Control of mechanically activated polymersome fusion: Factors affecting fusion

Henderson, Ian M.; Paxton, Walter F.

doi:10.1002/polb.23650

Title: Control of mechanically activated polymersome fusion: Factors affecting fusion

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Abstract

ABSTRACT Previously, it was found that extruded (200 nm) polymer vesicles are capable of fusion into giant polymersomes using agitation in the presence of salt. In this study, several factors contributing to this phenomenon, including the effects of (i) polymer vesicle concentration, (ii) agitation speed and duration, and (iii) variation of the salt and its concentration are investigated. To accomplish these goals dynamic light scattering is used in conjunction with fluorescence microscopy, which provides insight into vesicles above the practical limit for DLS characterization. Increasing the concentration of the polymer dramatically increases the production of giant vesicles through the increased collisions of polymersomes. Likewise, increasing the frequency of agitation increases the efficiency of fusion, although ultimately the size of vesicle that could be produced is limited due to the high shear involved. Finally, salt‐mediation of the fusion process was not limited to NaCl, but is instead a general effect facilitated by the presence of solvated ionic compounds, albeit with different salts initiating fusion at different concentrations. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2015 , 53 , 297–303

Authors:

Henderson, Ian M. ^[1]; Paxton, Walter F. ^[1]

Sandia National Laboratories (SNL), Albuquerque NM (United States). Center for Integrated Nanotechnologies

Publication Date:: Mon Dec 15 00:00:00 EST 2014

Research Org.:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org.:: USDOE National Nuclear Security Administration (NNSA)

OSTI Identifier:: 1182985

Alternate Identifier(s):: OSTI ID: 1401363

Report Number(s):: SAND-2014-15541J
Journal ID: ISSN 0887-6266; 533715

Grant/Contract Number:: AC04-94AL85000

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Polymer Science. Part B, Polymer Physics

Additional Journal Information:: Journal Volume: 53; Journal Issue: 4; Journal ID: ISSN 0887-6266

Publisher:: Wiley

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE

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                    Henderson, Ian M., and Paxton, Walter F. Control of mechanically activated polymersome fusion: Factors affecting fusion.  United States: N. p., 2014. 
Web.  doi:10.1002/polb.23650.

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                    Henderson, Ian M., & Paxton, Walter F. Control of mechanically activated polymersome fusion: Factors affecting fusion.  United States.  https://doi.org/10.1002/polb.23650

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                    Henderson, Ian M., and Paxton, Walter F. Mon .  
"Control of mechanically activated polymersome fusion: Factors affecting fusion".  United States.  https://doi.org/10.1002/polb.23650.  https://www.osti.gov/servlets/purl/1182985.

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@article{osti_1182985,

  title        = {Control of mechanically activated polymersome fusion: Factors affecting fusion},

  author       = {Henderson, Ian M. and Paxton, Walter F.},

  abstractNote = {ABSTRACT Previously, it was found that extruded (200 nm) polymer vesicles are capable of fusion into giant polymersomes using agitation in the presence of salt. In this study, several factors contributing to this phenomenon, including the effects of (i) polymer vesicle concentration, (ii) agitation speed and duration, and (iii) variation of the salt and its concentration are investigated. To accomplish these goals dynamic light scattering is used in conjunction with fluorescence microscopy, which provides insight into vesicles above the practical limit for DLS characterization. Increasing the concentration of the polymer dramatically increases the production of giant vesicles through the increased collisions of polymersomes. Likewise, increasing the frequency of agitation increases the efficiency of fusion, although ultimately the size of vesicle that could be produced is limited due to the high shear involved. Finally, salt‐mediation of the fusion process was not limited to NaCl, but is instead a general effect facilitated by the presence of solvated ionic compounds, albeit with different salts initiating fusion at different concentrations. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2015 , 53 , 297–303},

  doi          = {10.1002/polb.23650},

  journal      = {Journal of Polymer Science. Part B, Polymer Physics},

  number       = 4,

  volume       = 53,

  place        = {United States},

  year         = {Mon Dec 15 00:00:00 EST 2014},

  month        = {Mon Dec 15 00:00:00 EST 2014}

}

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