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Title: High-resolution genetic mapping of allelic variants associated with cell wall chemistry in Populus

We report the identification of six genetic loci and the allelic-variants associated with Populus cell wall phenotypes determined independently using pyrolysis Molecular Beam Mass Spectrometry (pyMBMS), saccharification assay and wet chemistry in two partially overlapping populations of P. trichocarpa genotypes sampled from multiple environments in the Pacific Northwest of North America. All 6 variants co-located with a quantitative trait locus (QTL) hotspot on chromosome XIV for lignin content, syringyl to guaiacyl (S/G) ratio, 5- and 6- carbon sugars identified in an interspecific P. trichocarpa x P. deltoides pseudo-backcross mapping pedigree. Genomic intervals containing an amino acid transporter, a MYB transcription factor, an angustifolia CtBP transcription factor, a copper transport protein ATOX1-related, a Ca 2+ transporting ATPase and a protein kinase were identified within 5 QTL regions. Each interval contained single nucleotide polymorphisms (SNPs) that were significantly associated to cell-wall phenotypes, with associations exceeding the chromosome-wise Bonferroni-adjusted p-values in at least one environment. cDNA sequencing for allelic variants of 3 of the 6 genes identified polymorphisms leading to premature stop codons in the MYB transcription factor and protein kinase. On the other hand, variants of the Angustifolia CtBP transcription factor exhibited a polyglutamine (PolyQ) length polymorphism. Results from transient protoplast assaysmore » suggested that each of the polymorphisms conferred allelic differences in activation of cellulose, hemicelluloses and lignin pathway marker genes, with truncated and short PolyQ alleles exhibiting significantly reduced marker gene activation. Genes identified in this study represent novel targets for reducing cell wall recalcitrance for lignocellulosic biofuels production using plant biomass.« less
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  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. West Virginia Univ., Morgantown, WV (United States)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  4. Czech Univ. of Life Sciences, Prague (Czech Republic); Univ. of British Columbia, Vancouver (Canada)
  5. Univ. of British Columbia, Vancouver, BC (Canada)
  6. Univ. of British Columbia, Vancouver, BC (Canada)er
  7. U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA (United States)
Publication Date:
Report Number(s):
Journal ID: ISSN 1471-2164; KP1601050; ERKP695
Grant/Contract Number:
AC05-00OR22725; AC05-00OR2272; AC02-05CH11231
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 16; Journal Issue: 24; Journal ID: ISSN 1471-2164
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; QTL cloning; association genetics; cell wall recalcitrance; lignin; cellulose; hemicellulose; 09 BIOMASS FUELS
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1220715