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Title: Method of inactivation of an end product of energy metabolism in Zymomonas mobilis

Abstract

The present invention briefly provides a method of site-specific insertion in Zymomonas, comprising, providing a Zymomonas gene fragment, interrupting a DNA sequence the fragment, and transforming the Zymomonas through homologous recombination with the interrupted fragment.

Inventors:
 [1];  [1]
  1. Lakewood, CO
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Midwest Research Institute, Kansas City, MO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
984338
Patent Number(s):
7374939
Application Number:
09/562,613
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhang, Min, and Chou, Yat-Chen. Method of inactivation of an end product of energy metabolism in Zymomonas mobilis. United States: N. p., 2008. Web.
Zhang, Min, & Chou, Yat-Chen. Method of inactivation of an end product of energy metabolism in Zymomonas mobilis. United States.
Zhang, Min, and Chou, Yat-Chen. Tue . "Method of inactivation of an end product of energy metabolism in Zymomonas mobilis". United States. https://www.osti.gov/servlets/purl/984338.
@article{osti_984338,
title = {Method of inactivation of an end product of energy metabolism in Zymomonas mobilis},
author = {Zhang, Min and Chou, Yat-Chen},
abstractNote = {The present invention briefly provides a method of site-specific insertion in Zymomonas, comprising, providing a Zymomonas gene fragment, interrupting a DNA sequence the fragment, and transforming the Zymomonas through homologous recombination with the interrupted fragment.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {5}
}

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Works referenced in this record:

Transposon mutagenesis and strain construction in Zymomonas mobilis
journal, March 1997


Structural Analysis of Tn5
journal, January 1981


The IdhA Gene Encoding the Fermentative Lactate Dehydrogenase of Escherichia Coli
journal, January 1997


Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995


Factors affecting transposition activity of IS50 and Tn5 ends
journal, March 1989


Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering.
journal, January 1996


Conditions that promote production of lactic acid byZymomonas mobilis in batch and continuous culture
journal, March 1998


Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli.
journal, January 1993