Title: Methods and compositions for simultaneous saccharification and fermentation

Abstract

The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.

Inventors:

Ingram, Lonnie O'Neal; Zhou, Shengde

Issue Date:

Tue Apr 11 00:00:00 EDT 2006

Research Org.:

Univ. of Florida, Gainesville, FL (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1175699

Patent Number(s):

7026152

Application Number:

09/885,297

Assignee:

University of Florida Research Foundation, Inc. (Gainesville, FL)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P19/14 - produced by the action of a carbohydrase {
C12P7/10 - substrate containing cellulosic material

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/01004 - Cellulase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

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DOE Contract Number:  

FG02-96ER20222

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES