U.S. Department of EnergyOffice of Scientific and Technical Information
Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida -putative knockouts table
Abstract
Table S1, putative gene knockout targets in P. putida KT2440 to enhance vesiculation; Table S2, protein sequence identity of OmpA from E. coli K12 to P. putida KT2440 genes; Table S3, strains utilized in this study and corresponding construction details; Table S4, oligonucleotides utilized in this study; Table S5, plasmids utilized in this study; Table S6, sequences for mNeonGreen, tags, and codon-optimized genes; Figure S1, particle count per gCDW for KT2440 and knockout strains corresponding to data presented in Figure 1B; Figure S2, OD600 measurements of extracted MVs from KT2440 and knockout strains; Figure S3, particle count per gCDW for WT, ΔPP_4669, and ΔPP_1502; Figure S4, particle count per gCDW for KT2440 and knockout strains corresponding to data presented in Figure 3C; Figure S5, sizes of MVs corresponding to particle counts in Figure S4; Figure S6, particle count per gCDW for KT2440 grown on 20 mM glucose alone or 20 mM glucose plus 12.5 mM p-coumarate and 12.5 mM ferulate; Figure S7 and Figure S8, principal component analysis of the cellular fractions; Figure S9, heatmap of outer membrane proteins with differential abundance; and Figure S10, mNeonGreen (mNG) fluorescence signal for the cellular fraction and the extracellular fraction
- Authors:
- Publication Date:
- DOE Contract Number:
- AC05-00OR22725
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Subject:
- hypervesiculation; membrane; outer membrane vesicles; protein; proteomics; secretion; vesicles
- OSTI Identifier:
- 3013014
- DOI:
- https://doi.org/10.25983/CBI/3013014
Citation Formats
Wilkes, Rebecca A., Miller, Tarryn E., Waldbauer, Jacob, Zhou, Nanqing, Zhang, Lichun, DiBiase, Beth N., Kamat, Neha P., Aristilde, Ludmilla, Beckham, Gregg T., and Werner, Allison Z. Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida -putative knockouts table. United States: N. p., 2026.
Web. doi:10.25983/CBI/3013014.
Wilkes, Rebecca A., Miller, Tarryn E., Waldbauer, Jacob, Zhou, Nanqing, Zhang, Lichun, DiBiase, Beth N., Kamat, Neha P., Aristilde, Ludmilla, Beckham, Gregg T., & Werner, Allison Z. Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida -putative knockouts table. United States. doi:https://doi.org/10.25983/CBI/3013014
Wilkes, Rebecca A., Miller, Tarryn E., Waldbauer, Jacob, Zhou, Nanqing, Zhang, Lichun, DiBiase, Beth N., Kamat, Neha P., Aristilde, Ludmilla, Beckham, Gregg T., and Werner, Allison Z. 2026.
"Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida -putative knockouts table". United States. doi:https://doi.org/10.25983/CBI/3013014. https://www.osti.gov/servlets/purl/3013014. Pub date:Wed Jan 14 00:00:00 UTC 2026
@article{osti_3013014,
title = {Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida -putative knockouts table},
author = {Wilkes, Rebecca A. and Miller, Tarryn E. and Waldbauer, Jacob and Zhou, Nanqing and Zhang, Lichun and DiBiase, Beth N. and Kamat, Neha P. and Aristilde, Ludmilla and Beckham, Gregg T. and Werner, Allison Z.},
abstractNote = {Table S1, putative gene knockout targets in P. putida KT2440 to enhance vesiculation; Table S2, protein sequence identity of OmpA from E. coli K12 to P. putida KT2440 genes; Table S3, strains utilized in this study and corresponding construction details; Table S4, oligonucleotides utilized in this study; Table S5, plasmids utilized in this study; Table S6, sequences for mNeonGreen, tags, and codon-optimized genes; Figure S1, particle count per gCDW for KT2440 and knockout strains corresponding to data presented in Figure 1B; Figure S2, OD600 measurements of extracted MVs from KT2440 and knockout strains; Figure S3, particle count per gCDW for WT, ΔPP_4669, and ΔPP_1502; Figure S4, particle count per gCDW for KT2440 and knockout strains corresponding to data presented in Figure 3C; Figure S5, sizes of MVs corresponding to particle counts in Figure S4; Figure S6, particle count per gCDW for KT2440 grown on 20 mM glucose alone or 20 mM glucose plus 12.5 mM p-coumarate and 12.5 mM ferulate; Figure S7 and Figure S8, principal component analysis of the cellular fractions; Figure S9, heatmap of outer membrane proteins with differential abundance; and Figure S10, mNeonGreen (mNG) fluorescence signal for the cellular fraction and the extracellular fraction},
doi = {10.25983/CBI/3013014},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 14 00:00:00 UTC 2026},
month = {Wed Jan 14 00:00:00 UTC 2026}
}