Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus
Abstract
Hydrogen gas is a potential renewable alternative energy carrier that could be used in the future to help supplement humanity’s growing energy needs. Unfortunately, current industrial methods for hydrogen production are expensive or environmentally unfriendly. In recent years research has focused on biological mechanisms for hydrogen production and specifically on hydrogenases, the enzyme responsible for catalyzing the reduction of protons to generate hydrogen. In particular, a better understanding of this enzyme might allow us to generate hydrogen that does not use expensive metals, such as platinum, as catalysts. The soluble hydrogenase I (SHI) from the hyperthermophile Pyrococcus furiosus , a member of the euryarchaeota, has been studied extensively and used in various biotechnological applications. This review summarizes the strategies used in engineering and characterizing three different forms of SHI and the properties of the recombinant enzymes. SHI has also been used in in vitro systems for hydrogen production and NADPH generation and these systems are also discussed.
- Authors:
-
- Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1223549
- Alternate Identifier(s):
- OSTI ID: 1626228
- Grant/Contract Number:
- FG05-95ER20175
- Resource Type:
- Published Article
- Journal Name:
- Archaea
- Additional Journal Information:
- Journal Name: Archaea Journal Volume: 2015; Journal ID: ISSN 1472-3646
- Publisher:
- Hindawi
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology
Citation Formats
Wu, Chang-Hao, McTernan, Patrick M., Walter, Mary E., and Adams, Michael W. W. Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus. United States: N. p., 2015.
Web. doi:10.1155/2015/912582.
Wu, Chang-Hao, McTernan, Patrick M., Walter, Mary E., & Adams, Michael W. W. Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus. United States. https://doi.org/10.1155/2015/912582
Wu, Chang-Hao, McTernan, Patrick M., Walter, Mary E., and Adams, Michael W. W. Thu .
"Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus". United States. https://doi.org/10.1155/2015/912582.
@article{osti_1223549,
title = {Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus},
author = {Wu, Chang-Hao and McTernan, Patrick M. and Walter, Mary E. and Adams, Michael W. W.},
abstractNote = {Hydrogen gas is a potential renewable alternative energy carrier that could be used in the future to help supplement humanity’s growing energy needs. Unfortunately, current industrial methods for hydrogen production are expensive or environmentally unfriendly. In recent years research has focused on biological mechanisms for hydrogen production and specifically on hydrogenases, the enzyme responsible for catalyzing the reduction of protons to generate hydrogen. In particular, a better understanding of this enzyme might allow us to generate hydrogen that does not use expensive metals, such as platinum, as catalysts. The soluble hydrogenase I (SHI) from the hyperthermophile Pyrococcus furiosus , a member of the euryarchaeota, has been studied extensively and used in various biotechnological applications. This review summarizes the strategies used in engineering and characterizing three different forms of SHI and the properties of the recombinant enzymes. SHI has also been used in in vitro systems for hydrogen production and NADPH generation and these systems are also discussed.},
doi = {10.1155/2015/912582},
journal = {Archaea},
number = ,
volume = 2015,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1155/2015/912582
https://doi.org/10.1155/2015/912582
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 10 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003
- Sapra, R.; Bagramyan, K.; Adams, M. W. W.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 13, p. 7545-7550
Cleaning the Air and Improving Health with Hydrogen Fuel-Cell Vehicles
journal, June 2005
- Jacobson, M. Z.
- Science, Vol. 308, Issue 5730
High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form
journal, March 2015
- Chandrayan, Sanjeev K.; Wu, Chang-Hao; McTernan, Patrick M.
- Protein Expression and Purification, Vol. 107
Design and characterization of redox enzyme electrodes: new perspectives on established techniques with application to an extremeophilic hydrogenase
journal, March 2005
- Johnston, Wayne; Cooney, Michael J.; Liaw, Bor Yann
- Enzyme and Microbial Technology, Vol. 36, Issue 4
Nickel L-Edge Soft X-ray Spectroscopy of Nickel−Iron Hydrogenases and Model CompoundsEvidence for High-Spin Nickel(II) in the Active Enzyme
journal, November 2000
- Wang, Hongxin; Ralston, C. Y.; Patil, D. S.
- Journal of the American Chemical Society, Vol. 122, Issue 43
Spontaneous High-Yield Production of Hydrogen from Cellulosic Materials and Water Catalyzed by Enzyme Cocktails
journal, February 2009
- Ye, Xinhao; Wang, Yiran; Hopkins, Robert C.
