Crystal Structures of Escherichia coli Branching Enzyme in Complex with Linear Oligosaccharides
Abstract
Branching enzyme is responsible for all branching of glycogen and starch. It is an unusual member of the α-amylase family because it has both α-1,4-amylase activity and α-1,6-transferase activity. It also does not react with shorter glucans, though it will bind much longer substrates and substrate mimics. In an effort to better understand how branching enzyme interacts with its polymeric substrate, we have determined the structure of Δ112 Escherichia coli branching enzyme bound to maltoheptaose and maltohexaose. Together, these structures define six distinct oligosaccharide binding sites on the surface of E. coli branching enzyme. Most of these binding sites surround the edge of the β-barrel domain and are quite far from the active site. Surprisingly, there is no evidence of oligosaccharide binding in the active site of the enzyme. The closest bound oligosaccharide resides almost 18 Å from the active site. Mutations to conserved residues in binding sites I and VI had a debilitating effect on the activity of the enzyme.
- Authors:
-
- Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, United States
- Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, United States, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, United States
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
- OSTI Identifier:
- 1223543
- Alternate Identifier(s):
- OSTI ID: 1498300
- Grant/Contract Number:
- FG02-06ER15822
- Resource Type:
- Published Article
- Journal Name:
- Biochemistry
- Additional Journal Information:
- Journal Name: Biochemistry Journal Volume: 54 Journal Issue: 40; Journal ID: ISSN 0006-2960
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Peptides and proteins; Carbohydrates; Oligosaccharides; Molecules; Screening assays
Citation Formats
Feng, Lei, Fawaz, Remie, Hovde, Stacy, Gilbert, Lindsey, Chiou, Janice, and Geiger, James H. Crystal Structures of Escherichia coli Branching Enzyme in Complex with Linear Oligosaccharides. United States: N. p., 2015.
Web. doi:10.1021/acs.biochem.5b00228.
Feng, Lei, Fawaz, Remie, Hovde, Stacy, Gilbert, Lindsey, Chiou, Janice, & Geiger, James H. Crystal Structures of Escherichia coli Branching Enzyme in Complex with Linear Oligosaccharides. United States. https://doi.org/10.1021/acs.biochem.5b00228
Feng, Lei, Fawaz, Remie, Hovde, Stacy, Gilbert, Lindsey, Chiou, Janice, and Geiger, James H. Mon .
"Crystal Structures of Escherichia coli Branching Enzyme in Complex with Linear Oligosaccharides". United States. https://doi.org/10.1021/acs.biochem.5b00228.
@article{osti_1223543,
title = {Crystal Structures of Escherichia coli Branching Enzyme in Complex with Linear Oligosaccharides},
author = {Feng, Lei and Fawaz, Remie and Hovde, Stacy and Gilbert, Lindsey and Chiou, Janice and Geiger, James H.},
abstractNote = {Branching enzyme is responsible for all branching of glycogen and starch. It is an unusual member of the α-amylase family because it has both α-1,4-amylase activity and α-1,6-transferase activity. It also does not react with shorter glucans, though it will bind much longer substrates and substrate mimics. In an effort to better understand how branching enzyme interacts with its polymeric substrate, we have determined the structure of Δ112 Escherichia coli branching enzyme bound to maltoheptaose and maltohexaose. Together, these structures define six distinct oligosaccharide binding sites on the surface of E. coli branching enzyme. Most of these binding sites surround the edge of the β-barrel domain and are quite far from the active site. Surprisingly, there is no evidence of oligosaccharide binding in the active site of the enzyme. The closest bound oligosaccharide resides almost 18 Å from the active site. Mutations to conserved residues in binding sites I and VI had a debilitating effect on the activity of the enzyme.},
doi = {10.1021/acs.biochem.5b00228},
journal = {Biochemistry},
number = 40,
volume = 54,
place = {United States},
year = {Mon Aug 17 00:00:00 EDT 2015},
month = {Mon Aug 17 00:00:00 EDT 2015}
}
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