Title: Adjusting the pH of a pretreatment solution using carbon dioxide useful for integrating saccharification and fermentation

Abstract

The present invention provides for a method of fermenting or saccharifying a biomass comprising: (a) (i) contacting a biomass comprising a polysaccharide, and an ionic liquid (IL) to form a first solution, or (ii) providing the first solution comprising the biomass and the IL, (b) contacting the first solution and carbon dioxide such that the first solution results in a lower pH, (c) introducing (i) an enzyme capable of enzymatically to breakdown at least one bond in the polysaccharide or a breakdown product of the polysaccharide, and/or (ii) a microorganism that capable of producing the enzyme and/or fermenting the polysaccharide or a breakdown product of the polysaccharide, such that the polysaccharide is at least partially broken down and the first solution is transformed into a second solution.

Inventors:

Sun, Jian; Shi, Jian; Simmons, Blake A.; Singh, Seema

Issue Date:

2020-11-17

Research Org.:

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1771635

Patent Number(s):

10837038

Application Number:

15/539,614

Assignee:

National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08B - POLYSACCHARIDES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/065 - {with microorganisms other than yeasts}

C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08B - POLYSACCHARIDES

C08B1/003 - {Preparation of cellulose solutions, i.e. dopes, with different possible solvents, e.g. ionic liquids

C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08H - DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS

C08H8/00 - Macromolecular compounds derived from lignocellulosic materials {

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P2201/00 - Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
C12P7/10 - substrate containing cellulosic material
C12P19/02 - Monosaccharides
C12P7/649 - {Biodiesel, i.e. Fatty acid alkyl esters}
C12P19/14 - produced by the action of a carbohydrase {

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DOE Contract Number:  

AC02-05CH11231

Resource Type:

Patent

Resource Relation:

Patent File Date: 12/23/2015

Country of Publication:

United States

Language:

English