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Title: Modified host cells with efflux pumps

The present invention provides for a modified host cell comprising a heterologous expression of an efflux pump capable of transporting an organic molecule out of the host cell wherein the organic molecule at a sufficiently high concentration reduces the growth rate of or is lethal to the host cell.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1312679
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
Patent Number(s):
9,428,726
Application Number:
13/115,925
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2011 May 25
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Works referenced in this record:

Protein tolerance to random amino acid change
journal, June 2004
  • Guo, H. H.; Choe, J.; Loeb, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
  • DOI: 10.1073/pnas.0403255101

Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988
  • Lazar, E.; Watanabe, S.; Dalton, S.
  • Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252
  • DOI: 10.1128/MCB.8.3.1247

Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998
  • Hill, Margaret A.; Preiss, Jack
  • Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577
  • DOI: 10.1006/bbrc.1998.8301

Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53
journal, February 1999
  • Wacey, A. I.; Cooper, D. N.; Liney, D.
  • Human Genetics, Vol. 104, Issue 1, p. 15-22
  • DOI: 10.1007/s004390050904

Advanced biofuel production in microbes
journal, January 2010
  • Peralta-Yahya, Pamela P.; Keasling, Jay D.
  • Biotechnology Journal, Vol. 5, Issue 2, p. 147-162
  • DOI: 10.1002/biot.200900220

Biofuel alternatives to ethanol: pumping the microbial well
journal, July 2008
  • Fortman, J. L.; Chhabra, Swapnil; Mukhopadhyay, Aindrila
  • Trends in Biotechnology, Vol. 26, Issue 7, p. 375-381
  • DOI: 10.1016/j.tibtech.2008.03.008

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels
journal, December 2008
  • Lee, Sung Kuk; Chou, Howard; Ham, Timothy S.
  • Current Opinion in Biotechnology, Vol. 19, Issue 6, p. 556-563
  • DOI: 10.1016/j.copbio.2008.10.014

Challenges in Engineering Microbes for Biofuels Production
journal, February 2007

Assembly and Channel Opening in a Bacterial Drug Efflux Machine
journal, April 2008

Mechanisms of RND multidrug efflux pumps
journal, May 2009
  • Nikaido, Hiroshi; Takatsuka, Yumiko
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1794, Issue 5, p. 769-781
  • DOI: 10.1016/j.bbapap.2008.10.004

Directed evolution of a bacterial efflux pump: Adaptation of the E. coli TolC exit duct to the Pseudomonas MexAB translocase
journal, September 2006

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
journal, January 2008
  • Atsumi, Shota; Hanai, Taizo; Liao, James C.
  • Nature, Vol. 451, Issue 7174, p. 86-89
  • DOI: 10.1038/nature06450

Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol
journal, January 2008
  • Steen, Eric J.; Chan, Rossana; Prasad, Nilu
  • Microbial Cell Factories, Vol. 7, Issue 1, Article No. 36
  • DOI: 10.1186/1475-2859-7-36

MicrobesOnline: an integrated portal for comparative and functional genomics
journal, November 2009
  • Dehal, P. S.; Joachimiak, M. P.; Price, M. N.
  • Nucleic Acids Research, Vol. 38, Issue suppl_1, p. D396-D400
  • DOI: 10.1093/nar/gkp919

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997
  • Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
  • Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
  • DOI: 10.1093/nar/25.17.3389

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006
  • Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki
  • Molecular Systems Biology, Vol. 2, Article No. 2006.0008
  • DOI: 10.1038/msb4100050