skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Atomic structure of the Y complex of the nuclear pore

Abstract

The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. In this paper, we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Finally, evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.

Authors:
 [1];  [1];  [1];  [1]
  1. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Biology
Publication Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health (NIH) (United States); National Science Foundation (NSF)
OSTI Identifier:
1182318
Grant/Contract Number:  
AC02-06CH11357; GM103403; R01GM77537; T32GM007287; 1122374
Resource Type:
Accepted Manuscript
Journal Name:
Nature Structural & Molecular Biology
Additional Journal Information:
Journal Volume: 22; Journal Issue: 5; Journal ID: ISSN 1545-9993
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; X-ray crystallography

Citation Formats

Kelley, Kotaro, Knockenhauer, Kevin E., Kabachinski, Greg, and Schwartz, Thomas U. Atomic structure of the Y complex of the nuclear pore. United States: N. p., 2015. Web. doi:10.1038/nsmb.2998.
Kelley, Kotaro, Knockenhauer, Kevin E., Kabachinski, Greg, & Schwartz, Thomas U. Atomic structure of the Y complex of the nuclear pore. United States. doi:10.1038/nsmb.2998.
Kelley, Kotaro, Knockenhauer, Kevin E., Kabachinski, Greg, and Schwartz, Thomas U. Mon . "Atomic structure of the Y complex of the nuclear pore". United States. doi:10.1038/nsmb.2998. https://www.osti.gov/servlets/purl/1182318.
@article{osti_1182318,
title = {Atomic structure of the Y complex of the nuclear pore},
author = {Kelley, Kotaro and Knockenhauer, Kevin E. and Kabachinski, Greg and Schwartz, Thomas U.},
abstractNote = {The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. In this paper, we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Finally, evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.},
doi = {10.1038/nsmb.2998},
journal = {Nature Structural & Molecular Biology},
number = 5,
volume = 22,
place = {United States},
year = {2015},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 24 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Cell type‐specific nuclear pores: a case in point for context‐dependent stoichiometry of molecular machines
journal, January 2013

  • Ori, Alessandro; Banterle, Niccolò; Iskar, Murat
  • Molecular Systems Biology, Vol. 9, Issue 1
  • DOI: 10.1038/msb.2013.4

Overview of the CCP 4 suite and current developments
journal, March 2011

  • Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S0907444910045749

phenix.mr_rosetta: molecular replacement and model rebuilding with Phenix and Rosetta
journal, March 2012

  • Terwilliger, Thomas C.; DiMaio, Frank; Read, Randy J.
  • Journal of Structural and Functional Genomics, Vol. 13, Issue 2
  • DOI: 10.1007/s10969-012-9129-3

Molecular basis for Nup37 and ELY5/ELYS recruitment to the nuclear pore complex
journal, September 2012

  • Bilokapic, S.; Schwartz, T. U.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 38
  • DOI: 10.1073/pnas.1205151109

The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly
journal, April 2003


Molecular architecture of the Nup84–Nup145C–Sec13 edge element in the nuclear pore complex lattice
journal, October 2009

  • Brohawn, Stephen G.; Schwartz, Thomas U.
  • Nature Structural & Molecular Biology, Vol. 16, Issue 11
  • DOI: 10.1038/nsmb.1713

Structural evolution of the membrane-coating module of the nuclear pore complex
journal, September 2012

  • Liu, X.; Mitchell, J. M.; Wozniak, R. W.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 41
  • DOI: 10.1073/pnas.1214557109

Collaboration gets the most out of software
journal, September 2013


Removal of a Single Pore Subcomplex Results in Vertebrate Nuclei Devoid of Nuclear Pores
journal, April 2003


Molecular Architecture and Function of the SEA Complex, a Modulator of the TORC1 Pathway
journal, July 2014

  • Algret, Romain; Fernandez-Martinez, Javier; Shi, Yi
  • Molecular & Cellular Proteomics, Vol. 13, Issue 11
  • DOI: 10.1074/mcp.M114.039388

Toward Automatic Reconstruction of a Highly Resolved Tree of Life
journal, March 2006


Structural and Functional Studies of Nup107/Nup133 Interaction and Its Implications for the Architecture of the Nuclear Pore Complex
journal, June 2008


