Fission yeast RNA triphosphatase reads an Spt5 CTD code
Abstract
mRNA capping enzymes are directed to nascent RNA polymerase II (Pol2) transcripts via interactions with the carboxy-terminal domains (CTDs) of Pol2 and transcription elongation factor Spt5. Fission yeast RNA triphosphatase binds to the Spt5 CTD, comprising a tandem repeat of nonapeptide motif TPAWNSGSK. Here we report the crystal structure of a Pct1·Spt5-CTD complex, which revealed two CTD docking sites on the Pct1 homodimer that engage TPAWN segments of the motif. Each Spt5 CTD interface, composed of elements from both subunits of the homodimer, is dominated by van der Waals contacts from Pct1 to the tryptophan of the CTD. The bound CTD adopts a distinctive conformation in which the peptide backbone makes a tight U-turn so that the proline stacks over the tryptophan. We show that Pct1 binding to Spt5 CTD is antagonized by threonine phosphorylation. Our results fortify an emerging concept of an “Spt5 CTD code” in which (i) the Spt5 CTD is structurally plastic and can adopt different conformations that are templated by particular cellular Spt5 CTD receptor proteins; and (ii) threonine phosphorylation of the Spt5 CTD repeat inscribes a binary on–off switch that is read by diverse CTD receptors, each in its own distinctive manner.
- Authors:
-
- Sloan-Kettering Inst., New York, NY (United States). Structural Biology Program
- Weill Cornell Medical College, New York, NY (United States). Microbiology and Immunology Dept.
- Sloan-Kettering Inst., New York, NY (United States). Molecular Biology Program
- Sloan-Kettering Inst., New York, NY (United States). Structural Biology Program. Howard Hughes Medical Inst.
- Publication Date:
- Research Org.:
- Sloan-Kettering Inst., New York, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health (NIH) (United States)
- Contributing Org.:
- Weill Cornell Medical College, New York, NY (United States)
- OSTI Identifier:
- 1168505
- Grant/Contract Number:
- AC02-06CH11357; RR-15301; GM061906; GM052470
- Resource Type:
- Accepted Manuscript
- Journal Name:
- RNA
- Additional Journal Information:
- Journal Volume: 21; Journal Issue: 1; Journal ID: ISSN 1355-8382
- Publisher:
- Cambridge University Press
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; transcription; elongation; mRNA processing; mRNA capping; X-ray crystallography
Citation Formats
Doamekpor, Selom K., Schwer, Beate, Sanchez, Ana M., Shuman, Stewart, and Lima, Christopher D. Fission yeast RNA triphosphatase reads an Spt5 CTD code. United States: N. p., 2014.
Web. doi:10.1261/rna.048181.114.
Doamekpor, Selom K., Schwer, Beate, Sanchez, Ana M., Shuman, Stewart, & Lima, Christopher D. Fission yeast RNA triphosphatase reads an Spt5 CTD code. United States. https://doi.org/10.1261/rna.048181.114
Doamekpor, Selom K., Schwer, Beate, Sanchez, Ana M., Shuman, Stewart, and Lima, Christopher D. Thu .
"Fission yeast RNA triphosphatase reads an Spt5 CTD code". United States. https://doi.org/10.1261/rna.048181.114. https://www.osti.gov/servlets/purl/1168505.
@article{osti_1168505,
title = {Fission yeast RNA triphosphatase reads an Spt5 CTD code},
author = {Doamekpor, Selom K. and Schwer, Beate and Sanchez, Ana M. and Shuman, Stewart and Lima, Christopher D.},
abstractNote = {mRNA capping enzymes are directed to nascent RNA polymerase II (Pol2) transcripts via interactions with the carboxy-terminal domains (CTDs) of Pol2 and transcription elongation factor Spt5. Fission yeast RNA triphosphatase binds to the Spt5 CTD, comprising a tandem repeat of nonapeptide motif TPAWNSGSK. Here we report the crystal structure of a Pct1·Spt5-CTD complex, which revealed two CTD docking sites on the Pct1 homodimer that engage TPAWN segments of the motif. Each Spt5 CTD interface, composed of elements from both subunits of the homodimer, is dominated by van der Waals contacts from Pct1 to the tryptophan of the CTD. The bound CTD adopts a distinctive conformation in which the peptide backbone makes a tight U-turn so that the proline stacks over the tryptophan. We show that Pct1 binding to Spt5 CTD is antagonized by threonine phosphorylation. Our results fortify an emerging concept of an “Spt5 CTD code” in which (i) the Spt5 CTD is structurally plastic and can adopt different conformations that are templated by particular cellular Spt5 CTD receptor proteins; and (ii) threonine phosphorylation of the Spt5 CTD repeat inscribes a binary on–off switch that is read by diverse CTD receptors, each in its own distinctive manner.},
doi = {10.1261/rna.048181.114},
journal = {RNA},
number = 1,
volume = 21,
place = {United States},
year = {Thu Nov 20 00:00:00 EST 2014},
month = {Thu Nov 20 00:00:00 EST 2014}
}
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Figures / Tables:
FIGURE 1: Structure of the Pct1·Spt5 complex. (A) The structure of the Pct1 homodimer is depicted as a cartoon model with the A and B protomers colored green and cyan, respectively. The two Spt5 CTD petides are rendered as stick models with gray carbons. (B) Surface view of the Pct1more »
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Works referencing / citing this record:
Interplay of mRNA capping and transcription machineries
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Functional interaction of Rpb1 and Spt5 C-terminal domains in co-transcriptional histone modification
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