Title: Production of oil in vegetative tissues

Abstract

The present invention relates to compositions and methods for providing RNA interference (RNAi) vectors comprising trigalactosyldiacylglycerol (tgd) biosynthesis enzyme constructs for increasing oil content of plants. Further, the use of tgd RNAi silencing vectors in combination with co-expression of heterologous oil regulating transcription factors, such as WRINKLED1, are contemplated to overcome the reduced growth and variable levels of embryonic lethality in plants with reduced TGD protein. Additionally, plants having reduced APS1, a gene encoding a major catalytic isoform of the small subunit of AGPase (AGP(-) plants), co-expressing a heterologous oil modulating transcription factor are contemplated for use in combination with plants having reduced TGD. Oil harvested from vegetative tissues of plants comprising vectors and genes of the present invention is contemplated for use in biofuel and biodiesel products.

Inventors:

Benning, Christoph; Sanjaya, Sedbrook J.; Changcheng, Xu

Issue Date:

Tue Jun 26 00:00:00 EDT 2018

Research Org.:

Michigan State Univ., East Lansing, MI (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1460544

Patent Number(s):

10006039

Application Number:

14/046,504

Assignee:

Board of Trustees of Michigan State University (East Lansing, MI)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/415 - from plants

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/8218 - {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
C12N15/8247 - {involving modified lipid metabolism, e.g. seed oil composition}
C12N9/1241 - {Nucleotidyltransferases
C12N9/14 - Hydrolases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y207/07027 - Glucose-1-phosphate adenylyltransferase
C12Y306/03 - acting on acid anhydrides

Show less

DOE Contract Number:  

FG02-07ER20356

Resource Type:

Patent

Resource Relation:

Patent File Date: 2013 Oct 04

Country of Publication:

United States

Language:

English