The Nuclear Pore Complex Has Entered the Atomic Age
journal, September 2009


Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging
journal, July 2013


The Structure of the Scaffold Nucleoporin Nup120 Reveals a New and Unexpected Domain Architecture
journal, August 2009


The molecular architecture of the nuclear pore complex
journal, November 2007

  • Alber, Frank; Dokudovskaya, Svetlana; Veenhoff, Liesbeth M.
  • Nature, Vol. 450, Issue 7170
  • DOI: 10.1038/nature06405

Proteomic analysis of the mammalian nuclear pore complex
journal, August 2002

  • Cronshaw, Janet M.; Krutchinsky, Andrew N.; Zhang, Wenzhu
  • The Journal of Cell Biology, Vol. 158, Issue 5
  • DOI: 10.1083/jcb.200206106

X-Ray Diffraction data from Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA), source of 4YCZ structure
dataset, September 2015

  • Knockenhauer, Kevin E.; Kelley, Kotaro; Schwartz, Thomas
  • SBGrid Data Bank, 8.4 G
  • DOI: 10.15785/sbgrid/175

Structural Basis of the Nic96 Subcomplex Organization in the Nuclear Pore Channel
journal, January 2008


Structural Biology of Nucleocytoplasmic Transport
journal, June 2007


Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats
journal, November 2008


Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex
journal, August 2009

  • Seo, H. -S.; Ma, Y.; Debler, E. W.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 34
  • DOI: 10.1073/pnas.0907453106

The nuclear pore complex: bridging nuclear transport and gene regulation
journal, July 2010

  • Strambio-De-Castillia, Caterina; Niepel, Mario; Rout, Michael P.
  • Nature Reviews Molecular Cell Biology, Vol. 11, Issue 7
  • DOI: 10.1038/nrm2928

UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004

  • Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
  • Journal of Computational Chemistry, Vol. 25, Issue 13
  • DOI: 10.1002/jcc.20084

The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility
journal, March 2010


A simple, versatile method for GFP-based super-resolution microscopy via nanobodies
journal, April 2012

  • Ries, Jonas; Kaplan, Charlotte; Platonova, Evgenia
  • Nature Methods, Vol. 9, Issue 6
  • DOI: 10.1038/nmeth.1991

Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009

  • Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
  • Nature Methods, Vol. 6, Issue 5, p. 343-345
  • DOI: 10.1038/nmeth.1318

Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex
journal, November 2004

  • Berke, Ian C.; Boehmer, Thomas; Blobel, Günter
  • The Journal of Cell Biology, Vol. 167, Issue 4
  • DOI: 10.1083/jcb.200408109

Structure of a trimeric nucleoporin complex reveals alternate oligomerization states
journal, October 2009

  • Nagy, V.; Hsia, K. -C.; Debler, E. W.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 42
  • DOI: 10.1073/pnas.0909373106

Architectural Nucleoporins Nup157/170 and Nup133 Are Structurally Related and Descend from a Second Ancestral Element
journal, August 2009

  • Whittle, James R. R.; Schwartz, Thomas U.
  • Journal of Biological Chemistry, Vol. 284, Issue 41
  • DOI: 10.1074/jbc.M109.023580

Comparative Genomic Evidence for a Complete Nuclear Pore Complex in the Last Eukaryotic Common Ancestor
journal, October 2010


A Tumor Suppressor Complex with GAP Activity for the Rag GTPases That Signal Amino Acid Sufficiency to mTORC1
journal, May 2013


Optimized E. coli expression strain LOBSTR eliminates common contaminants from His-tag purification : Optimized
journal, August 2013

  • Andersen, Kasper R.; Leksa, Nina C.; Schwartz, Thomas U.
  • Proteins: Structure, Function, and Bioinformatics, Vol. 81, Issue 11
  • DOI: 10.1002/prot.24364

MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009

  • Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
  • DOI: 10.1107/S0907444909042073

An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells
journal, September 2001

  • Belgareh, Naïma; Rabut, Gwénaël; Baï, Siau Wei
  • The Journal of Cell Biology, Vol. 154, Issue 6
  • DOI: 10.1083/jcb.200101081

Architecture of a Coat for the Nuclear Pore Membrane
journal, December 2007


Three-dimensional structure and flexibility of a membrane-coating module of the nuclear pore complex
journal, June 2009