- ChemSusChem, Vol. 2, Issue 2, p. 149-152
In vitro hydrogen photoproduction using Pyrococcus furiosus sulfhydrogenase and TiO2
journal, August 1999
- Selvaggi, A.; Tosi, C.; Barberini, U.
- Journal of Photochemistry and Photobiology A: Chemistry, Vol. 125, Issue 1-3
High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling
journal, April 2015
- Rollin, Joseph A.; Martin del Campo, Julia; Myung, Suwan
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 16
Biological hydrogen production: prospects and challenges
journal, May 2010
- Lee, Hyung-Sool; Vermaas, Wim F. J.; Rittmann, Bruce E.
- Trends in Biotechnology, Vol. 28, Issue 5
Utilization of adsorption effects for the continuous reduction of NADP + with molecular hydrogen by Pyrococcus furiosus hydrogenase
journal, January 2003
- Greiner, L.; Schröder, I.; Müller, D. H.
- Green Chem., Vol. 5, Issue 6
Enzymatic hydrogen production by light-sensitized anodized tubular TiO2 photoanode
journal, April 2008
- Bae, Sanghyun; Shim, Eunjung; Yoon, Jaekyung
- Solar Energy Materials and Solar Cells, Vol. 92, Issue 4
Nickel L-Edge X-ray Absorption Spectroscopy of Pyrococcus furiosus Hydrogenase
journal, May 1995
- van Elp, J.; Peng, G.; Zhou, Z. H.
- Inorganic Chemistry, Vol. 34, Issue 10
The hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus: From basic research to possible future applications
journal, October 1996
- Pedroni, P.
- International Journal of Hydrogen Energy, Vol. 21, Issue 10
In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996
- Woodward, Jonathan; Mattingly, Susan M.; Danson, Michael
- Nature Biotechnology, Vol. 14, Issue 7, p. 872-874
Enzymatic production of biohydrogen: Biotechnology
journal, June 2000
- Woodward, Jonathan; Orr, Mark; Cordray, Kimberley
- Nature, Vol. 405, Issue 6790, p. 1014-1015
On the relationship between affinity for molecular hydrogen and the physiological directionality of hydrogenases
journal, February 2005
- van Haaster, D. J.; Hagedoorn, P. -L.; Jongejan, J. A.
- Biochemical Society Transactions, Vol. 33, Issue 1
Homologous Expression of a Subcomplex of Pyrococcus furiosus Hydrogenase that Interacts with Pyruvate Ferredoxin Oxidoreductase
journal, October 2011
- Hopkins, R. Christopher; Sun, Junsong; Jenney, Francis E.
- PLoS ONE, Vol. 6, Issue 10, Article No.e26569
Redox properties of the sulfhydrogenase from Pyrococcus furiosus
journal, July 1995
- Arendsen, Alexander F.; Veenhuizen, Peter Th. M.; Hagen, Wilfred R.
- FEBS Letters, Vol. 368, Issue 1
Engineering the respiratory membrane-bound hydrogenase of the hyperthermophilic archaeon Pyrococcus furiosus and characterization of the catalytically active cytoplasmic subcomplex
journal, December 2014
- McTernan, P. M.; Chandrayan, S. K.; Wu, C. -H.
- Protein Engineering Design and Selection, Vol. 28, Issue 1
Hydrogen: the wonder fuel
journal, June 1992
- Vezirolu, T.; Barbir, F.
- International Journal of Hydrogen Energy, Vol. 17, Issue 6
Pyrococcus furiosus-immobilized anodized tubular titania cathode in a hydrogen production system
journal, April 2009
- Yoon, Jaekyung; Bae, Sanghyun; Shim, Eunjung
- Journal of Power Sources, Vol. 189, Issue 2
A sweet out-of-the-box solution to the hydrogen economy: is the sugar-powered car science fiction?
journal, January 2009
- Zhang, Y. -H. Percival
- Energy & Environmental Science, Vol. 2, Issue 3
Enzymatic Conversion of Sucrose to Hydrogen
journal, December 1998
- Woodward, J.; Orr, M.
