Structural and functional characterization of the hazelnut allergen Cor a 8

Offermann, Lesa R.; Bublin, Merima; Perdue, Makenzie L.; Pfeifer, Sabine; Dubiela, Pawel; Borowski, Tomasz; Chruszcz, Maksymilian; Hoffmann-Sommergruber, Karin

doi:10.1021/acs.jafc.5b03534

Title: Structural and functional characterization of the hazelnut allergen Cor a 8

Journal Article · Mon Sep 28 00:00:00 EDT 2015 · Journal of Agricultural and Food Chemistry

DOI: https://doi.org/10.1021/acs.jafc.5b03534 · OSTI ID:1224206

Offermann, Lesa R. ^[1]; Bublin, Merima ^[2]; Perdue, Makenzie L. ^[3]; Pfeifer, Sabine ^[2]; Dubiela, Pawel ^[2]; Borowski, Tomasz ^[4]; Chruszcz, Maksymilian ^[3]; Hoffmann-Sommergruber, Karin ^[2]

Univ. of South Carolina, Columbia, SC (United States); Davidson College, Davidson, NC (United States)
Univ. of Vienna, Vienna (Austria)
Univ. of South Carolina, Columbia, SC (United States)
Polish Academy of Sciences, Krakow (Poland)

Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious, which may contribute to limited cross-reactivity between peach and hazelnut allergens. The differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-06CH11357; W-31109-ENG-38

OSTI ID:: 1224206

Journal Information:: Journal of Agricultural and Food Chemistry, Journal Name: Journal of Agricultural and Food Chemistry Journal Issue: 41 Vol. 63; ISSN 0021-8561

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
allergen
food allergy
hazelnut
nonspecific lipid transfer protein
protein crystallization

Title: Structural and functional characterization of the hazelnut allergen Cor a 8

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