Ellipsoidal cell flow system
Abstract
The disclosure relates to a system incorporating an ellipsoidal flow chamber having light reflective walls for low level light detection in practicing cellular analysis. The system increases signal-to-noise ratio by a factor of ten over prior art systems. In operation, laser light passes through the primary focus of the ellipsoid. A controlled flow of cells simultaneously passes through this focus so that the laser light impinges on the cells and is modulated by the cells. The reflective walls of the ellipsoid reflect the cell-modulated light to the secondary focus of the ellipsoid. A tapered light guide at the secondary focus picks up a substantial portion of modulated reflective light and directs it onto a light detector to produce a signal. The signal is processed to obtain the intensity distribution of the modulated light and hence sought after characteristics of the cells. In addition, cells may be dyed so as to fluoresce in response to the laser light and their fluorescence may be processed as cell-modulated light above described. A light discriminating filter would be used to distinguish reflected modulated laser light from reflected fluorescent light.
- Inventors:
-
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 862483
- Patent Number(s):
- 3946239
- Assignee:
- United States of America as represented by United Energy (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- ellipsoidal; cell; flow; disclosure; relates; incorporating; chamber; light; reflective; walls; level; detection; practicing; cellular; analysis; increases; signal-to-noise; ratio; factor; prior; systems; operation; laser; passes; primary; focus; ellipsoid; controlled; cells; simultaneously; impinges; modulated; reflect; cell-modulated; secondary; tapered; guide; picks; substantial; portion; directs; detector; produce; signal; processed; obtain; intensity; distribution; hence; sought; characteristics; addition; dyed; fluoresce; response; fluorescence; described; discriminating; filter; distinguish; reflected; fluorescent; light passes; substantial portion; fluorescent light; signal-to-noise ratio; laser light; disclosure relates; light guide; flow chamber; reflective wall; light detector; intensity distribution; noise ratio; modulated laser; modulated light; controlled flow; light detection; /250/356/
Citation Formats
Salzman, Gary C, and Mullaney, Paul F. Ellipsoidal cell flow system. United States: N. p., 1976.
Web.
Salzman, Gary C, & Mullaney, Paul F. Ellipsoidal cell flow system. United States.
Salzman, Gary C, and Mullaney, Paul F. Thu .
"Ellipsoidal cell flow system". United States. https://www.osti.gov/servlets/purl/862483.
@article{osti_862483,
title = {Ellipsoidal cell flow system},
author = {Salzman, Gary C and Mullaney, Paul F},
abstractNote = {The disclosure relates to a system incorporating an ellipsoidal flow chamber having light reflective walls for low level light detection in practicing cellular analysis. The system increases signal-to-noise ratio by a factor of ten over prior art systems. In operation, laser light passes through the primary focus of the ellipsoid. A controlled flow of cells simultaneously passes through this focus so that the laser light impinges on the cells and is modulated by the cells. The reflective walls of the ellipsoid reflect the cell-modulated light to the secondary focus of the ellipsoid. A tapered light guide at the secondary focus picks up a substantial portion of modulated reflective light and directs it onto a light detector to produce a signal. The signal is processed to obtain the intensity distribution of the modulated light and hence sought after characteristics of the cells. In addition, cells may be dyed so as to fluoresce in response to the laser light and their fluorescence may be processed as cell-modulated light above described. A light discriminating filter would be used to distinguish reflected modulated laser light from reflected fluorescent light.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1976},
month = {Thu Jan 01 00:00:00 EST 1976}
}