- Biotechnology Progress, Vol. 14, Issue 6
Occurrence, Classification, and Biological Function of Hydrogenases: An Overview
journal, October 2007
- Vignais, Paulette M.; Billoud, Bernard
- Chemical Reviews, Vol. 107, Issue 10, p. 4206-4272
Thermotolerant Hydrogenases: Biological Diversity, Properties, and Biotechnological Applications
journal, January 2008
- Eberly, Jed O.; Ely, Roger L.
- Critical Reviews in Microbiology, Vol. 34, Issue 3-4
Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus : A key role for NADPH
journal, October 1994
- Ma, Kesen; Hao, Zhi; Adams, Michael W.W.
- FEMS Microbiology Letters, Vol. 122, Issue 3, p. 245-250
Maturation of Hydrogenases
book, September 2006
- Böck, August; King, Paul W.; Blokesch, Melanie
- Advances in Microbial Physiology, Vol. 51, p. 1-71
In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose
journal, July 2014
- Myung, Suwan; Rollin, Joseph; You, Chun
- Metabolic Engineering, Vol. 24
On the prosthetic groups of the NiFe sulfhydrogenase from Pyrococcus furiosus: topology, structure, and temperature-dependent redox chemistry
journal, July 1999
- Silva, Pedro J.; de Castro, Baltazar; Hagen, W. R.
- JBIC Journal of Biological Inorganic Chemistry, Vol. 4, Issue 3
Proton-Coupled Electron Transfer Dynamics in the Catalytic Mechanism of a [NiFe]-Hydrogenase
journal, March 2015
- Greene, Brandon L.; Wu, Chang-Hao; McTernan, Patrick M.
- Journal of the American Chemical Society, Vol. 137, Issue 13
Novel Hydrogen Bioreactor and Detection Apparatus
book, January 2014
- Rollin, Joseph A.; Ye, Xinhao; del Campo, Julia Martin
- Bioreactor Engineering Research and Industrial Applications II
Enzymatic Hydrogen Production: Conversion of Renewable Resources for Energy Production
journal, January 2000
- Woodward, Jonathan; Cordray, Kimberley A.; Blanco-Rivera, Maria
- Energy & Fuels, Vol. 14, Issue 1
Refueling hydrogen fuel cell vehicles with 68 proposed refueling stations in California: Measuring deviations from daily travel patterns
journal, February 2014
- Kang, Jee Eun; Brown, Tim; Recker, Will W.
- International Journal of Hydrogen Energy, Vol. 39, Issue 7
Characterization of Hydrogenase from the Hyperthermophilic Archaebacterium, Pyrococcus furiosus
journal, March 1989
- Bryant, F. O.; Adams, M. W.
- Journal of Biological Chemistry, Vol. 264, Issue 9
High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007
- Zhang, Y.-H. Percival; Evans, Barbara R.; Mielenz, Jonathan R.
- PLoS ONE, Vol. 2, Issue 5, Article No. e456
Characterization and in vitro interaction study of a [NiFe] hydrogenase large subunit from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1
journal, January 2012
- Sasaki, Daisuke; Watanabe, Satoshi; Kanai, Tamotsu
- Biochemical and Biophysical Research Communications, Vol. 417, Issue 1
Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production
journal, May 2010
- Sun, Junsong; Hopkins, Robert C.; Jenney, Francis E.
- PLoS ONE, Vol. 5, Issue 5, Article No. e10526
Hydrogenases
journal, March 2014
- Lubitz, Wolfgang; Ogata, Hideaki; Rüdiger, Olaf
- Chemical Reviews, Vol. 114, Issue 8
Mechanism of oxygen detoxification by the surprisingly oxygen-tolerant hyperthermophilic archaeon, Pyrococcus furiosus
journal, October 2012
- Thorgersen, M. P.; Stirrett, K.; Scott, R. A.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 45
High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System
journal, March 2013
- Martín del Campo, Julia S.; Rollin, Joseph; Myung, Suwan
- Angewandte Chemie International Edition, Vol. 52, Issue 17
Enzymes of hydrogen metabolism in Pyrococcus furiosus: P. furiosus enzymes of hydrogen metabolism
journal, November 2000
- Silva, Pedro J.; van den Ban, Eyke C. D.; Wassink, Hans
- European Journal of Biochemistry, Vol. 267, Issue 22
Direct Bioelectrocatalysis by NADP-Reducing Hydrogenase fromPyrococcus furiosus
journal, November 2007
- Voronin, Oleg G.; van Haaster, Daan J.; Karyakina, Elena E.
- Electroanalysis, Vol. 19, Issue 21