Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells
Abstract
One central question is how specificity in cellular responses to the eukaryotic second messenger Ca2+ is achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system for in depth investigation of Ca2+-signaling specificity in plants. In intact guard cells, abscisic acid (ABA) enhances (primes) the Ca2+-sensitivity of downstream signaling events that result in activation of S-type anion channels during stomatal closure, providing a specificity mechanism in Ca2+-signaling. However, the underlying genetic and biochemical mechanisms remain unknown. Here we show impairment of ABA signal transduction in stomata of calcium-dependent protein kinase quadruple mutant plants. Interestingly, protein phosphatase 2Cs prevent non-specific Ca2+-signaling. Moreover, we demonstrate an unexpected interdependence of the Ca2+-dependent and Ca2+-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at two key phosphorylation sites in SLAC1. We identify novel mechanisms ensuring specificity and robustness within stomatal Ca2+-signaling on a cellular, genetic, and biochemical level.
- Authors:
-
- Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, United States
- Publication Date:
- Research Org.:
- Univ. of California, San Diego, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); National Science Foundation (NSF)
- OSTI Identifier:
- 1196443
- Alternate Identifier(s):
- OSTI ID: 1198705; OSTI ID: 1411725
- Grant/Contract Number:
- FG02-03ER15449; GM060396-ES010337; MCB1414339
- Resource Type:
- Published Article
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Name: eLife Journal Volume: 4; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Brandt, Benjamin, Munemasa, Shintaro, Wang, Cun, Nguyen, Desiree, Yong, Taiming, Yang, Paul G., Poretsky, Elly, Belknap, Thomas F., Waadt, Rainer, Alemán, Fernando, and Schroeder, Julian I. Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells. United States: N. p., 2015.
Web. doi:10.7554/eLife.03599.
Brandt, Benjamin, Munemasa, Shintaro, Wang, Cun, Nguyen, Desiree, Yong, Taiming, Yang, Paul G., Poretsky, Elly, Belknap, Thomas F., Waadt, Rainer, Alemán, Fernando, & Schroeder, Julian I. Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells. United States. https://doi.org/10.7554/eLife.03599
Brandt, Benjamin, Munemasa, Shintaro, Wang, Cun, Nguyen, Desiree, Yong, Taiming, Yang, Paul G., Poretsky, Elly, Belknap, Thomas F., Waadt, Rainer, Alemán, Fernando, and Schroeder, Julian I. Mon .
"Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells". United States. https://doi.org/10.7554/eLife.03599.
@article{osti_1196443,
title = {Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells},
author = {Brandt, Benjamin and Munemasa, Shintaro and Wang, Cun and Nguyen, Desiree and Yong, Taiming and Yang, Paul G. and Poretsky, Elly and Belknap, Thomas F. and Waadt, Rainer and Alemán, Fernando and Schroeder, Julian I.},
abstractNote = {One central question is how specificity in cellular responses to the eukaryotic second messenger Ca2+ is achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system for in depth investigation of Ca2+-signaling specificity in plants. In intact guard cells, abscisic acid (ABA) enhances (primes) the Ca2+-sensitivity of downstream signaling events that result in activation of S-type anion channels during stomatal closure, providing a specificity mechanism in Ca2+-signaling. However, the underlying genetic and biochemical mechanisms remain unknown. Here we show impairment of ABA signal transduction in stomata of calcium-dependent protein kinase quadruple mutant plants. Interestingly, protein phosphatase 2Cs prevent non-specific Ca2+-signaling. Moreover, we demonstrate an unexpected interdependence of the Ca2+-dependent and Ca2+-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at two key phosphorylation sites in SLAC1. We identify novel mechanisms ensuring specificity and robustness within stomatal Ca2+-signaling on a cellular, genetic, and biochemical level.},
doi = {10.7554/eLife.03599},
journal = {eLife},
number = ,
volume = 4,
place = {United States},
year = {Mon Jul 20 00:00:00 EDT 2015},
month = {Mon Jul 20 00:00:00 EDT 2015}
}
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