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Title: Polypeptide having cellobiohydrolase activity and uses thereof

Abstract

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Inventors:
; ; ;
Issue Date:
Research Org.:
DSM IP ASSETS B.V., Heerlen (Netherlands)
Sponsoring Org.:
USDOE
OSTI Identifier:
1215543
Patent Number(s):
9133448
Application Number:
13/578,223
Assignee:
DSM IP ASSETS B.V. (Heerlen, NL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
DOE Contract Number:  
FC36-08GO18079
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Feb 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Sagt, Cornelis Maria Jacobus, Schooneveld-Bergmans, Margot Elisabeth Francoise, Roubos, Johannes Andries, and Los, Alrik Pieter. Polypeptide having cellobiohydrolase activity and uses thereof. United States: N. p., 2015. Web.
Sagt, Cornelis Maria Jacobus, Schooneveld-Bergmans, Margot Elisabeth Francoise, Roubos, Johannes Andries, & Los, Alrik Pieter. Polypeptide having cellobiohydrolase activity and uses thereof. United States.
Sagt, Cornelis Maria Jacobus, Schooneveld-Bergmans, Margot Elisabeth Francoise, Roubos, Johannes Andries, and Los, Alrik Pieter. Tue . "Polypeptide having cellobiohydrolase activity and uses thereof". United States. https://www.osti.gov/servlets/purl/1215543.
@article{osti_1215543,
title = {Polypeptide having cellobiohydrolase activity and uses thereof},
author = {Sagt, Cornelis Maria Jacobus and Schooneveld-Bergmans, Margot Elisabeth Francoise and Roubos, Johannes Andries and Los, Alrik Pieter},
abstractNote = {The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 15 00:00:00 EDT 2015},
month = {Tue Sep 15 00:00:00 EDT 2015}
}

Works referenced in this record:

Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
patent, January 2012


Method for treating cellulosic material and CBHII/CEL6A enzymes useful therein
patent, November 2013


Modified Family 6 Glycosidases with Altered Substrate Specificity
patent-application, January 2010


Talaromyces Transformants
patent-application, November 2012


Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 Å resolution
journal, January 1999


Correlation between cellulose binding and activity of cellulose-binding domain mutants of Humicola grisea cellobiohydrolase 1
journal, December 2007