RhizoGrid Indexed Sorghum Rhizosphere Multi-Omics

Anderson, Lindsey N.; Anthony, Winston E.; Purohit, Sumit; Battu, Anil; Fonseca-García, Citlali; Tate, Kylee; Farris, Yuliya; Varga, Tamas; Coleman-Derr, Devin; Egbert, Robert G; Handakumbura, Pubudu

doi:10.25584/PerConSFA/3008954

Title: RhizoGrid Indexed Sorghum Rhizosphere Multi-Omics

Dataset
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Abstract

PerCon SFA project data dentification of spatially resolved biomarkers of drought in Sorghum bicolor rhizosphere molecular-microbe interactions using a novel root cartography "RhizoGrid" system for sampling plants under drought and control conditions across 10 equally sized root zone environments (4 quadrants each). Each quadrant was sampled and processed for 16S amplicon, metabolomics, and X-ray computed tomography (XCT). Data download includes experimental metadata and results files for 16S rRNA sequence analysis of microbial community assembly (processed data files), liquid chromatography mass spectrometry (LC-MS) metabolomics analysis of microbial community root exudates (processed data files), X-ray computed tomography (XCT) spatial gradient analysis (raw and processed data files) of microbial community composition, and related computational modeling outputs.

Authors:

;

; Farris, Yuliya;

; Coleman-Derr, Devin;

;

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
University of California, Berkeley, CA (United States)

Publication Date:: Tue Dec 16 23:00:00 EST 2025

Other Number(s):: PNNL-SA-216279
MSV000100162; SRP654059

DOE Contract Number:: AC05-76RL01830

Research Org.:: PNNL (PNNL2)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Causal Mediation; Drought Stress; Graph Attention Models; Microbe-Metabolite Interaction; Microbiome Assembly; RhizoGrid; Rhizosphere

OSTI Identifier:: 3008954

DOI:: https://doi.org/10.25584/PerConSFA/3008954

Citation Formats


                    Anderson, Lindsey N., Anthony, Winston E., Purohit, Sumit, Battu, Anil, Fonseca-García, Citlali, Tate, Kylee, Farris, Yuliya, Varga, Tamas, Coleman-Derr, Devin, Egbert, Robert G, and Handakumbura, Pubudu. RhizoGrid Indexed Sorghum Rhizosphere Multi-Omics.  United States: N. p., 2025. 
        Web.  doi:10.25584/PerConSFA/3008954.

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                    Anderson, Lindsey N., Anthony, Winston E., Purohit, Sumit, Battu, Anil, Fonseca-García, Citlali, Tate, Kylee, Farris, Yuliya, Varga, Tamas, Coleman-Derr, Devin, Egbert, Robert G, & Handakumbura, Pubudu. RhizoGrid Indexed Sorghum Rhizosphere Multi-Omics.  United States.  doi:https://doi.org/10.25584/PerConSFA/3008954

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                    Anderson, Lindsey N., Anthony, Winston E., Purohit, Sumit, Battu, Anil, Fonseca-García, Citlali, Tate, Kylee, Farris, Yuliya, Varga, Tamas, Coleman-Derr, Devin, Egbert, Robert G, and Handakumbura, Pubudu. 2025.  
"RhizoGrid Indexed Sorghum Rhizosphere Multi-Omics".  United States.  doi:https://doi.org/10.25584/PerConSFA/3008954.  https://www.osti.gov/servlets/purl/3008954. Pub date:Tue Dec 16 23:00:00 EST 2025

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@article{osti_3008954,

  title        = {RhizoGrid Indexed Sorghum Rhizosphere Multi-Omics},

  author       = {Anderson, Lindsey N. and Anthony, Winston E. and Purohit, Sumit and Battu, Anil and Fonseca-García, Citlali and Tate, Kylee and Farris, Yuliya and Varga, Tamas and Coleman-Derr, Devin and Egbert, Robert G and Handakumbura, Pubudu},

  abstractNote = {PerCon SFA project data dentification of spatially resolved biomarkers of drought in Sorghum bicolor rhizosphere molecular-microbe interactions using a novel root cartography "RhizoGrid" system for sampling plants under drought and control conditions across 10 equally sized root zone environments (4 quadrants each). Each quadrant was sampled and processed for 16S amplicon, metabolomics, and X-ray computed tomography (XCT). Data download includes experimental metadata and results files for 16S rRNA sequence analysis of microbial community assembly (processed data files), liquid chromatography mass spectrometry (LC-MS) metabolomics analysis of microbial community root exudates (processed data files), X-ray computed tomography (XCT) spatial gradient analysis (raw and processed data files) of microbial community composition, and related computational modeling outputs.},

  doi          = {10.25584/PerConSFA/3008954},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Dec 16 23:00:00 EST 2025},

  month        = {Tue Dec 16 23:00:00 EST 2025}

}

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DOI: https://doi.org/10.25584/PerConSFA/3008954

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