter, Branda, Steven S., Young, Glenn M., & Meagher, Robert J. A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival. United States. https://doi.org/10.1128/IAI.02922-14
Bent, Zachary W., Poorey, Kunal, Brazel, David M., LaBauve, Annette E., Sinha, Anupama, Curtis, Deanna Joy, House, Samantha E., Tew, Karen E., Hamblin, Rachelle Y., Williams, Kelly Porter, Branda, Steven S., Young, Glenn M., and Meagher, Robert J. Mon .
"A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival". United States. https://doi.org/10.1128/IAI.02922-14. https://www.osti.gov/servlets/purl/1235333.
@article{osti_1235333,
title = {A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival},
author = {Bent, Zachary W. and Poorey, Kunal and Brazel, David M. and LaBauve, Annette E. and Sinha, Anupama and Curtis, Deanna Joy and House, Samantha E. and Tew, Karen E. and Hamblin, Rachelle Y. and Williams, Kelly Porter and Branda, Steven S. and Young, Glenn M. and Meagher, Robert J.},
abstractNote = {Yersinia enterocolitica is typically considered an extracellular pathogen; however, during the course of an infection, a significant number of bacteria are stably maintained within host cell vacuoles. Little is known about this population and the role it plays during an infection. To address this question and to elucidate the spatially and temporally dynamic gene expression patterns of Y. enterocoliticabiovar 1B through the course of an in vitro infection, transcriptome sequencing and differential gene expression analysis of bacteria infecting murine macrophage cells were performed under four distinct conditions. Bacteria were first grown in a nutrient-rich medium at 26°C to establish a baseline of gene expression that is unrelated to infection. The transcriptomes of these bacteria were then compared to bacteria grown in a conditioned cell culture medium at 37°C to identify genes that were differentially expressed in response to the increased temperature and medium but not in response to host cells. Infections were then performed, and the transcriptomes of bacteria found on the extracellular surface and intracellular compartments were analyzed individually. The upregulated genes revealed potential roles for a variety of systems in promoting intracellular virulence, including the Ysa type III secretion system, the Yts2 type II secretion system, and the Tad pilus. It was further determined that mutants of each of these systems had decreased virulence while infecting macrophages. Overall, these results reveal the complete set of genes expressed by Y. enterocolitica in response to infection and provide the groundwork for future virulence studies.},
doi = {10.1128/IAI.02922-14},
journal = {Infection and Immunity},
number = 7,
volume = 83,
place = {United States},
year = {Mon Apr 20 00:00:00 EDT 2015},
month = {Mon Apr 20 00:00:00 EDT 2015}
}
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