You need JavaScript to view this

Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid

Abstract

Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid have been performed with a view to producing optimal assay conditions. Cholic acid-BSA was found to be the best immunogen to produce antibodies to conjugated cholic acid and the response was of an IgG type. Incorporating a spacer (hexanoic acid) between hapten and carrier protein resulted in a decrease in antiserum titre. Optimal conditions for the assay were found using (/sup 125/I)histamine-glycocholic acid as ligand with a dilution of antiserum to produce 60% binding of ligand and a pH of 7.4. Using these assay conditions no serum effects were found; extraction of serum prior to assay was therefore unnecessary. The assay was sensitive enough to detect post-prandial increases in serum bile acid concentrations following a liquid test meal; no increase was observed throughout the same time period in a fasting control.
Authors:
Beckett, G J; Percy-Robb, I W; [1]  Hunter, W M [2] 
  1. Royal Infirmary, Edinburgh (UK)
  2. Medical Research Council, Edinburgh (UK)
Publication Date:
Sep 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-423526; EDB-79-101349
Resource Relation:
Journal Name: Clin. Chim. Acta; (Netherlands); Journal Volume: 88:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CHOLIC ACID; RADIOIMMUNOASSAY; OPTIMIZATION; ACCURACY; ANTIBODIES; HEXANOIC ACID; HISTAMINE; IMMUNE SERUMS; IODINE 125; LIGANDS; PH VALUE; RABBITS; SENSITIVITY; SPECIFICITY; TRITIUM; AMINES; ANIMALS; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BILE ACIDS; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; HYDROXY COMPOUNDS; IMIDAZOLES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MONOCARBOXYLIC ACIDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIOASSAY; RADIOISOTOPES; STEROIDS; STEROLS; TRACER TECHNIQUES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6133353
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CCATA
Submitting Site:
INIS
Size:
Pages: 257-266
Announcement Date:

Citation Formats

Beckett, G J, Percy-Robb, I W, and Hunter, W M. Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid. Netherlands: N. p., 1978. Web. doi:10.1016/0009-8981(78)90430-8.
Beckett, G J, Percy-Robb, I W, & Hunter, W M. Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid. Netherlands. doi:10.1016/0009-8981(78)90430-8.
Beckett, G J, Percy-Robb, I W, and Hunter, W M. 1978. "Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid." Netherlands. doi:10.1016/0009-8981(78)90430-8. https://www.osti.gov/servlets/purl/10.1016/0009-8981(78)90430-8.
@misc{etde_6133353,
title = {Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid}
author = {Beckett, G J, Percy-Robb, I W, and Hunter, W M}
abstractNote = {Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid have been performed with a view to producing optimal assay conditions. Cholic acid-BSA was found to be the best immunogen to produce antibodies to conjugated cholic acid and the response was of an IgG type. Incorporating a spacer (hexanoic acid) between hapten and carrier protein resulted in a decrease in antiserum titre. Optimal conditions for the assay were found using (/sup 125/I)histamine-glycocholic acid as ligand with a dilution of antiserum to produce 60% binding of ligand and a pH of 7.4. Using these assay conditions no serum effects were found; extraction of serum prior to assay was therefore unnecessary. The assay was sensitive enough to detect post-prandial increases in serum bile acid concentrations following a liquid test meal; no increase was observed throughout the same time period in a fasting control.}
doi = {10.1016/0009-8981(78)90430-8}
journal = {Clin. Chim. Acta; (Netherlands)}
volume = {88:2}
journal type = {AC}
place = {Netherlands}
year = {1978}
month = {Sep}
}