Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids

Takimoto, Jeffrey K.; Dellas, Nikki; Noel, Joseph P.; Wang, Lei

doi:10.1021/cb200057a

Title: Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids

Authors:

Takimoto, Jeffrey K. ^[1]; Dellas, Nikki ^[1]; Noel, Joseph P. ^[2]; Wang, Lei ^[1]

The Jack H. Skirball Center for Chemical Biology & Proteomics
The Jack H. Skirball Center for Chemical Biology & Proteomics, Howard Hughes Medical Institute, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, United States

Publication Date:: Thu May 05 00:00:00 EDT 2011

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1123355

Resource Type:: Published Article

Journal Name:: ACS Chemical Biology

Additional Journal Information:: Journal Name: ACS Chemical Biology Journal Volume: 6 Journal Issue: 7; Journal ID: ISSN 1554-8929

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Citation Formats


                    Takimoto, Jeffrey K., Dellas, Nikki, Noel, Joseph P., and Wang, Lei. Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids.  United States: N. p., 2011. 
Web.  doi:10.1021/cb200057a.

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                    Takimoto, Jeffrey K., Dellas, Nikki, Noel, Joseph P., & Wang, Lei. Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids.  United States.  https://doi.org/10.1021/cb200057a

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                    Takimoto, Jeffrey K., Dellas, Nikki, Noel, Joseph P., and Wang, Lei. Thu .  
"Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids".  United States.  https://doi.org/10.1021/cb200057a.

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@article{osti_1123355,

  title        = {Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids},

  author       = {Takimoto, Jeffrey K. and Dellas, Nikki and Noel, Joseph P. and Wang, Lei},

  abstractNote = {},

  doi          = {10.1021/cb200057a},

  journal      = {ACS Chemical Biology},

  number       = 7,

  volume       = 6,

  place        = {United States},

  year         = {Thu May 05 00:00:00 EDT 2011},

  month        = {Thu May 05 00:00:00 EDT 2011}

}

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Title: Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids

Citation Formats

Pyrrolysine is not hardwired for cotranslational insertion at UAG codons journal, February 2007

Direct charging of tRNACUA with pyrrolysine in vitro and in vivo journal, August 2004

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion journal, January 2005

Genetic incorporation of unnatural amino acids into proteins in mammalian cells journal, February 2007

Addition of the keto functional group to the genetic code of Escherichia coli journal, December 2002

Pyrrolysine analogues as substrates for pyrrolysyl-tRNA synthetase journal, November 2006

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases journal, July 2008

Structural plasticity of an aminoacyl-tRNA synthetase active site journal, April 2006

Specificity of Pyrrolysyl-tRNA Synthetase for Pyrrolysine and Pyrrolysine Analogs journal, January 2009

Expanding the Genetic Code of Escherichia coli journal, April 2001

Pyrrolysine Encoded by UAG in Archaea: Charging of a UAG-Decoding Specialized tRNA journal, May 2002

A genetically encoded photocaged Nε-methyl-l-lysine journal, January 2010

The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives journal, January 2011

The Orbitrap: a new mass spectrometer journal, January 2005

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli journal, January 2010

Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination journal, September 1998

Aminoacyl-tRNA Synthesis journal, June 2000

Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality journal, December 2008

PROCHECK: a program to check the stereochemical quality of protein structures journal, April 1993

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast journal, May 2008

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification journal, November 2008

A general approach for the generation of orthogonal tRNAs journal, September 2001

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase journal, May 2008

Adding New Chemistries to the Genetic Code journal, June 2010

Genetically encoding unnatural amino acids for cellular and neuronal studies journal, July 2007

An Expanded Eukaryotic Genetic Code journal, August 2003

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells journal, January 2009

Expanding the Genetic Code journal, January 2005

The CCP4 suite programs for protein crystallography journal, September 1994

Expanding the Genetic Code for Biological Studies journal, March 2009

Structure and activity of an aminoacyl-tRNA synthetase that charges tRNA with nitro-tryptophan journal, February 2005

Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation journal, June 2007

A genetically encoded fluorescent amino acid journal, June 2006

Adding pyrrolysine to the Escherichia coli genetic code journal, October 2007

A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells journal, May 2009

Synthesis of Glu-tRNA Gln by Engineered and Natural Aminoacyl-tRNA Synthetases journal, August 2010

Indirect Readout of tRNA for Aminoacylation journal, August 2007

Pyrrolysine is not hardwired for cotranslational insertion at UAG codons
journal, February 2007

Direct charging of tRNA_CUA with pyrrolysine in vitro and in vivo
journal, August 2004

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion
journal, January 2005

Genetic incorporation of unnatural amino acids into proteins in mammalian cells
journal, February 2007

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002

Pyrrolysine analogues as substrates for pyrrolysyl-tRNA synthetase
journal, November 2006

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases
journal, July 2008

Structural plasticity of an aminoacyl-tRNA synthetase active site
journal, April 2006

Specificity of Pyrrolysyl-tRNA Synthetase for Pyrrolysine and Pyrrolysine Analogs
journal, January 2009

Expanding the Genetic Code of Escherichia coli
journal, April 2001

Pyrrolysine Encoded by UAG in Archaea: Charging of a UAG-Decoding Specialized tRNA
journal, May 2002

A genetically encoded photocaged Nε-methyl-l-lysine
journal, January 2010

The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives
journal, January 2011

The Orbitrap: a new mass spectrometer
journal, January 2005

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli
journal, January 2010

Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
journal, September 1998

Aminoacyl-tRNA Synthesis
journal, June 2000

Pyrrolysyl-tRNA synthetase–tRNA^Pyl structure reveals the molecular basis of orthogonality
journal, December 2008

PROCHECK: a program to check the stereochemical quality of protein structures
journal, April 1993

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast
journal, May 2008

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification
journal, November 2008

A general approach for the generation of orthogonal tRNAs
journal, September 2001

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase
journal, May 2008

Adding New Chemistries to the Genetic Code
journal, June 2010

Genetically encoding unnatural amino acids for cellular and neuronal studies
journal, July 2007

An Expanded Eukaryotic Genetic Code
journal, August 2003

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells
journal, January 2009

Expanding the Genetic Code
journal, January 2005

The CCP4 suite programs for protein crystallography
journal, September 1994

Expanding the Genetic Code for Biological Studies
journal, March 2009

Structure and activity of an aminoacyl-tRNA synthetase that charges tRNA with nitro-tryptophan
journal, February 2005

Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation
journal, June 2007

A genetically encoded fluorescent amino acid
journal, June 2006

Adding pyrrolysine to the Escherichia coli genetic code
journal, October 2007

A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells
journal, May 2009

Synthesis of Glu-tRNA ^Gln by Engineered and Natural Aminoacyl-tRNA Synthetases
journal, August 2010

Indirect Readout of tRNA for Aminoacylation
journal, August 2007