Effect of {sup 60} Co gamma radiation on crystalline proteins; Efeito da radiacao gama de cobalto 60 nas proteinas do cristalino Bernardes, D M.L. 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; PROTEINS; CHEMICAL RADIATION EFFECTS; CRYSTALLINE LENS; COBALT 60; GAMMA RADIATION; RADIATION DOSES; RADIOPROTECTIVE SUBSTANCES; 560120; 400600; RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE; RADIATION CHEMISTRY In order to study the effects of {sup 6} {sup 0} Co gamma radiation on crystalline proteins an in vitro system was set up. For that, aqueous solutions from bovine crystalline were used irradiated with 0, 5.000, 10.000, 15.000, 20.000 and 25.000 Gy. The treatment led to protein alterations determined by different methods. By turbidimetry the formation of aggregates that increased with the radiation dose was revealed. The same observation was done from viscosity data and from the UV spectrum of the samples. From amino acid analysis and fluorimetry determinations, tryptophan appeared as the most sensitive amino acid. An increase in the free-S H-groups was also observed. After the standardization of the method, the radio modifier capability of glutathione, amino ethyl thiourea, mercapto ethyl alanine and dimethyl sulfoxide was tested. The results showed that in the presence of those substances the radiation effect was diminished. (author). Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil) OSTI; NTIS (US Sales Only); INIS Brazil 1992-12-31 Portuguese Thesis/Dissertation Miscellaneous Other Information: TH: Tese (M.Sc.).; PBD: 1991 Medium: X; Size: 67 p. https://doi.org/ ON: DE93607199 [] INIS-BR-2986 Other: ON: DE93607199; TRN: BR9230224086252 INIS; SCA: 560120; 400600; PA: AIX-23:086252; SN: 92000885673 2008-02-12 10191351