Abstract
Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid have been performed with a view to producing optimal assay conditions. Cholic acid-BSA was found to be the best immunogen to produce antibodies to conjugated cholic acid and the response was of an IgG type. Incorporating a spacer (hexanoic acid) between hapten and carrier protein resulted in a decrease in antiserum titre. Optimal conditions for the assay were found using (/sup 125/I)histamine-glycocholic acid as ligand with a dilution of antiserum to produce 60% binding of ligand and a pH of 7.4. Using these assay conditions no serum effects were found; extraction of serum prior to assay was therefore unnecessary. The assay was sensitive enough to detect post-prandial increases in serum bile acid concentrations following a liquid test meal; no increase was observed throughout the same time period in a fasting control.
Beckett, G J;
Percy-Robb, I W;
[1]
Hunter, W M
[2]
- Royal Infirmary, Edinburgh (UK)
- Medical Research Council, Edinburgh (UK)
Citation Formats
Beckett, G J, Percy-Robb, I W, and Hunter, W M.
Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid.
Netherlands: N. p.,
1978.
Web.
doi:10.1016/0009-8981(78)90430-8.
Beckett, G J, Percy-Robb, I W, & Hunter, W M.
Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid.
Netherlands.
https://doi.org/10.1016/0009-8981(78)90430-8
Beckett, G J, Percy-Robb, I W, and Hunter, W M.
1978.
"Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid."
Netherlands.
https://doi.org/10.1016/0009-8981(78)90430-8.
@misc{etde_6133353,
title = {Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid}
author = {Beckett, G J, Percy-Robb, I W, and Hunter, W M}
abstractNote = {Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid have been performed with a view to producing optimal assay conditions. Cholic acid-BSA was found to be the best immunogen to produce antibodies to conjugated cholic acid and the response was of an IgG type. Incorporating a spacer (hexanoic acid) between hapten and carrier protein resulted in a decrease in antiserum titre. Optimal conditions for the assay were found using (/sup 125/I)histamine-glycocholic acid as ligand with a dilution of antiserum to produce 60% binding of ligand and a pH of 7.4. Using these assay conditions no serum effects were found; extraction of serum prior to assay was therefore unnecessary. The assay was sensitive enough to detect post-prandial increases in serum bile acid concentrations following a liquid test meal; no increase was observed throughout the same time period in a fasting control.}
doi = {10.1016/0009-8981(78)90430-8}
journal = []
volume = {88:2}
journal type = {AC}
place = {Netherlands}
year = {1978}
month = {Sep}
}
title = {Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid}
author = {Beckett, G J, Percy-Robb, I W, and Hunter, W M}
abstractNote = {Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid have been performed with a view to producing optimal assay conditions. Cholic acid-BSA was found to be the best immunogen to produce antibodies to conjugated cholic acid and the response was of an IgG type. Incorporating a spacer (hexanoic acid) between hapten and carrier protein resulted in a decrease in antiserum titre. Optimal conditions for the assay were found using (/sup 125/I)histamine-glycocholic acid as ligand with a dilution of antiserum to produce 60% binding of ligand and a pH of 7.4. Using these assay conditions no serum effects were found; extraction of serum prior to assay was therefore unnecessary. The assay was sensitive enough to detect post-prandial increases in serum bile acid concentrations following a liquid test meal; no increase was observed throughout the same time period in a fasting control.}
doi = {10.1016/0009-8981(78)90430-8}
journal = []
volume = {88:2}
journal type = {AC}
place = {Netherlands}
year = {1978}
month = {Sep}
}