"TITLE","AUTHORS","SUBJECT","SUBJECT_RELATED","DESCRIPTION","PUBLISHER","AVAILABILITY","RESEARCH_ORG","SPONSORING_ORG","PUBLICATION_COUNTRY","PUBLICATION_DATE","CONTRIBUTING_ORGS","LANGUAGE","RESOURCE_TYPE","TYPE_QUALIFIER","JOURNAL_ISSUE","JOURNAL_VOLUME","RELATION","COVERAGE","FORMAT","IDENTIFIER","REPORT_NUMBER","DOE_CONTRACT_NUMBER","OTHER_IDENTIFIER","DOI","RIGHTS","ENTRY_DATE","OSTI_IDENTIFIER","PURL_URL" "Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid","Beckett, G J; Percy-Robb, I W [Royal Infirmary, Edinburgh (UK)]; Hunter, W M [Medical Research Council, Edinburgh (UK)]","62 RADIOLOGY AND NUCLEAR MEDICINE; CHOLIC ACID; RADIOIMMUNOASSAY; OPTIMIZATION; ACCURACY; ANTIBODIES; HEXANOIC ACID; HISTAMINE; IMMUNE SERUMS; IODINE 125; LIGANDS; PH VALUE; RABBITS; SENSITIVITY; SPECIFICITY; TRITIUM; AMINES; ANIMALS; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BILE ACIDS; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; HYDROXY COMPOUNDS; IMIDAZOLES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MONOCARBOXYLIC ACIDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIOASSAY; RADIOISOTOPES; STEROIDS; STEROLS; TRACER TECHNIQUES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics","","Investigations into the choice of immunogen, ligand, antiserum and assay conditions for the radioimmunoassay of conjugated cholic acid have been performed with a view to producing optimal assay conditions. Cholic acid-BSA was found to be the best immunogen to produce antibodies to conjugated cholic acid and the response was of an IgG type. Incorporating a spacer (hexanoic acid) between hapten and carrier protein resulted in a decrease in antiserum titre. Optimal conditions for the assay were found using (/sup 125/I)histamine-glycocholic acid as ligand with a dilution of antiserum to produce 60% binding of ligand and a pH of 7.4. Using these assay conditions no serum effects were found; extraction of serum prior to assay was therefore unnecessary. The assay was sensitive enough to detect post-prandial increases in serum bile acid concentrations following a liquid test meal; no increase was observed throughout the same time period in a fasting control.","","","","","Netherlands","1978-09-01","","English","Journal Article","","","88:2","Journal Name: Clin. Chim. Acta; (Netherlands); Journal Volume: 88:2","","Medium: X; Size: Pages: 257-266","","","","Journal ID: CODEN: CCATA","https://doi.org/10.1016/0009-8981(78)90430-8","","2010-12-30","6133353",""