Abstract
In order to study the effects of {sup 6} {sup 0} Co gamma radiation on crystalline proteins an in vitro system was set up. For that, aqueous solutions from bovine crystalline were used irradiated with 0, 5.000, 10.000, 15.000, 20.000 and 25.000 Gy. The treatment led to protein alterations determined by different methods. By turbidimetry the formation of aggregates that increased with the radiation dose was revealed. The same observation was done from viscosity data and from the UV spectrum of the samples. From amino acid analysis and fluorimetry determinations, tryptophan appeared as the most sensitive amino acid. An increase in the free-S H-groups was also observed. After the standardization of the method, the radio modifier capability of glutathione, amino ethyl thiourea, mercapto ethyl alanine and dimethyl sulfoxide was tested. The results showed that in the presence of those substances the radiation effect was diminished. (author).
Citation Formats
Bernardes, D M.L.
Effect of {sup 60} Co gamma radiation on crystalline proteins; Efeito da radiacao gama de cobalto 60 nas proteinas do cristalino.
Brazil: N. p.,
1991.
Web.
Bernardes, D M.L.
Effect of {sup 60} Co gamma radiation on crystalline proteins; Efeito da radiacao gama de cobalto 60 nas proteinas do cristalino.
Brazil.
Bernardes, D M.L.
1991.
"Effect of {sup 60} Co gamma radiation on crystalline proteins; Efeito da radiacao gama de cobalto 60 nas proteinas do cristalino."
Brazil.
@misc{etde_10191351,
title = {Effect of {sup 60} Co gamma radiation on crystalline proteins; Efeito da radiacao gama de cobalto 60 nas proteinas do cristalino}
author = {Bernardes, D M.L.}
abstractNote = {In order to study the effects of {sup 6} {sup 0} Co gamma radiation on crystalline proteins an in vitro system was set up. For that, aqueous solutions from bovine crystalline were used irradiated with 0, 5.000, 10.000, 15.000, 20.000 and 25.000 Gy. The treatment led to protein alterations determined by different methods. By turbidimetry the formation of aggregates that increased with the radiation dose was revealed. The same observation was done from viscosity data and from the UV spectrum of the samples. From amino acid analysis and fluorimetry determinations, tryptophan appeared as the most sensitive amino acid. An increase in the free-S H-groups was also observed. After the standardization of the method, the radio modifier capability of glutathione, amino ethyl thiourea, mercapto ethyl alanine and dimethyl sulfoxide was tested. The results showed that in the presence of those substances the radiation effect was diminished. (author).}
place = {Brazil}
year = {1991}
month = {Dec}
}
title = {Effect of {sup 60} Co gamma radiation on crystalline proteins; Efeito da radiacao gama de cobalto 60 nas proteinas do cristalino}
author = {Bernardes, D M.L.}
abstractNote = {In order to study the effects of {sup 6} {sup 0} Co gamma radiation on crystalline proteins an in vitro system was set up. For that, aqueous solutions from bovine crystalline were used irradiated with 0, 5.000, 10.000, 15.000, 20.000 and 25.000 Gy. The treatment led to protein alterations determined by different methods. By turbidimetry the formation of aggregates that increased with the radiation dose was revealed. The same observation was done from viscosity data and from the UV spectrum of the samples. From amino acid analysis and fluorimetry determinations, tryptophan appeared as the most sensitive amino acid. An increase in the free-S H-groups was also observed. After the standardization of the method, the radio modifier capability of glutathione, amino ethyl thiourea, mercapto ethyl alanine and dimethyl sulfoxide was tested. The results showed that in the presence of those substances the radiation effect was diminished. (author).}
place = {Brazil}
year = {1991}
month = {Dec}
}