Integrated solid-state rapid thermo-cycling system
Abstract
Techniques, systems, and devices are disclosed for implementing a portable lab system for PCR testing. An example method for operating an integrated thermal cycling system includes depositing samples into the integrated thermal cycling system that includes a thermal cycling device and an electronic interface. The thermal cycling device includes multiple wells to receive the samples to be thermally cycled, a thermoelectric cooling (TEC) element connected to the multiple wells, a substrate on which the TEC element is positioned, and a controller coupled to the TEC element. The multiple wells are positioned within the substrate that includes a thermally conductive ground positioned between adjacent wells. Supplying power to the integrated thermal cycling system, via the electronic interface, allows the multiple wells to exchange heat with the substrate and for each well to operate independently from other wells.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 2293812
- Patent Number(s):
- 11806719
- Application Number:
- 17/816,886
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- DOE Contract Number:
- AC52-07NA27344
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 08/02/2022
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Beer, N. Reginald, and Johnson, Gary. Integrated solid-state rapid thermo-cycling system. United States: N. p., 2023.
Web.
Beer, N. Reginald, & Johnson, Gary. Integrated solid-state rapid thermo-cycling system. United States.
Beer, N. Reginald, and Johnson, Gary. Tue .
"Integrated solid-state rapid thermo-cycling system". United States. https://www.osti.gov/servlets/purl/2293812.
@article{osti_2293812,
title = {Integrated solid-state rapid thermo-cycling system},
author = {Beer, N. Reginald and Johnson, Gary},
abstractNote = {Techniques, systems, and devices are disclosed for implementing a portable lab system for PCR testing. An example method for operating an integrated thermal cycling system includes depositing samples into the integrated thermal cycling system that includes a thermal cycling device and an electronic interface. The thermal cycling device includes multiple wells to receive the samples to be thermally cycled, a thermoelectric cooling (TEC) element connected to the multiple wells, a substrate on which the TEC element is positioned, and a controller coupled to the TEC element. The multiple wells are positioned within the substrate that includes a thermally conductive ground positioned between adjacent wells. Supplying power to the integrated thermal cycling system, via the electronic interface, allows the multiple wells to exchange heat with the substrate and for each well to operate independently from other wells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {11}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Rapid Microfluidic Thermal Cycler for Nucleic Acid Amplification
patent-application, September 2009
- Beer, Neil Reginald; Vafai, Kambiz
- US Patent Application 12/270348; 20090226972
Method for the complete chemical synthesis and assembly of genes and genomes
patent-application, September 2003
- Evans, Glen A.
- US Patent Application 10/322360; 20030165946
Portable Rapid Microfluidic Thermal Cycler for Extremely Fast Nucleic Acid Amplification
patent-application, September 2009
- Beer, Neil Reginald; Vafai, Kambiz; Mahjoob, Shadi
- US Patent Application 12/270030; 20090226971
Thermal device, system, and method, for fluid processing device
patent, September 2009
- Shigeura, John; Desmond, Sean Matthew; Cox, David M.
- US Patent Document 7,585,663
Isothermal surface production and regulation for high heat flux applications utilizing porous inserts
journal, August 2001
- Khanafer, K.; Vafai, K.
- International Journal of Heat and Mass Transfer, Vol. 44, Issue 15, p. 2933-2947
A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro
journal, February 2004
- Wang, Yan; Prosen, Dennis E.; Mei, Li
- Nucleic Acids Research, Vol. 32, Issue 3, p. 1197-1207
On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008
- Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna
- Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858
Use of temperature control devices in miniaturized planar column devices and miniaturized total analysis systems
patent, June 1997
- Kaltenbach, Patrick; Swedberg, Sally A.; Witt, Klaus
- US Patent Document 5,641,400
Thermal cycler for microfluidic array assays
patent-application, May 2006
- Yoder, Karl; Brenan, Colin; Linton, John
- US Patent Application 11/227425; 20060094108
Real-time Stadium and Sports Weather Network
patent-application, October 2008
- Copley, Rhonda L.
- US Patent Application 11/740460; 20080270030
Sleeve reaction chamber system
patent, August 2009
- Northrup, M. Allen; Beeman, Barton V.; Benett, William J.
- US Patent Document 7,578,976
Development of a High-Speed Real-Time Polymerase Chain Reaction System Using a Circulating Water-Based Rapid Heat-Exchange
journal, June 2010
- Terazono, Hideyuki; Takei, Hiroyuki; Hattori, Akihiro
- Japanese Journal of Applied Physics, Vol. 49, Issue 6, Article No. 06GM05
Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
journal, June 2007
- Zhang, Chunsun; Xing, Da
- Nucleic Acids Research, Vol. 35, Issue 13, p. 4223-4237
Dual-sided thermal cycler
patent-application, September 2010
- Shigeura, John S.; Desmond, Sean M.; Cox, David M.
