Title: Cysteine-containing peptide tag for site-specific conjugation of proteins

Abstract

The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety bound to the targeting moiety; the biological conjugate having a covalent bond between the thiol group of SEQ ID NO:2 and a functional group in the binding moiety. The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety that comprises an adapter protein, the adapter protein having a thiol group; the biological conjugate having a disulfide bond between the thiol group of SEQ ID NO:2 and the thiol group of the adapter protein. The present invention is also directed to biological sequences employed in the above biological conjugates, as well as pharmaceutical preparations and methods using the above biological conjugates.

Inventors:

Backer, Marina V ^[1]; Backer, Joseph M ^[1]

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+ Show Inventor Affiliations

1. Simsbury, CT

Issue Date:

2010-10-05

Research Org.:

SibTech, Inc. (Brookfield, CT)

Sponsoring Org.:

USDOE

OSTI Identifier:

1016149

Patent Number(s):

7807778

Application Number:

12/075,811

Assignee:

SibTech, Inc. (Brookfield, CT)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K47/60 - the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
A61K47/642 - {the peptide or protein in the drug conjugate being a cytokine, e.g. IL2, chemokine, growth factors or interferons being the inactive part of the conjugate}
A61K49/0032 - {Methine dyes, e.g. cyanine dyes}
A61K49/0056 - {Peptides, proteins, polyamino acids}
A61K51/088 - {conjugates with carriers being peptides, polyamino acids, proteins

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P9/00 - Drugs for disorders of the cardiovascular system

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/4721 - {Lipocortins}
C07K14/52 - Cytokines
C07K2319/00 - Fusion polypeptide
C07K2319/01 - containing a localisation/targetting motif

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C12N9/22 - Ribonucleases {RNAses, DNAses
C12N9/96 - Stabilising an enzyme by forming an adduct or a composition

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DOE Contract Number:  

FG02-02ER83520

Resource Type:

Patent

Country of Publication:

United States

Language:

English