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Title: Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus

Abstract

Hydrogen gas is a potential renewable alternative energy carrier that could be used in the future to help supplement humanity’s growing energy needs. Unfortunately, current industrial methods for hydrogen production are expensive or environmentally unfriendly. In recent years research has focused on biological mechanisms for hydrogen production and specifically on hydrogenases, the enzyme responsible for catalyzing the reduction of protons to generate hydrogen. In particular, a better understanding of this enzyme might allow us to generate hydrogen that does not use expensive metals, such as platinum, as catalysts. The soluble hydrogenase I (SHI) from the hyperthermophile Pyrococcus furiosus , a member of the euryarchaeota, has been studied extensively and used in various biotechnological applications. This review summarizes the strategies used in engineering and characterizing three different forms of SHI and the properties of the recombinant enzymes. SHI has also been used in in vitro systems for hydrogen production and NADPH generation and these systems are also discussed.

Authors:
 [1];  [1];  [1];  [1]
  1. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1223549
Grant/Contract Number:  
FG05-95ER20175
Resource Type:
Published Article
Journal Name:
Archaea
Additional Journal Information:
Journal Name: Archaea Journal Volume: 2015; Journal ID: ISSN 1472-3646
Publisher:
Hindawi
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wu, Chang-Hao, McTernan, Patrick M., Walter, Mary E., and Adams, Michael W. W. Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus. United States: N. p., 2015. Web. doi:10.1155/2015/912582.
Wu, Chang-Hao, McTernan, Patrick M., Walter, Mary E., & Adams, Michael W. W. Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus. United States. doi:10.1155/2015/912582.
Wu, Chang-Hao, McTernan, Patrick M., Walter, Mary E., and Adams, Michael W. W. Thu . "Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus". United States. doi:10.1155/2015/912582.
@article{osti_1223549,
title = {Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus},
author = {Wu, Chang-Hao and McTernan, Patrick M. and Walter, Mary E. and Adams, Michael W. W.},
abstractNote = {Hydrogen gas is a potential renewable alternative energy carrier that could be used in the future to help supplement humanity’s growing energy needs. Unfortunately, current industrial methods for hydrogen production are expensive or environmentally unfriendly. In recent years research has focused on biological mechanisms for hydrogen production and specifically on hydrogenases, the enzyme responsible for catalyzing the reduction of protons to generate hydrogen. In particular, a better understanding of this enzyme might allow us to generate hydrogen that does not use expensive metals, such as platinum, as catalysts. The soluble hydrogenase I (SHI) from the hyperthermophile Pyrococcus furiosus , a member of the euryarchaeota, has been studied extensively and used in various biotechnological applications. This review summarizes the strategies used in engineering and characterizing three different forms of SHI and the properties of the recombinant enzymes. SHI has also been used in in vitro systems for hydrogen production and NADPH generation and these systems are also discussed.},
doi = {10.1155/2015/912582},
journal = {Archaea},
number = ,
volume = 2015,
place = {United States},
year = {2015},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1155/2015/912582

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Works referenced in this record:

A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003

  • Sapra, R.; Bagramyan, K.; Adams, M. W. W.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 13, p. 7545-7550
  • DOI: 10.1073/pnas.1331436100

Occurrence, Classification, and Biological Function of Hydrogenases:� An Overview
journal, October 2007

  • Vignais, Paulette M.; Billoud, Bernard
  • Chemical Reviews, Vol. 107, Issue 10, p. 4206-4272
  • DOI: 10.1021/cr050196r

Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus : A key role for NADPH
journal, October 1994


Spontaneous High-Yield Production of Hydrogen from Cellulosic Materials and Water Catalyzed by Enzyme Cocktails
journal, February 2009

  • Ye, Xinhao; Wang, Yiran; Hopkins, Robert?C.
  • ChemSusChem, Vol. 2, Issue 2, p. 149-152
  • DOI: 10.1002/cssc.200900017

Maturation of Hydrogenases
book, September 2006


In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996

  • Woodward, Jonathan; Mattingly, Susan M.; Danson, Michael
  • Nature Biotechnology, Vol. 14, Issue 7, p. 872-874
  • DOI: 10.1038/nbt0796-872

Enzymatic production of biohydrogen: Biotechnology
journal, June 2000

  • Woodward, Jonathan; Orr, Mark; Cordray, Kimberley
  • Nature, Vol. 405, Issue 6790, p. 1014-1015
  • DOI: 10.1038/35016633

High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007

  • Zhang, Y.-H. Percival; Evans, Barbara R.; Mielenz, Jonathan R.
  • PLoS ONE, Vol. 2, Issue 5, Article No. e456
  • DOI: 10.1371/journal.pone.0000456

Homologous Expression of a Subcomplex of Pyrococcus furiosus Hydrogenase that Interacts with Pyruvate Ferredoxin Oxidoreductase
journal, October 2011


Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production
journal, May 2010