Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Mueller, C.; Marx, A.; Epp, S. W.; Zhong, Y.; Kuo, A.; Balo, A. R.; Soman, J.; Schotte, F.; Lemke, H. T.; Owen, R. L.; Pai, E. F.; Pearson, A. R.; Olson, J. S.; Anfinrud, P. A.; Ernst, O. P.; Miller, R. J. Dwayne

doi:10.1063/1.4928706

Title: Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Journal Article · Tue Aug 18 04:00:00 UTC 2015 · Structural Dynamics

DOI: https://doi.org/10.1063/1.4928706 · OSTI ID:1230061

Mueller, C. ^[1]; Marx, A. ^[2]; Epp, S. W. ^[2]; Zhong, Y. ^[2]; Kuo, A. ^[1]; Balo, A. R. ^[1]; Soman, J. ^[3]; Schotte, F. ^[4]; Lemke, H. T. ^[5]; Owen, R. L. ^[6]; Pai, E. F. ^[7]; Pearson, A. R. ^[8]; Olson, J. S. ^[3]; Anfinrud, P. A. ^[4]; Ernst, O. P. ^[1]; Miller, R. J. Dwayne ^[9]

Univ. of Toronto, Toronto, ON (Canada)
Max Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
Rice Univ., Houston, TX (United States)
National Institutes of Health, Bethesda, MD (United States)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Diamond Light Source, Didcot (United Kingdom)
Univ. of Toronto, Toronto, ON (Canada); Univ. of Toronto and Campbell Family Institute for Cancer Research, Univ. Health Network, Toronto, ON (Canada)
Univ. of Hamburg, Hamburg (Germany)
Univ. of Toronto, Toronto, ON (Canada); Max Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)

We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources. Compared to other in situ approaches, we observe extremely low background and high diffraction data quality. The chip design is robust and allows fast and efficient loading of thousands of small crystals. The ability to load a large number of protein crystals, at room temperature and with high efficiency, into prescribed positions enables high throughput automated serial crystallography with microfocus synchrotron beamlines. In addition, we demonstrate the application of this chip for femtosecond time-resolved serial crystallography at the Linac Coherent Light Source (LCLS, Menlo Park, California, USA). As a result, the chip concept enables multiple images to be acquired from each crystal, allowing differential detection of changes in diffraction intensities in order to obtain high signal-to-noise and fully exploit the time resolution capabilities of XFELs.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC03-76SF00515

OSTI ID:: 1230061

Journal Information:: Structural Dynamics, Journal Name: Structural Dynamics Journal Issue: 5 Vol. 2; ISSN SDTYAE; ISSN 2329-7778

Publisher:: American Crystallographic Association/AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Sample Delivery Media for Serial Crystallography Nam, Ki International Journal of Molecular Sciences, Vol. 20, Issue 5 https://doi.org/10.3390/ijms20051094	journal	March 2019

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