Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C
Abstract
Mutations in the gene for the lysosomal enzyme glucocerebrosidase (GCase) cause Gaucher disease and are the most common risk factor for Parkinson disease (PD). Analytical ultracentrifugation of 8 μM GCase shows equilibrium between monomer and dimer forms. However, in the presence of its co-factor saposin C (Sap C), only monomer GCase is seen. Isothermal calorimetry confirms that Sap C associates with GCase in solution in a 1:1 complex (Kd = 2.1 ± 1.1 μM). Saturation cross-transfer NMR determined that the region of Sap C contacting GCase includes residues 63–66 and 74–76, which is distinct from the region known to enhance GCase activity. Because α-synuclein (α-syn), a protein closely associated with PD etiology, competes with Sap C for GCase binding, its interaction with GCase was also measured by ultracentrifugation and saturation cross-transfer. Unlike Sap C, binding of α-syn to GCase does not affect multimerization. However, adding α-syn reduces saturation cross-transfer from Sap C to GCase, confirming displacement. To explore where Sap C might disrupt multimeric GCase, GCase x-ray structures were analyzed using the program PISA, which predicted stable dimer and tetramer forms. In conclusion, for the most frequently predicted multimer interface, the GCase active sites are partially buried, suggesting that Sapmore »
- Authors:
-
- National Inst. of Health (NIH), Bethesda, MD (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH)
- OSTI Identifier:
- 1225745
- Alternate Identifier(s):
- OSTI ID: 1233917
- Grant/Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 457; Journal Issue: 4; Journal ID: ISSN 0006-291X
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Gaucher disease; Parkinson disease; NMR; AUC; α-synuclein
Citation Formats
Gruschus, James M., Jiang, Zhiping, Yap, Thai Leong, Hill, Stephanie A., Grishaev, Alexander, Piszczek, Grzegorz, Sidransky, Ellen, and Lee, Jennifer C. Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C. United States: N. p., 2015.
Web. doi:10.1016/j.bbrc.2015.01.024.
Gruschus, James M., Jiang, Zhiping, Yap, Thai Leong, Hill, Stephanie A., Grishaev, Alexander, Piszczek, Grzegorz, Sidransky, Ellen, & Lee, Jennifer C. Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C. United States. https://doi.org/10.1016/j.bbrc.2015.01.024
Gruschus, James M., Jiang, Zhiping, Yap, Thai Leong, Hill, Stephanie A., Grishaev, Alexander, Piszczek, Grzegorz, Sidransky, Ellen, and Lee, Jennifer C. Fri .
"Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C". United States. https://doi.org/10.1016/j.bbrc.2015.01.024. https://www.osti.gov/servlets/purl/1225745.
@article{osti_1225745,
title = {Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C},
author = {Gruschus, James M. and Jiang, Zhiping and Yap, Thai Leong and Hill, Stephanie A. and Grishaev, Alexander and Piszczek, Grzegorz and Sidransky, Ellen and Lee, Jennifer C.},
abstractNote = {Mutations in the gene for the lysosomal enzyme glucocerebrosidase (GCase) cause Gaucher disease and are the most common risk factor for Parkinson disease (PD). Analytical ultracentrifugation of 8 μM GCase shows equilibrium between monomer and dimer forms. However, in the presence of its co-factor saposin C (Sap C), only monomer GCase is seen. Isothermal calorimetry confirms that Sap C associates with GCase in solution in a 1:1 complex (Kd = 2.1 ± 1.1 μM). Saturation cross-transfer NMR determined that the region of Sap C contacting GCase includes residues 63–66 and 74–76, which is distinct from the region known to enhance GCase activity. Because α-synuclein (α-syn), a protein closely associated with PD etiology, competes with Sap C for GCase binding, its interaction with GCase was also measured by ultracentrifugation and saturation cross-transfer. Unlike Sap C, binding of α-syn to GCase does not affect multimerization. However, adding α-syn reduces saturation cross-transfer from Sap C to GCase, confirming displacement. To explore where Sap C might disrupt multimeric GCase, GCase x-ray structures were analyzed using the program PISA, which predicted stable dimer and tetramer forms. In conclusion, for the most frequently predicted multimer interface, the GCase active sites are partially buried, suggesting that Sap C might disrupt the multimer by binding near the active site.},
doi = {10.1016/j.bbrc.2015.01.024},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 457,
place = {United States},
year = {Fri Jan 16 00:00:00 EST 2015},
month = {Fri Jan 16 00:00:00 EST 2015}
}
Web of Science
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