Data from Development of a CRISPR/Cas9 System for High-Efficiency Multiplexed Gene Deletion in Rhodosporidium toruloides

Schultz, J. Carl; Cao, Mingfeng; Zhao, Huimin

doi:10.13012/B2IDB-7217468_V1

Title: Data from Development of a CRISPR/Cas9 System for High-Efficiency Multiplexed Gene Deletion in Rhodosporidium toruloides

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Abstract

The oleaginous yeast Rhodosporidium toruloides is considered a promising candidate for production of chemicals and biofuels thanks to its ability to grow on lignocellulosic biomass, and its high production of lipids and carotenoids. However, efforts to engineer this organism are hindered by a lack of suitable genetic tools. Here we report the development of a CRISPR/Cas9 system for genome editing in R. toruloides based on a fusion 5S rRNA–tRNA promoter for guide RNA (gRNA) expression, capable of greater than 95% gene knockout for various genetic targets. Additionally, multiplexed double‐gene knockout mutants were obtained using this method with an efficiency of 78%. This tool can be used to accelerate future metabolic engineering work in this yeast.

Authors:

Schultz, J. Carl;

;

Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL
Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Publication Date:: Tue Apr 30 00:00:00 UTC 2019

DOE Contract Number:: SC0018420

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois Urbana-Champaign

Sponsoring Org.:: U.S. Department of Energy (DOE)

Subject:: Conversion; Genome Engineering; Genomics; Transcriptomics

OSTI Identifier:: 3013653

DOI:: https://doi.org/10.13012/B2IDB-7217468_V1

Citation Formats


                    Schultz, J. Carl, Cao, Mingfeng, and Zhao, Huimin. Data from Development of a CRISPR/Cas9 System for High-Efficiency Multiplexed Gene Deletion in Rhodosporidium toruloides.  United States: N. p., 2019. 
        Web.  doi:10.13012/B2IDB-7217468_V1.

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                    Schultz, J. Carl, Cao, Mingfeng, & Zhao, Huimin. Data from Development of a CRISPR/Cas9 System for High-Efficiency Multiplexed Gene Deletion in Rhodosporidium toruloides.  United States.  doi:https://doi.org/10.13012/B2IDB-7217468_V1

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                    Schultz, J. Carl, Cao, Mingfeng, and Zhao, Huimin. 2019.  
"Data from Development of a CRISPR/Cas9 System for High-Efficiency Multiplexed Gene Deletion in Rhodosporidium toruloides".  United States.  doi:https://doi.org/10.13012/B2IDB-7217468_V1.  https://www.osti.gov/servlets/purl/3013653. Pub date:Tue Apr 30 00:00:00 UTC 2019

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@article{osti_3013653,

  title        = {Data from Development of a CRISPR/Cas9 System for High-Efficiency Multiplexed Gene Deletion in Rhodosporidium toruloides},

  author       = {Schultz, J. Carl and Cao, Mingfeng and Zhao, Huimin},

  abstractNote = {The oleaginous yeast Rhodosporidium toruloides is considered a promising candidate for production of chemicals and biofuels thanks to its ability to grow on lignocellulosic biomass, and its high production of lipids and carotenoids. However, efforts to engineer this organism are hindered by a lack of suitable genetic tools. Here we report the development of a CRISPR/Cas9 system for genome editing in R. toruloides based on a fusion 5S rRNA–tRNA promoter for guide RNA (gRNA) expression, capable of greater than 95% gene knockout for various genetic targets. Additionally, multiplexed double‐gene knockout mutants were obtained using this method with an efficiency of 78%. This tool can be used to accelerate future metabolic engineering work in this yeast.},

  doi          = {10.13012/B2IDB-7217468_V1},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Apr 30 00:00:00 UTC 2019},

  month        = {Tue Apr 30 00:00:00 UTC 2019}

}

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DOI: https://doi.org/10.13012/B2IDB-7217468_V1

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