  • Kampmann, Martin; Blobel, Günter
  • Nature Structural & Molecular Biology, Vol. 16, Issue 7
  • DOI: 10.1038/nsmb.1618

Atomic resolution structures in nuclear transport
journal, August 2006


Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

A Fence-like Coat for the Nuclear Pore Membrane
journal, December 2008


Structure of the C-terminal domain of Saccharomyces cerevisiae Nup133, a component of the nuclear pore complex : Structure of the Yeast Nup133 C-Terminal Domain
journal, March 2011

  • Sampathkumar, Parthasarathy; Gheyi, Tarun; Miller, Stacy A.
  • Proteins: Structure, Function, and Bioinformatics, Vol. 79, Issue 5
  • DOI: 10.1002/prot.22973

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold
journal, December 2013


Three-Dimensional Architecture of the Isolated Yeast Nuclear Pore Complex: Functional and Evolutionary Implications
journal, January 1998


Integrative Structure–Function Mapping of the Nucleoporin Nup133 Suggests a Conserved Mechanism for Membrane Anchoring of the Nuclear Pore Complex
journal, August 2014

  • Kim, Seung Joong; Fernandez-Martinez, Javier; Sampathkumar, Parthasarathy
  • Molecular & Cellular Proteomics, Vol. 13, Issue 11
  • DOI: 10.1074/mcp.M114.040915

Structure of the Sec13–Sec16 edge element, a template for assembly of the COPII vesicle coat
journal, August 2010

  • Whittle, James R. R.; Schwartz, Thomas U.
  • The Journal of Cell Biology, Vol. 190, Issue 3
  • DOI: 10.1083/jcb.201003092

A Novel Complex of Nucleoporins, Which Includes Sec13p and a Sec13p Homolog, Is Essential for Normal Nuclear Pores
journal, January 1996


Protein Interfaces of the Conserved Nup84 Complex from Chaetomium thermophilum Shown by Crosslinking Mass Spectrometry and Electron Microscopy
journal, September 2013


Crystal Structure of Nucleoporin Nic96 Reveals a Novel, Intricate Helical Domain Architecture
journal, September 2007

  • Jeudy, Sandra; Schwartz, Thomas U.
  • Journal of Biological Chemistry, Vol. 282, Issue 48
  • DOI: 10.1074/jbc.M705479200

Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins
journal, February 2002


Protein structure prediction on the Web: a case study using the Phyre server
journal, February 2009

  • Kelley, Lawrence A.; Sternberg, Michael J. E.
  • Nature Protocols, Vol. 4, Issue 3
  • DOI: 10.1038/nprot.2009.2

Border Control at the Nucleus: Biogenesis and Organization of the Nuclear Membrane and Pore Complexes
journal, November 2009


Structure–function mapping of a heptameric module in the nuclear pore complex
journal, February 2012

  • Fernandez-Martinez, Javier; Phillips, Jeremy; Sekedat, Matthew D.
  • The Journal of Cell Biology, Vol. 196, Issue 4
  • DOI: 10.1083/jcb.201109008

Architecture of the nuclear pore complex coat
journal, February 2015


Jalview Version 2--a multiple sequence alignment editor and analysis workbench
journal, January 2009


Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export
journal, October 2001

  • Vasu, Sanjay; Shah, Sundeep; Orjalo, Arturo
  • The Journal of Cell Biology, Vol. 155, Issue 3
  • DOI: 10.1083/jcb.200108007

The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism
journal, February 2000

  • Rout, Michael P.; Aitchison, John D.; Suprapto, Adisetyantari
  • The Journal of Cell Biology, Vol. 148, Issue 4
  • DOI: 10.1083/jcb.148.4.635

X-Ray Diffraction data from Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA), source of 4YCZ structure
dataset, September 2015

  • Knockenhauer, Kevin E.; Kelley, Kotaro; Schwartz, Thomas
  • SBGrid Data Bank, 8.4 G
  • DOI: 10.15785/sbgrid/175

    Works referencing / citing this record:

    X-Ray Diffraction data from Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA), source of 4YCZ structure
    dataset, September 2015

    • Knockenhauer, Kevin E.; Kelley, Kotaro; Schwartz, Thomas
    • SBGrid Data Bank, 8.4 G
    • DOI: 10.15785/sbgrid/175

