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Title: Aptamers and methods for their in vitro selection and uses thereof

Abstract

The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.

Inventors:
 [1];  [2]
  1. Walnut Creek, CA
  2. Severna Park, MD
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
983907
Patent Number(s):
7329742
Application Number:
s 10/934,856
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Doyle, Sharon A, and Murphy, Michael B. Aptamers and methods for their in vitro selection and uses thereof. United States: N. p., 2008. Web.
Doyle, Sharon A, & Murphy, Michael B. Aptamers and methods for their in vitro selection and uses thereof. United States.
Doyle, Sharon A, and Murphy, Michael B. Tue . "Aptamers and methods for their in vitro selection and uses thereof". United States. https://www.osti.gov/servlets/purl/983907.
@article{osti_983907,
title = {Aptamers and methods for their in vitro selection and uses thereof},
author = {Doyle, Sharon A and Murphy, Michael B},
abstractNote = {The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {2}
}

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Works referenced in this record:

Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide
journal, February 2002


Aptamer affinity chromatography:
journal, August 1999


Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures
journal, February 1992


In vitro selection of RNA molecules that bind specific ligands
journal, August 1990


High-throughput protein arrays: prospects for molecular diagnostics
journal, June 2002