Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling

Journal Article · Mon Dec 31 23:00:00 EST 2007 · Spectroscopy

DOI: https://doi.org/10.1155/2008/715905 · OSTI ID:1198015

O'Maille, Grace ^[1]; Go, Eden P. ^[1]; Hoang, Linh ^[1]; Want, Elizabeth J. ^[1]; Smith, Colin ^[1]; O'Maille, Paul ^[2]; NordstrÖm, Anders ^[1]; Morita, Hirotoshi ^[1]; Qin, Chuan ^[1]; Uritboonthai, Wilasinee ^[1]; Apon, Junefredo ^[1]; Moore, Richard ^[3]; Garrett, James ^[3]; Siuzdak, Gary ^[1]

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1. The Center for Mass Spectrometry and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA-92037, USA
2. Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA-92037, USA
3. Becton Dickinson, Sparks, MD-21152, USA

Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.

• Sponsoring Organization: USDOE
• OSTI ID: 1198015
• Journal Information: Spectroscopy, Journal Name: Spectroscopy Journal Issue: 5 Vol. 22; ISSN 0712-4813
• Publisher: Hindawi Publishing CorporationCopyright Statement
• Country of Publication: Country unknown/Code not available
• Language: English