Atomic structure of the Y complex of the nuclear pore
Abstract
The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. In this paper, we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Finally, evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.
- Authors:
-
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Biology
- Publication Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health (NIH) (United States); National Science Foundation (NSF)
- OSTI Identifier:
- 1182318
- Grant/Contract Number:
- AC02-06CH11357; GM103403; R01GM77537; T32GM007287; 1122374
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Structural & Molecular Biology
- Additional Journal Information:
- Journal Volume: 22; Journal Issue: 5; Journal ID: ISSN 1545-9993
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; X-ray crystallography
Citation Formats
Kelley, Kotaro, Knockenhauer, Kevin E., Kabachinski, Greg, and Schwartz, Thomas U. Atomic structure of the Y complex of the nuclear pore. United States: N. p., 2015.
Web. doi:10.1038/nsmb.2998.
Kelley, Kotaro, Knockenhauer, Kevin E., Kabachinski, Greg, & Schwartz, Thomas U. Atomic structure of the Y complex of the nuclear pore. United States. https://doi.org/10.1038/nsmb.2998
Kelley, Kotaro, Knockenhauer, Kevin E., Kabachinski, Greg, and Schwartz, Thomas U. Mon .
"Atomic structure of the Y complex of the nuclear pore". United States. https://doi.org/10.1038/nsmb.2998. https://www.osti.gov/servlets/purl/1182318.
@article{osti_1182318,
title = {Atomic structure of the Y complex of the nuclear pore},
author = {Kelley, Kotaro and Knockenhauer, Kevin E. and Kabachinski, Greg and Schwartz, Thomas U.},
abstractNote = {The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. In this paper, we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Finally, evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.},
doi = {10.1038/nsmb.2998},
journal = {Nature Structural & Molecular Biology},
number = 5,
volume = 22,
place = {United States},
year = {Mon Mar 30 00:00:00 EDT 2015},
month = {Mon Mar 30 00:00:00 EDT 2015}
}
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Toward Automatic Reconstruction of a Highly Resolved Tree of Life
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Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats
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Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation
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Quantifying the heterogeneity of macromolecular machines by mass photometry
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Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically‐Encoded Probes for DNA‐PAINT
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Structure Determination of the Nuclear Pore Complex with Three-Dimensional Cryo electron Microscopy
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A short linear motif in scaffold Nup145C connects Y-complex with pre-assembled outer ring Nup82 complex
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Dissecting Torsin/cofactor function at the nuclear envelope: a genetic study
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Relocalization of DNA lesions to the nuclear pore complex
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Molecular determinants of large cargo transport into the nucleus
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Interactome Mapping Reveals the Evolutionary History of the Nuclear Pore Complex
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Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation
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