Modulating laccase enzyme to regulate cell wall biosynthesis and recalcitrance in plants
Abstract
This disclosure provides genetically modified plants having desirable levels of sugar release and syringyl/guaiacyl (S/G) ratio; methods of genetically modifying plants to modulate sugar release and S/G ratio; and uses of such plants. The inventors have determined that genetic modification of a laccase gene (LAC2) from Populus, encoded by locus Potri.008G064000 resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. Plants with altered sugar release, and S/G ratio, based on modulation of the expression or activity of the LAC2 gene, have divergent uses including pulp and paper production, and biofuel and bioproducts production.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568145
- Patent Number(s):
- 10246719
- Application Number:
- 15/647,819
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 07/12/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Chen, Jin-Gui, Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Tuskan, Gerald A., and Bryan, Anthony Christian. Modulating laccase enzyme to regulate cell wall biosynthesis and recalcitrance in plants. United States: N. p., 2019.
Web.
Chen, Jin-Gui, Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Tuskan, Gerald A., & Bryan, Anthony Christian. Modulating laccase enzyme to regulate cell wall biosynthesis and recalcitrance in plants. United States.
Chen, Jin-Gui, Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Tuskan, Gerald A., and Bryan, Anthony Christian. Tue .
"Modulating laccase enzyme to regulate cell wall biosynthesis and recalcitrance in plants". United States. https://www.osti.gov/servlets/purl/1568145.
@article{osti_1568145,
title = {Modulating laccase enzyme to regulate cell wall biosynthesis and recalcitrance in plants},
author = {Chen, Jin-Gui and Gunter, Lee E. and Jawdy, Sara S. and Yang, Xiaohan and Tuskan, Gerald A. and Bryan, Anthony Christian},
abstractNote = {This disclosure provides genetically modified plants having desirable levels of sugar release and syringyl/guaiacyl (S/G) ratio; methods of genetically modifying plants to modulate sugar release and S/G ratio; and uses of such plants. The inventors have determined that genetic modification of a laccase gene (LAC2) from Populus, encoded by locus Potri.008G064000 resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. Plants with altered sugar release, and S/G ratio, based on modulation of the expression or activity of the LAC2 gene, have divergent uses including pulp and paper production, and biofuel and bioproducts production.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 02 00:00:00 EDT 2019},
month = {Tue Apr 02 00:00:00 EDT 2019}
}
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