Immunoglobulin Gene Rearrangement Can Precede Gene Rearrangement
Abstract
Immunoglobulin genes are generated during differentiation of B lymphocytes by joining gene segments. A mouse pre-B cell contains a functional immunoglobulin heavy-chain gene, but no light-chain gene. Although there is only one heavy-chain locus, there are two lightchain loci: and .It has been reported that loci in the germ-line configuration are never (in man) or very rarely (in the mouse) present in cells with functionally rearranged -chain genes. Two explanations have been proposed to explain this: (a) the ordered rearrangement theory, which postulates that light-chain gene rearrangement in the pre-B cell is first attempted at the locus, and that only upon failure to produce a functional chain is there an attempt to rearrange the locus; and (b) the stochastic theory, which postulates that rearrangement at the locus proceeds at a rate that is intrinsically much slower than that at the locus. We show here that -chain genes are generated whether or not the locus has lost its germ-line arrangement, a result that is compatible only with the stochastic theory.
- Authors:
-
- Department of Microbiology and Immulology, University of California, San Francisco, California 94143-0414, USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1227786
- Grant/Contract Number:
- AC03-76-SF01012
- Resource Type:
- Published Article
- Journal Name:
- Developmental Immunology
- Additional Journal Information:
- Journal Name: Developmental Immunology Journal Volume: 1 Journal Issue: 1; Journal ID: ISSN 1044-6672
- Publisher:
- Hindawi Publishing Corporation
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Citation Formats
Berg, Jörg, Mcdowell, Mindy, Jäck, Hans-Martin, and Wabl, Matthias. Immunoglobulin λ Gene Rearrangement Can Precede κ Gene Rearrangement. Country unknown/Code not available: N. p., 1990.
Web. doi:10.1155/1990/56014.
Berg, Jörg, Mcdowell, Mindy, Jäck, Hans-Martin, & Wabl, Matthias. Immunoglobulin λ Gene Rearrangement Can Precede κ Gene Rearrangement. Country unknown/Code not available. https://doi.org/10.1155/1990/56014
Berg, Jörg, Mcdowell, Mindy, Jäck, Hans-Martin, and Wabl, Matthias. Mon .
"Immunoglobulin λ Gene Rearrangement Can Precede κ Gene Rearrangement". Country unknown/Code not available. https://doi.org/10.1155/1990/56014.
@article{osti_1227786,
title = {Immunoglobulin λ Gene Rearrangement Can Precede κ Gene Rearrangement},
author = {Berg, Jörg and Mcdowell, Mindy and Jäck, Hans-Martin and Wabl, Matthias},
abstractNote = {Immunoglobulin genes are generated during differentiation of B lymphocytes by joining gene segments. A mouse pre-B cell contains a functional immunoglobulin heavy-chain gene, but no light-chain gene. Although there is only one heavy-chain locus, there are two lightchain loci: κ and λ .It has been reported that κ loci in the germ-line configuration are never (in man) or very rarely (in the mouse) present in cells with functionally rearranged λ -chain genes. Two explanations have been proposed to explain this: (a) the ordered rearrangement theory, which postulates that light-chain gene rearrangement in the pre-B cell is first attempted at the κ locus, and that only upon failure to produce a functional κ chain is there an attempt to rearrange the λ locus; and (b) the stochastic theory, which postulates that rearrangement at the λ locus proceeds at a rate that is intrinsically much slower than that at the κ locus. We show here that λ -chain genes are generated whether or not the κ locus has lost its germ-line arrangement, a result that is compatible only with the stochastic theory.},
doi = {10.1155/1990/56014},
journal = {Developmental Immunology},
number = 1,
volume = 1,
place = {Country unknown/Code not available},
year = {Mon Jan 01 00:00:00 EST 1990},
month = {Mon Jan 01 00:00:00 EST 1990}
}
https://doi.org/10.1155/1990/56014