Cysteine-containing peptide tag for site-specific conjugation of proteins
Abstract
The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety bound to the targeting moiety; the biological conjugate having a covalent bond between the thiol group of SEQ ID NO:2 and a functional group in the binding moiety. The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety that comprises an adapter protein, the adapter protein having a thiol group; the biological conjugate having a disulfide bond between the thiol group of SEQ ID NO:2 and the thiol group of the adapter protein. The present invention is also directed to biological sequences employed in the above biological conjugates, as well as pharmaceutical preparations and methods using the above biological conjugates.
- Inventors:
-
- W. Simsbury, CT
- Issue Date:
- Research Org.:
- SibTech, Inc. (Newington, CT)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1014528
- Patent Number(s):
- 7355019
- Application Number:
- 11/083,508
- Assignee:
- SibTech, Inc. (Newington, CT)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- DOE Contract Number:
- FG02-02ER83520
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Backer, Marina V, and Backer, Joseph M. Cysteine-containing peptide tag for site-specific conjugation of proteins. United States: N. p., 2008.
Web.
Backer, Marina V, & Backer, Joseph M. Cysteine-containing peptide tag for site-specific conjugation of proteins. United States.
Backer, Marina V, and Backer, Joseph M. Tue .
"Cysteine-containing peptide tag for site-specific conjugation of proteins". United States. https://www.osti.gov/servlets/purl/1014528.
@article{osti_1014528,
title = {Cysteine-containing peptide tag for site-specific conjugation of proteins},
author = {Backer, Marina V and Backer, Joseph M},
abstractNote = {The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety bound to the targeting moiety; the biological conjugate having a covalent bond between the thiol group of SEQ ID NO:2 and a functional group in the binding moiety. The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety that comprises an adapter protein, the adapter protein having a thiol group; the biological conjugate having a disulfide bond between the thiol group of SEQ ID NO:2 and the thiol group of the adapter protein. The present invention is also directed to biological sequences employed in the above biological conjugates, as well as pharmaceutical preparations and methods using the above biological conjugates.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 08 00:00:00 EDT 2008},
month = {Tue Apr 08 00:00:00 EDT 2008}
}
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