    UCSF Chimera?A visualization system for exploratory research and analysis
    journal, January 2004

    • Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
    • Journal of Computational Chemistry, Vol. 25, Issue 13
    • DOI: 10.1002/jcc.20084

    Structure of the C-terminal domain of Saccharomyces cerevisiae Nup133, a component of the nuclear pore complex : Structure of the Yeast Nup133 C-Terminal Domain
    journal, March 2011

    • Sampathkumar, Parthasarathy; Gheyi, Tarun; Miller, Stacy A.
    • Proteins: Structure, Function, and Bioinformatics, Vol. 79, Issue 5
    • DOI: 10.1002/prot.22973

    Optimized E. coli expression strain LOBSTR eliminates common contaminants from His-tag purification : Optimized
    journal, August 2013

    • Andersen, Kasper R.; Leksa, Nina C.; Schwartz, Thomas U.
    • Proteins: Structure, Function, and Bioinformatics, Vol. 81, Issue 11
    • DOI: 10.1002/prot.24364

    phenix.mr_rosetta: molecular replacement and model rebuilding with Phenix and Rosetta
    journal, March 2012

    • Terwilliger, Thomas C.; DiMaio, Frank; Read, Randy J.
    • Journal of Structural and Functional Genomics, Vol. 13, Issue 2
    • DOI: 10.1007/s10969-012-9129-3

    Architecture of a Coat for the Nuclear Pore Membrane
    journal, December 2007


    The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility
    journal, March 2010


    Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold
    journal, December 2013


    Border Control at the Nucleus: Biogenesis and Organization of the Nuclear Membrane and Pore Complexes
    journal, November 2009


    Structural Basis of the Nic96 Subcomplex Organization in the Nuclear Pore Channel
    journal, January 2008


    Structural and Functional Studies of Nup107/Nup133 Interaction and Its Implications for the Architecture of the Nuclear Pore Complex
    journal, June 2008


    A Fence-like Coat for the Nuclear Pore Membrane
    journal, December 2008


    The Structure of the Scaffold Nucleoporin Nup120 Reveals a New and Unexpected Domain Architecture
    journal, August 2009


    The Nuclear Pore Complex Has Entered the Atomic Age
    journal, September 2009


    Protein Interfaces of the Conserved Nup84 Complex from Chaetomium thermophilum Shown by Crosslinking Mass Spectrometry and Electron Microscopy
    journal, September 2013


    Atomic resolution structures in nuclear transport
    journal, August 2006


    Cell type‐specific nuclear pores: a case in point for context‐dependent stoichiometry of molecular machines
    journal, January 2013

    • Ori, Alessandro; Banterle, Niccolò; Iskar, Murat
    • Molecular Systems Biology, Vol. 9, Issue 1
    • DOI: 10.1038/msb.2013.4

    The molecular architecture of the nuclear pore complex
    journal, November 2007

    • Alber, Frank; Dokudovskaya, Svetlana; Veenhoff, Liesbeth M.
    • Nature, Vol. 450, Issue 7170
    • DOI: 10.1038/nature06405

    Enzymatic assembly of DNA molecules up to several hundred kilobases
    journal, April 2009

    • Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
    • Nature Methods, Vol. 6, Issue 5, p. 343-345
    • DOI: 10.1038/nmeth.1318

    A simple, versatile method for GFP-based super-resolution microscopy via nanobodies
    journal, April 2012

    • Ries, Jonas; Kaplan, Charlotte; Platonova, Evgenia
    • Nature Methods, Vol. 9, Issue 6
    • DOI: 10.1038/nmeth.1991

    Protein structure prediction on the Web: a case study using the Phyre server
    journal, February 2009

    • Kelley, Lawrence A.; Sternberg, Michael J. E.
    • Nature Protocols, Vol. 4, Issue 3
    • DOI: 10.1038/nprot.2009.2

    The nuclear pore complex: bridging nuclear transport and gene regulation
    journal, July 2010

    • Strambio-De-Castillia, Caterina; Niepel, Mario; Rout, Michael P.
    • Nature Reviews Molecular Cell Biology, Vol. 11, Issue 7
    • DOI: 10.1038/nrm2928

    Three-dimensional structure and flexibility of a membrane-coating module of the nuclear pore complex
    journal, June 2009