- US Patent Application 12/786063; 20100233763
Pumped fluid cooling system and method
patent-application, March 2006
- Werner, Douglas; Goodson, Kenneth; Munch, Mark
- US Patent Application 10/927800; 20060042785
Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes
journal, June 2006
- Neuzil, Pavel; Zhang, Chunyan; Pipper, Juergen
- Nucleic Acids Research, Vol. 34, Issue 11, p. e77-e77
Crank Arm Stop for a Cam Reel
patent-application, January 2008
- Jones, Dennis J.
- US Patent Application 11/772585; 20080022647
Performance evaluation of thermal cyclers for PCR in a rapid cycling condition
journal, April 2008
- Ho Kim, Young; Yang, Inchul; Bae, Young-Seuk
- BioTechniques, Vol. 44, Issue 4, p. 495-505
High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets
journal, December 2008
- Kiss, Margaret Macris; Ortoleva-Donnelly, Lori; Beer, N. Reginald
- Analytical Chemistry, Vol. 80, Issue 23, p. 8975-8981
Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
patent-application, July 2008
- Beer, Neil; Hindson, Benjamin; Colson, Billy
- US Patent Application 11/650363; 20080166793
Thermal management in microfluidics using micro-Peltier junctions
journal, October 2005
- Maltezos, George; Johnston, Matthew; Scherer, Axel
- Applied Physics Letters, Vol. 87, Issue 15, Article No. 154105
On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets
journal, November 2007
- Beer, N. Reginald; Hindson, Benjamin J.; Wheeler, Elizabeth K.
- Analytical Chemistry, Vol. 79, Issue 22, p. 8471-8475
End-complementary polymerase reaction
patent, November 1998
- Stemmer, Willem P.; Lipshutz, Robert J.
- US Patent Document 5,834,252
Streaming-Based Micro/Mini Channel Electronic Cooling Techniques
patent-application, April 2010
- Zhang, Zongqin
- US Patent Application 12/345699; 20100091459
Method of manufacturing microfluidic devices
patent, March 1999
- McReynolds, Richard J.
- US Patent Document 5,882,465
Kinetics of the DNA polymerase pyrococcus kodakaraensis
journal, June 2006
- Griep, M. A.; Kotera, C. A.; Nelson, R. M.
- Chemical Engineering Science, Vol. 61, Issue 12, p. 3885-3892
Microdroplet-based PCR enrichment for large-scale targeted sequencing
journal, November 2009
- Tewhey, Ryan; Warner, Jason B.; Nakano, Masakazu
- Nature Biotechnology, Vol. 27, Issue 11, p. 1025-1031
Solid state rapid thermocycling
patent, May 2014
- Beer, Neil Reginald; Spadaccini, Christopher M.
- US Patent Document 8,720,209
Nano/Microfluidics for diagnosis of infectious diseases in developing countries
journal, March 2010
- Lee, Won Gu; Kim, Yun-Gon; Chung, Bong Geun
- Advanced Drug Delivery Reviews, Vol. 62, Issue 4-5, p. 449-457
Molecular diagnostic assay system
patent-application, January 2017
- Dority, Doug; Phan, Tien; Fromm, David
- US Patent Application 15/217920; 20170021356
Apparatus for heating and cooling at food serving stations
patent, February 2010
- Steffensen, Chris; Lyon, Tyler; Winegar, Daniel
- US Patent Document 7,665,311
Method for recombining polynucleotides
patent-application, August 2002
- Slater, Steven C.; Ng, Wing-On; Feng, Guo Hong
- US Patent Application 09/746432; 20020119535
Method for carrying out a biochemical protocol in continuous flow in a microreactor
patent-application, December 2005
- Fouillet, Yves; Vauchier, Claude; Clerc, Jean-Frederic
- US Patent Application 11/197040; 20050282224
Novel High-Speed Real-Time PCR Method (Hyper-PCR): Results from Its Application to Adenovirus Diagnosis
journal, January 2010
- Fujimoto, Tsuguto; Konagaya, Masami; Enomoto, Miki
- Japanese Journal of Infectious Diseases, Vol. 63, Issue 1
Temperature variation apparatus
patent-application, April 2010
- Wu, Yu-Min; Tsai, Yi-Chin; Liao, Kwan-Chiao
- US Patent Application 12/453306; 20100101237
Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification
journal, May 2008
- Mahjoob, Shadi; Vafai, Kambiz; Beer, N. Reginald
- International Journal of Heat and Mass Transfer, Vol. 51, Issue 9-10, p. 2109-2122