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Title: The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation

Here, we investigate and quantify the effects of pH and salt concentration on the charge regulation of the bacteriophage PP7 capsid. These effects are found to be extremely important and substantial, introducing qualitative changes in the charge state of the capsid such as a transition from net-positive to net-negative charge depending on the solution pH. The overall charge of the virus capsid arises as a consequence of a complicated balance with the chemical dissociation equilibrium of the amino acids and the electrostatic interaction between them, and the translational entropy of the mobile solution ions, i.e., counterion release. We show that to properly describe and predict the charging equilibrium of viral capsids in general, one needs to include molecular details as exemplified by the acid-base equilibrium of the detailed distribution of amino acids in the proteinaceous capsid shell.
Authors:
 [1] ;  [2] ;  [1] ;  [3]
  1. Northwestern Univ., Evanston, IL (United States)
  2. Max Planck Institute for Biology of Ageing, Cologne (Germany)
  3. Jozef Stefan Institute, Ljubljana (Slovenia); Univ. of Ljubljana, Ljubljana (Slovenia); Univ. of Massachusetts, Amherst, MA (United States)
Publication Date:
Grant/Contract Number:
SC0008176
Type:
Published Article
Journal Name:
Biophysical Journal
Additional Journal Information:
Journal Volume: 107; Journal Issue: 8; Journal ID: ISSN 0006-3495
Publisher:
Elsevier
Research Org:
Univ. of Massachusetts, Amherst, MA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1227563
Alternate Identifier(s):
OSTI ID: 1441154

Nap, Rikkert J., Bozic, Anze Losdorfer, Szleifer, Igal, and Podgornik, Rudolf. The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation. United States: N. p., Web. doi:10.1016/j.bpj.2014.08.032.
Nap, Rikkert J., Bozic, Anze Losdorfer, Szleifer, Igal, & Podgornik, Rudolf. The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation. United States. doi:10.1016/j.bpj.2014.08.032.
Nap, Rikkert J., Bozic, Anze Losdorfer, Szleifer, Igal, and Podgornik, Rudolf. 2014. "The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation". United States. doi:10.1016/j.bpj.2014.08.032.
@article{osti_1227563,
title = {The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation},
author = {Nap, Rikkert J. and Bozic, Anze Losdorfer and Szleifer, Igal and Podgornik, Rudolf},
abstractNote = {Here, we investigate and quantify the effects of pH and salt concentration on the charge regulation of the bacteriophage PP7 capsid. These effects are found to be extremely important and substantial, introducing qualitative changes in the charge state of the capsid such as a transition from net-positive to net-negative charge depending on the solution pH. The overall charge of the virus capsid arises as a consequence of a complicated balance with the chemical dissociation equilibrium of the amino acids and the electrostatic interaction between them, and the translational entropy of the mobile solution ions, i.e., counterion release. We show that to properly describe and predict the charging equilibrium of viral capsids in general, one needs to include molecular details as exemplified by the acid-base equilibrium of the detailed distribution of amino acids in the proteinaceous capsid shell.},
doi = {10.1016/j.bpj.2014.08.032},
journal = {Biophysical Journal},
number = 8,
volume = 107,
place = {United States},
year = {2014},
month = {10}
}