The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation
Abstract
Here, we investigate and quantify the effects of pH and salt concentration on the charge regulation of the bacteriophage PP7 capsid. These effects are found to be extremely important and substantial, introducing qualitative changes in the charge state of the capsid such as a transition from net-positive to net-negative charge depending on the solution pH. The overall charge of the virus capsid arises as a consequence of a complicated balance with the chemical dissociation equilibrium of the amino acids and the electrostatic interaction between them, and the translational entropy of the mobile solution ions, i.e., counterion release. We show that to properly describe and predict the charging equilibrium of viral capsids in general, one needs to include molecular details as exemplified by the acid-base equilibrium of the detailed distribution of amino acids in the proteinaceous capsid shell.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Massachusetts, Amherst, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1227563
- Alternate Identifier(s):
- OSTI ID: 1441154
- Grant/Contract Number:
- SC0008176
- Resource Type:
- Published Article
- Journal Name:
- Biophysical Journal
- Additional Journal Information:
- Journal Name: Biophysical Journal Journal Volume: 107 Journal Issue: 8; Journal ID: ISSN 0006-3495
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Nap, Rikkert J., Božič, Anže Lošdorfer, Szleifer, Igal, and Podgornik, Rudolf. The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation. United States: N. p., 2014.
Web. doi:10.1016/j.bpj.2014.08.032.
Nap, Rikkert J., Božič, Anže Lošdorfer, Szleifer, Igal, & Podgornik, Rudolf. The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation. United States. https://doi.org/10.1016/j.bpj.2014.08.032
Nap, Rikkert J., Božič, Anže Lošdorfer, Szleifer, Igal, and Podgornik, Rudolf. Wed .
"The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation". United States. https://doi.org/10.1016/j.bpj.2014.08.032.
@article{osti_1227563,
title = {The Role of Solution Conditions in the Bacteriophage PP7 Capsid Charge Regulation},
author = {Nap, Rikkert J. and Božič, Anže Lošdorfer and Szleifer, Igal and Podgornik, Rudolf},
abstractNote = {Here, we investigate and quantify the effects of pH and salt concentration on the charge regulation of the bacteriophage PP7 capsid. These effects are found to be extremely important and substantial, introducing qualitative changes in the charge state of the capsid such as a transition from net-positive to net-negative charge depending on the solution pH. The overall charge of the virus capsid arises as a consequence of a complicated balance with the chemical dissociation equilibrium of the amino acids and the electrostatic interaction between them, and the translational entropy of the mobile solution ions, i.e., counterion release. We show that to properly describe and predict the charging equilibrium of viral capsids in general, one needs to include molecular details as exemplified by the acid-base equilibrium of the detailed distribution of amino acids in the proteinaceous capsid shell.},
doi = {10.1016/j.bpj.2014.08.032},
journal = {Biophysical Journal},
number = 8,
volume = 107,
place = {United States},
year = {Wed Oct 01 00:00:00 EDT 2014},
month = {Wed Oct 01 00:00:00 EDT 2014}
}
https://doi.org/10.1016/j.bpj.2014.08.032
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