Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture
Abstract
A commercial apple pectin was sequentially digested with the cloned enzymes endopolygalacturonase, galactanase, arabinofuranosidase, xylogalacturonase, and rhamnogalacturonan hydrolase. The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, anion exchange, and size-exclusion chromatography. Fractions from the ion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOF MS. An oligosaccharide (RGP14P3) was identified and its structure, α -D-Gal p A- - α -L-Rha p - - α -D-Gal p A- - α -L-Rha p - - α -D-Gal p A, determined by 1D and 2D NMR spectrometry. This oligosaccharide probably represents a direct connection between homogalacturonan and rhamnogalacturonan in pectin. Alternatively, it could indicate that the nonreducing end of rhamnogalacturonan starts with a galacturonic acid residue.
- Authors:
-
- Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078-3035, USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1227787
- Grant/Contract Number:
- FG02-96ER20215
- Resource Type:
- Published Article
- Journal Name:
- International Journal of Carbohydrate Chemistry
- Additional Journal Information:
- Journal Name: International Journal of Carbohydrate Chemistry Journal Volume: 2014; Journal ID: ISSN 1687-9341
- Publisher:
- Hindawi Publishing Corporation
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Citation Formats
Wu, Xiangmei, and Mort, Andrew. Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture. Country unknown/Code not available: N. p., 2014.
Web. doi:10.1155/2014/347381.
Wu, Xiangmei, & Mort, Andrew. Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture. Country unknown/Code not available. https://doi.org/10.1155/2014/347381
Wu, Xiangmei, and Mort, Andrew. Sun .
"Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture". Country unknown/Code not available. https://doi.org/10.1155/2014/347381.
@article{osti_1227787,
title = {Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture},
author = {Wu, Xiangmei and Mort, Andrew},
abstractNote = {A commercial apple pectin was sequentially digested with the cloned enzymes endopolygalacturonase, galactanase, arabinofuranosidase, xylogalacturonase, and rhamnogalacturonan hydrolase. The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, anion exchange, and size-exclusion chromatography. Fractions from the ion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOF MS. An oligosaccharide (RGP14P3) was identified and its structure, α -D-Gal p A- ( 1 → 2 ) - α -L-Rha p - ( 1 → 4 ) - α -D-Gal p A- ( 1 → 2 ) - α -L-Rha p - ( 1 → 4 ) - α -D-Gal p A, determined by 1D and 2D NMR spectrometry. This oligosaccharide probably represents a direct connection between homogalacturonan and rhamnogalacturonan in pectin. Alternatively, it could indicate that the nonreducing end of rhamnogalacturonan starts with a galacturonic acid residue.},
doi = {10.1155/2014/347381},
journal = {International Journal of Carbohydrate Chemistry},
number = ,
volume = 2014,
place = {Country unknown/Code not available},
year = {Sun Dec 14 00:00:00 EST 2014},
month = {Sun Dec 14 00:00:00 EST 2014}
}
https://doi.org/10.1155/2014/347381
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