Aptamers and methods for their in vitro selection and uses thereof
Abstract
The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.
- Inventors:
-
- Walnut Creek, CA
- Severna Park, MD
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1034609
- Patent Number(s):
- 8105982
- Application Number:
- 12/027,239
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48; AC03-76SF00098; W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Doyle, Sharon A, and Murphy, Michael B. Aptamers and methods for their in vitro selection and uses thereof. United States: N. p., 2012.
Web.
Doyle, Sharon A, & Murphy, Michael B. Aptamers and methods for their in vitro selection and uses thereof. United States.
Doyle, Sharon A, and Murphy, Michael B. Tue .
"Aptamers and methods for their in vitro selection and uses thereof". United States. https://www.osti.gov/servlets/purl/1034609.
@article{osti_1034609,
title = {Aptamers and methods for their in vitro selection and uses thereof},
author = {Doyle, Sharon A and Murphy, Michael B},
abstractNote = {The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {1}
}
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Works referenced in this record:
Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide
journal, February 2002
- Ylera, Francisco; Lurz, Rudi; Erdmann, Volker A.
- Biochemical and Biophysical Research Communications, Vol. 290, Issue 5
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase
journal, August 1990
- Tuerk, C.; Gold, L.
- Science, Vol. 249, Issue 4968
Aptamer affinity chromatography:
journal, August 1999
- Romig, Timothy S.; Bell, Carol; Drolet, Daniel W.
- Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 731, Issue 2
Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures
journal, February 1992
- Ellington, Andrew D.; Szostak, Jack W.
- Nature, Vol. 355, Issue 6363
Automated Acquisition of Aptamer Sequences
journal, June 2002
- Cox, J. Colin; Rajendran, Manjula; Riedel, Timothy
- Combinatorial Chemistry & High Throughput Screening, Vol. 5, Issue 4, p. 289-299
In vitro selection of RNA molecules that bind specific ligands
journal, August 1990
- Ellington, Andrew D.; Szostak, Jack W.
- Nature, Vol. 346, Issue 6287
Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer
journal, October 2002
- Cox, J. C.
- Nucleic Acids Research, Vol. 30, Issue 20
High-throughput protein arrays: prospects for molecular diagnostics
journal, June 2002
- Walter, Gerald; Büssow, Konrad; Lueking, Angelika
- Trends in Molecular Medicine, Vol. 8, Issue 6