Aptamers and methods for their in vitro selection and uses thereof
Abstract
The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.
- Inventors:
-
- Walnut Creek, CA
- Severna Park, MD
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 983907
- Patent Number(s):
- 7329742
- Application Number:
- s 10/934,856
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Doyle, Sharon A, and Murphy, Michael B. Aptamers and methods for their in vitro selection and uses thereof. United States: N. p., 2008.
Web.
Doyle, Sharon A, & Murphy, Michael B. Aptamers and methods for their in vitro selection and uses thereof. United States.
Doyle, Sharon A, and Murphy, Michael B. Tue .
"Aptamers and methods for their in vitro selection and uses thereof". United States. https://www.osti.gov/servlets/purl/983907.
@article{osti_983907,
title = {Aptamers and methods for their in vitro selection and uses thereof},
author = {Doyle, Sharon A and Murphy, Michael B},
abstractNote = {The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 12 00:00:00 EST 2008},
month = {Tue Feb 12 00:00:00 EST 2008}
}
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