    • Kampmann, Martin; Blobel, Günter
    • Nature Structural & Molecular Biology, Vol. 16, Issue 7
    • DOI: 10.1038/nsmb.1618

    Molecular architecture of the Nup84–Nup145C–Sec13 edge element in the nuclear pore complex lattice
    journal, October 2009

    • Brohawn, Stephen G.; Schwartz, Thomas U.
    • Nature Structural & Molecular Biology, Vol. 16, Issue 11
    • DOI: 10.1038/nsmb.1713

    Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex
    journal, August 2009

    • Seo, H. -S.; Ma, Y.; Debler, E. W.
    • Proceedings of the National Academy of Sciences, Vol. 106, Issue 34
    • DOI: 10.1073/pnas.0907453106

    Structure of a trimeric nucleoporin complex reveals alternate oligomerization states
    journal, October 2009

    • Nagy, V.; Hsia, K. -C.; Debler, E. W.
    • Proceedings of the National Academy of Sciences, Vol. 106, Issue 42
    • DOI: 10.1073/pnas.0909373106

    Molecular basis for Nup37 and ELY5/ELYS recruitment to the nuclear pore complex
    journal, September 2012

    • Bilokapic, S.; Schwartz, T. U.
    • Proceedings of the National Academy of Sciences, Vol. 109, Issue 38
    • DOI: 10.1073/pnas.1205151109

    Structural evolution of the membrane-coating module of the nuclear pore complex
    journal, September 2012

    • Liu, X.; Mitchell, J. M.; Wozniak, R. W.
    • Proceedings of the National Academy of Sciences, Vol. 109, Issue 41
    • DOI: 10.1073/pnas.1214557109

    The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism
    journal, February 2000

    • Rout, Michael P.; Aitchison, John D.; Suprapto, Adisetyantari
    • The Journal of Cell Biology, Vol. 148, Issue 4
    • DOI: 10.1083/jcb.148.4.635

    An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells
    journal, September 2001

    • Belgareh, Naïma; Rabut, Gwénaël; Baï, Siau Wei
    • The Journal of Cell Biology, Vol. 154, Issue 6
    • DOI: 10.1083/jcb.200101081

    Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export
    journal, October 2001

    • Vasu, Sanjay; Shah, Sundeep; Orjalo, Arturo
    • The Journal of Cell Biology, Vol. 155, Issue 3
    • DOI: 10.1083/jcb.200108007

    Proteomic analysis of the mammalian nuclear pore complex
    journal, August 2002

    • Cronshaw, Janet M.; Krutchinsky, Andrew N.; Zhang, Wenzhu
    • The Journal of Cell Biology, Vol. 158, Issue 5
    • DOI: 10.1083/jcb.200206106

    Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex
    journal, November 2004

    • Berke, Ian C.; Boehmer, Thomas; Blobel, Günter
    • The Journal of Cell Biology, Vol. 167, Issue 4
    • DOI: 10.1083/jcb.200408109

    Structure of the Sec13–Sec16 edge element, a template for assembly of the COPII vesicle coat
    journal, August 2010

    • Whittle, James R. R.; Schwartz, Thomas U.
    • The Journal of Cell Biology, Vol. 190, Issue 3
    • DOI: 10.1083/jcb.201003092

    Structure–function mapping of a heptameric module in the nuclear pore complex
    journal, February 2012

    • Fernandez-Martinez, Javier; Phillips, Jeremy; Sekedat, Matthew D.
    • The Journal of Cell Biology, Vol. 196, Issue 4
    • DOI: 10.1083/jcb.201109008

    Jalview Version 2--a multiple sequence alignment editor and analysis workbench
    journal, January 2009


    Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins
    journal, February 2002


    Toward Automatic Reconstruction of a Highly Resolved Tree of Life
    journal, March 2006


    Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats
    journal, November 2008


    A Tumor Suppressor Complex with GAP Activity for the Rag GTPases That Signal Amino Acid Sufficiency to mTORC1
    journal, May 2013


    Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging
    journal, July 2013


    Architecture of the nuclear pore complex coat
    journal, February 2015


    Structural Biology of Nucleocytoplasmic Transport
    journal, June 2007


    Comparative Genomic Evidence for a Complete Nuclear Pore Complex in the Last Eukaryotic Common Ancestor
    journal, October 2010