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Title: Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria

Abstract

Physical protection of soil carbon (C) is one of the important components of C storage. However, its exact mechanisms are still not sufficiently lucid. The goal of this study was to explore the influence of soil structure, that is, soil pore spatial arrangements, with and without presence of plant residue on (i) decomposition of added plant residue, (ii) CO₂ emission from soil, and (iii) structure of soil bacterial communities. The study consisted of several soil incubation experiments with samples of contrasting pore characteristics with/without plant residue, accompanied by X-ray micro-tomographic analyses of soil pores and by microbial community analysis of amplified 16S–18S rRNA genes via pyrosequencing. We observed that in the samples with substantial presence of air-filled well-connected large (>30 µm) pores, 75–80% of the added plant residue was decomposed, cumulative CO₂ emission constituted 1,200 µm C g⁻¹ soil, and movement of C from decomposing plant residue into adjacent soil was insignificant. In the samples with greater abundance of water-filled small pores, 60% of the added plant residue was decomposed, cumulative CO₂ emission constituted 2,000 µm C g⁻¹ soil, and the movement of residue C into adjacent soil was substantial. In the absence of plant residue the influence of poremore » characteristics on CO₂ emission, that is on decomposition of the native soil organic C, was negligible. The microbial communities on the plant residue in the samples with large pores had more microbial groups known to be cellulose decomposers, that is, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes, while a number of oligotrophic Acidobacteria groups were more abundant on the plant residue from the samples with small pores. This study provides the first experimental evidence that characteristics of soil pores and their air/water flow status determine the phylogenetic composition of the local microbial community and directions and magnitudes of soil C decomposition processes.« less

Authors:
 [1];  [2];  [2];  [2];  [3];  [4]
  1. Institute for Advanced Sustainability Studies, Potsdam (Germany). IASS-Global Soil Forum.
  2. Michigan State Univ., East Lansing, MI (United States). Dept. of Plant, Soil and Microbial Sciences.
  3. Michigan State Univ., East Lansing, MI (United States). Dept. of Microbiology and Molecular Genetics.
  4. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGN
OSTI Identifier:
1188810
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 10; Journal Issue: 4; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
ENGLISH
Subject:
54 ENVIRONMENTAL SCIENCES; leaves; carbon dioxide; maize; plant communities; porosity; community structure; fluid dynamics; Shannon index

Citation Formats

Negassa, Wakene C., Guber, Andrey K., Kravchenko, Alexandra N., Marsh, Terence L., Hildebrandt, Britton, and Rivers, Mark L. Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria. United States: N. p., 2015. Web. doi:10.1371/journal.pone.0123999.
Negassa, Wakene C., Guber, Andrey K., Kravchenko, Alexandra N., Marsh, Terence L., Hildebrandt, Britton, & Rivers, Mark L. Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria. United States. doi:10.1371/journal.pone.0123999.
Negassa, Wakene C., Guber, Andrey K., Kravchenko, Alexandra N., Marsh, Terence L., Hildebrandt, Britton, and Rivers, Mark L. Wed . "Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria". United States. doi:10.1371/journal.pone.0123999. https://www.osti.gov/servlets/purl/1188810.
@article{osti_1188810,
title = {Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria},
author = {Negassa, Wakene C. and Guber, Andrey K. and Kravchenko, Alexandra N. and Marsh, Terence L. and Hildebrandt, Britton and Rivers, Mark L.},
abstractNote = {Physical protection of soil carbon (C) is one of the important components of C storage. However, its exact mechanisms are still not sufficiently lucid. The goal of this study was to explore the influence of soil structure, that is, soil pore spatial arrangements, with and without presence of plant residue on (i) decomposition of added plant residue, (ii) CO₂ emission from soil, and (iii) structure of soil bacterial communities. The study consisted of several soil incubation experiments with samples of contrasting pore characteristics with/without plant residue, accompanied by X-ray micro-tomographic analyses of soil pores and by microbial community analysis of amplified 16S–18S rRNA genes via pyrosequencing. We observed that in the samples with substantial presence of air-filled well-connected large (>30 µm) pores, 75–80% of the added plant residue was decomposed, cumulative CO₂ emission constituted 1,200 µm C g⁻¹ soil, and movement of C from decomposing plant residue into adjacent soil was insignificant. In the samples with greater abundance of water-filled small pores, 60% of the added plant residue was decomposed, cumulative CO₂ emission constituted 2,000 µm C g⁻¹ soil, and the movement of residue C into adjacent soil was substantial. In the absence of plant residue the influence of pore characteristics on CO₂ emission, that is on decomposition of the native soil organic C, was negligible. The microbial communities on the plant residue in the samples with large pores had more microbial groups known to be cellulose decomposers, that is, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes, while a number of oligotrophic Acidobacteria groups were more abundant on the plant residue from the samples with small pores. This study provides the first experimental evidence that characteristics of soil pores and their air/water flow status determine the phylogenetic composition of the local microbial community and directions and magnitudes of soil C decomposition processes.},
doi = {10.1371/journal.pone.0123999},
journal = {PLoS ONE},
number = 4,
volume = 10,
place = {United States},
year = {2015},
month = {7}
}

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